FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-gonad-RNA-7_S49_L007_R1_001.fastp-trim.20220908.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-gonad-RNA-7_S49_L007_R1_001.fastp-trim.20220908.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1327
Sequences flagged as poor quality0
Sequence length35-151
%GC39

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC30022.607385079125848TruSeq Adapter, Index 6 (100% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC997.46043707611153TruSeq Adapter, Index 6 (100% over 50bp)
GCTCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC80.6028636021100227TruSeq Adapter, Index 6 (98% over 50bp)
ACAGTGTTCGCCCATCTTCCAACTGCTTGCCAGCGAAGATCAATCTCTGC80.6028636021100227No Hit
GCGGTTTCGAACTTCCATAAAGCGATATCAATGGTGATACCACGTTCTCT40.30143180105501133No Hit
AAATAGCATTTCTAAATATCCTTGATATACACGATCTATAGTAACTGTGT40.30143180105501133No Hit
GTCATCCCAAGAAAAGCTAAGCCGGTACTTTGACGAACGCCGTTTTTCAC40.30143180105501133No Hit
GGTTTGCAATGAGATTTCTTTATTGTTTAAGACAACACAATCATTTATTC40.30143180105501133No Hit
TTTACACTTCCTTCTCTTCGAACTACTTAATTATATTTCCTCGCCCAAAA30.22607385079125847No Hit
CGAAACCAGTCATACCGGACTGACTTGCGTAAATATTTGTTTTCGCTTGT30.22607385079125847No Hit
CTCTGTCGTGTATTAATGTATTTAATACACATGTTCGGTTGCGCCACTCA30.22607385079125847No Hit
TCAGAAACACTTCTTTCATGCTGATTGATATTGCGTTTCATTTGATTTTC30.22607385079125847No Hit
GTTCTCGTTGCCTTCTCTCAGTGTGGAATGCCCACTGTCTATATCTTCTA30.22607385079125847No Hit
CTTTGGAATTTCCGTCACGACGCACTCGGCTGTCGTCAACAACGAGGCGA30.22607385079125847No Hit
CGAGGGCGGAGCCTTAGAACTGTAGGAAGGCGGAGCTATGTAATTGTATG30.22607385079125847No Hit
GTTGGGACCCATAATTCTGACCTTCTGTCCTGTACCTACAGTACCAGAAA30.22607385079125847No Hit
GTAACTGCTTACATCAACTGCTATTTAATGATACTTGGCAAAATCAAACA30.22607385079125847No Hit
ATTCTGTGCATAAATTTAGGCATGTGTAGCACAACTCTCTTTACGGATCT30.22607385079125847No Hit
CACACCAATCAAATCTTTAATAGTAAAATAATTATGAAAAGGGACTTTAT30.22607385079125847No Hit
GCAAATACTTGTCTTACAGTGTGTTACTAAGAGTTGATATGTTCTGTTAT30.22607385079125847No Hit
GTTAGTTTAAAAGCAAGAACCCAAATCCCGCTTTATACATGTCATTTAAG30.22607385079125847No Hit
GCAATATAAAAAACACCTATTTACAGTATATATAGCTTTATCCATTTACA20.15071590052750566No Hit
GCAGTTTCTCGCCGCGTTTCCTTTCAGCATGCAACTGCTTCTTCAGATCT20.15071590052750566No Hit
CCCATATCTACACATACATTACCACGTACAAGACCTTCAGTACCGTCCAT20.15071590052750566No Hit
TTTAGACCTAGCGAGGGTAAAGTCCAACAATGCATCACATCCTCCGACCC20.15071590052750566No Hit
GTCCATTCCATCTTTAGGAGTTTGTAGATAAGGATCCATCCACCATTGCC20.15071590052750566No Hit
ATCCCCATCATCACTCTCATCATCAGACTGATCGACAAGGTCACCATAGC20.15071590052750566No Hit
CAAATTCACAAAACAAACCTCATTGCAGGCAGTTCTTTTCATGCAATATT20.15071590052750566No Hit
AAGAAATACACATTTCGATCGAAGTTATTTATTTTCTCGCTTTTTTAAAA20.15071590052750566No Hit
GTGGTACCTCCGGAGAGGACAGTGTTGGCGTACAGATCCTTACGGATGTC20.15071590052750566No Hit
