FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-gonad-RNA-6_S41_L006_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-gonad-RNA-6_S41_L006_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1364
Sequences flagged as poor quality0
Sequence length100-151
%GC67

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC161.1730205278592376No Hit
TTACGTGCAACATTCCGACAATATACACAACTAGTACCAATCCCTCTAGT130.9530791788856305No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG130.9530791788856305Illumina Single End PCR Primer 1 (100% over 50bp)
GTACTTCTGTGACAAATATTGCAATAGTCGAGCACCCTGTCCCTTTGACA70.5131964809384164No Hit
AGAACCCCCGTCAATACAAAATTCCAGACTGGTTCCTCAACAGACAGAAG60.43988269794721413No Hit
GGGTACCCTGTACGCTATTAGTCGTGTACACTCTAACACCTGCATTGAAT60.43988269794721413No Hit
GAATGCATATATCATTATTCAGTTTGTTTTTGTAAATTATATACATGCGA40.2932551319648094No Hit
ACATCTTAACCTCTTCAGCAAAACTGTTACCATAGTTTGATACCTATTCG40.2932551319648094No Hit
TGGCCTCAGATCAACAAACAAGACAAAGATGTCTGAATTAGATATGATAT30.21994134897360706No Hit
TTGCGCATGATCAAGAAAAGGAAGGATAAGAAAACCATAGGACAGGTGGA30.21994134897360706No Hit
AATCGGTATGCTCTACCATCAGCCCACTACAGTGACAGTGATTTCGCGCA30.21994134897360706No Hit
GCAAAATATACCGGGTCAGATGATGAAGATGTCGATATCAAAGCTCTTCT30.21994134897360706No Hit
GGGACAGAAGGACAGCTACGTCGGTGATGAGGCCCAGAGTAAGAGAGGTA30.21994134897360706No Hit
CCCCCCCCCCCACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC20.1466275659824047No Hit
GCCTTTTTTACCATCTAGGGCCATGTCCGACTCCGAAGACCAACCGGAGA20.1466275659824047No Hit
CCCCCCCCCCCCCCCCACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC20.1466275659824047No Hit
GTCAAATATCAGTATTTACAAACCCGAAGATGATTTTTCTAGTAAAATCT20.1466275659824047No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCAC20.1466275659824047No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)