GGATTCGAGAGCGGAGCGGATCTCTTCGCATTCTCCCTGTACAACAACAA20.15071590052750566No Hit
GATTACCATATTTTATTGATAATGATGATTGTAAGCCACAAGAGCTGTGA20.15071590052750566No Hit
CTCAACTTCTCACCGAGAGATGAGAGAAGATGTCTCAATTCGGCGCCCGA20.15071590052750566No Hit
GTTATCTTAAGTTTATATCAATAACTATTATCAGTTAGCATGCAAACTTT20.15071590052750566No Hit
GTTTCAAATCATTGACAAACTCTGCGGACTGTTGATGCCGCATTTCTGAA20.15071590052750566No Hit
AGACAAAGTTCAACAAGCCACAAGTCTTAGAGCCTATACCATATGAGTTT20.15071590052750566No Hit
GATCGGAAGAGCACACGTCTGAACTCTAGTCACGCCAATATCTCGTATGC20.15071590052750566TruSeq Adapter, Index 6 (98% over 50bp)
CACTTAACAACAAATATTACAATTTCAACCTTTATTTAGCACTTAAAATA20.15071590052750566No Hit
CGGTAGCGTGTGTGACTTTTCATCCAGGTATCGTGAGGTCGGATCGACGC20.15071590052750566No Hit
TGTATTCAAACTATCGAAAACTGGAGAAATTAATTTATAATTTGAGTTAA20.15071590052750566No Hit
ATTCACAGTTGTAGAAATGAAATTAATAGCCCCCAAAATAGAAGATACCC20.15071590052750566No Hit
TTTCATTCAAGAGAGTTTTGTCTGTACAGTGTGTACACTGATGACAAAGT20.15071590052750566No Hit
ATTTTTGTAATCAGATCTGTTTCTGCTTGGAGAGACTTCAGTTGATTCAC20.15071590052750566No Hit
GCCAAATCCTTGTACAACGTTCACGAACCTCCTGTTATACTGCATGCGTC20.15071590052750566No Hit
CCGACAACAGACGACGAACTCCAGGCGATCACAAACGCTCACCCATGACC20.15071590052750566No Hit
TTAACTTCAAGATGTTTCTTAGCTGCCATGTATCCCATGGTTGATGAGTC20.15071590052750566No Hit
GTTAATCCAGAAAAAGGTTTTCAGAGCTTCCGCTACATGTTTGTCGTCTG20.15071590052750566No Hit
GTTTGCTTGTGCATTTTCAAAACCATGTGTGCGAAAGTGCATATTCAATA20.15071590052750566No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATAACTCGTATGCC20.15071590052750566TruSeq Adapter, Index 6 (98% over 50bp)
TTAAAATCATAAACACACCTACAAAGTATCAAAAAAAATACTGTGACACT20.15071590052750566No Hit
GTTGAAAGCGATGCTAAACACCATACAACTAATATACCAAATATGAACAA20.15071590052750566No Hit
ATAAAACCTGCGGTCTAAATACATGGGAGATGTCGAGC20.15071590052750566No Hit
GCAGATTCCATTCCCAGGAAGGATGGCTGGAAAAGTGACTCTGGGCAACG20.15071590052750566No Hit
TAAGGTACTCGGCGTTGGCTCTGTTGGCGTTGTCAAGGGCAGCCTCGAGC20.15071590052750566No Hit
ACTTCGCTTTATGCTGGACGGTAGGTTGAAACATAAAACACATTTATGTT20.15071590052750566No Hit
AAAATCAATATTTTCATTCAAATATATATTGTACCTATTAGTCACAAAAA20.15071590052750566No Hit
TGTAAATTAGTGTCTATCAATTACTGAGCATATTTTTAACACTTTGTGAG20.15071590052750566No Hit
TGTTCATCGTTAAATTTGATTGATATTAAGTACTCTCCTGGCTCTGCCAC20.15071590052750566No Hit
CAGAGCCATACACCCCATCCGAAATAGAAAAAGATGCGCCTATATACTCT20.15071590052750566No Hit
TATTAAGGTAATAGAATAACCTAACTACTGACCTAAAAATTAAAACTCTC20.15071590052750566No Hit
TGAGTGCAACTGTTTTTGGTCTAAATCCAGAGTACCAGGTTCCAAATGTT20.15071590052750566No Hit
TGATGACCTGACCGTCGGGAAGTTCGTAACTCTTTTCCAGAGAGCTGGAT20.15071590052750566No Hit
GTCCAGTTCAGGTGTTCCTTGCTGCTTCACAACTAAATCATTCCCACACA20.15071590052750566No Hit
TATATGTATAATTCTTCTTTTGATGATGAGAATGTTGTATCACAAAGTCC20.15071590052750566No Hit
TTAGCTGCCAAACTTCCAGGTGTAACCCTGGCAATGGTGAGGGGGCTGCT20.15071590052750566No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.15071590052750566No Hit
GGCCTGCATAGGAGTGAATATTTACTACCATTTAAGAGATAATTTATGGT20.15071590052750566No Hit
GTTCACAACAACACTTAAATTCAGTTTGTGTTTATTTATTTATGTTTTTT20.15071590052750566No Hit
TACATCTCCATTAAATACAAAATTTTTAACGAAGGACCAGCCAAAAATAA20.15071590052750566No Hit
CGCCAACACAGGCATAGCAAGCACCAGCAAAAAAGCAGTGATAGCAACTG20.15071590052750566No Hit
AATAGAAAAAGATGCGCCTATATACTCTTCCAACTGAAGAACTGAAAAAT20.15071590052750566No Hit
CCATTGCGTCAAGTCGGGAACCACACCAGCGACCTCTGGCGGGAGTAGCA20.15071590052750566No Hit
ATATCCTAGAAACTTACTTCATATACTCTTGGAAACCTCACTTTTTATCGGGGAAAACTGAGAACAA20.15071590052750566No Hit
AAGAGGCTTCATCCAGCTCTTGCACTTCCATGTTGTTAATACTGGTGTTA20.15071590052750566No Hit
TTGGTGAACTTGCCAAATAATTAATCCTACAAGAGTTGATTTTATCAACA20.15071590052750566No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTTACGCCAATATCTCGTATGCC20.15071590052750566TruSeq Adapter, Index 6 (98% over 50bp)
CTACATTAGATGTCCAAAGGCTTCTATATGAAGTAAAGGGTTAAGTCAGT20.15071590052750566No Hit
CGACGGATCAAAAAATGAGGTATTAAAATTACGATCTGTTAATAATATTG20.15071590052750566No Hit
TATAGGTATCACCAGAAAAAAAATTATAACAAAGGCATGCGCAGTCACAA20.15071590052750566No Hit
CATGAAATACCAGAACAAGAGGGGTGGCCGTGTTGTTCTCCAGCCCATCC20.15071590052750566No Hit
GGGCAAAACCCCTACTGGAGGTCAACACCCGCCCTCAGCCAAAGATCCTA20.15071590052750566No Hit
TCTGGGCCTGGGGTGTCTCTGAGCCAGGGCCCAGA20.15071590052750566No Hit
AAAGTTCGAAAGAGAAAGGCAACTTTACAAAAAGATCGGCAAAGTGTATC20.15071590052750566No Hit
GTCAAAATTAAAGTATTAACTTTTTTTTTTTTAAATCAGACTTTCATTAA20.15071590052750566No Hit
AGGATATGGAAATTTAAGTTAACTTTCCTTTTCTTTCAGTACTTTGAATA20.15071590052750566No Hit
CAGGCATCCCCACATTGGCAAGATTTGCCGCAGCTACAGGCACCCTTTTC20.15071590052750566No Hit
GTCGAACTCGTTTTCGAGATTCGTGTGTTTCTTCTGGAGTGCCGAAAGGT20.15071590052750566No Hit
GCCCATATCTCCAGACACATTACTTCCCTGTGAGGGACTTCTCACAACAT20.15071590052750566No Hit
CAGGTTAGCTGTTCCGTTCAGTATAGTAGACTGCAATACTTCATAGGGAT20.15071590052750566No Hit
ACAAAACATCTCCGATACGATTCATTAGAACAGTAATCATCCCCGCCCCC20.15071590052750566No Hit
CTACAGTCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAG20.15071590052750566Illumina DpnII expression Sequencing Primer (96% over 30bp)
GAAGAAGTGAAGCAAGAGGCAGAAGAAATAAAAACAGAACCTGCTGTTGA20.15071590052750566No Hit
CTGTAAGGTCATTGCTGAATGAGGGAGATTTTGCTGATGTTGTGATGGCC20.15071590052750566No Hit
CGAACAGTGATCTATTGTCAATAGCAAACTAAAAACAAACCAATGAGTAA20.15071590052750566No Hit
ATTCTATTCTTAGCCGGAACCACACGGGCTCCAAACTTTTAAAAAGGAAA20.15071590052750566No Hit
TTGGGGTTGAGCGGGGCTTCGGTAAGTAGAACGGGGTGTTCCTCTGGGGC20.15071590052750566No Hit
TTAAATATGTGTTTTTGGGTATATACATGCAGTGTCTTGTTAGAGTAATA20.15071590052750566No Hit
AGGGCCTTCTTGTTCTTTCCAAACAAAACAAAAAACAAATTCTTAAAGCA20.15071590052750566No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)