FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-gonad-RNA-6_S41_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-gonad-RNA-6_S41_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1364
Sequences flagged as poor quality0
Sequence length100-151
%GC72

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC1047.624633431085044No Hit
GCGACTGGTACCAAGCTTAGTTGTGTATATTGTCGGCATGTTGCACGGGA120.8797653958944283No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC100.7331378299120235TruSeq Adapter, Index 6 (100% over 50bp)
TGAAAACTGGTAATCACAGGGGAATACTTAAATTACCTGTATAACTAGAG70.5131964809384164No Hit
TCACACGCAGACCCCAATAATGTCGGAGACCTCTGTGGGCTCGGATTTTC60.43988269794721413No Hit
CCCCCCGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC60.43988269794721413No Hit
CACCACGCATTTGCAATATGCATGCACAATGATTAAATACATAAACAAAA50.36656891495601174No Hit
ACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC50.36656891495601174No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCG50.36656891495601174No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCACCCC50.36656891495601174No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCACCC40.2932551319648094No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCACCCCCCCCCCCCCC40.2932551319648094No Hit
CCTGTTTTCCTTAATCCCGTAACACATGCACATTTACTTTTGAATTTGCG40.2932551319648094No Hit
CCCGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC30.21994134897360706No Hit
TCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC30.21994134897360706No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCACCCCCCCCCCCCCCCCCCC30.21994134897360706No Hit
CCGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC30.21994134897360706No Hit
TCTTCATCATCGTCATCGTCATCGTCATCGTCATCGGACAATCCACGTTT30.21994134897360706No Hit
GTTAGCCTTGGGGTTGAGCGGGGCTTCGGTAAGTAGAACGGGGTGTTCCT30.21994134897360706No Hit
GCTGCCACAGTTACCGGATTGGGTTGCGAATGAGGATTCAGTAAACACTG30.21994134897360706No Hit
TTTAGTTATAAGAATACAAAACGGGAGAATAAAAGGCCCAATGCAAGTTG30.21994134897360706No Hit
CATACATCATAATAAAATTACGAAATGAATTGTCGTACGTTCCAATTGCG20.1466275659824047No Hit
CTAGATTATGTCAGAGACGTAAATACAGAGCTCCGGTTATATGAGAAAAT20.1466275659824047No Hit
CCCGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCACCCCCCCCCCCCCC20.1466275659824047No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCTCCCCCCCCCCC20.1466275659824047No Hit
CACCACGCATTTGCAATATGCATGTACAATGATTAAATACATAAACAAAA20.1466275659824047No Hit
TATTTTTAGAAAAATGTGTACTTTAACTATGGCAATGTTTTTTTTTTTCT20.1466275659824047No Hit
GGGGGTTGTTCTTGATTATGGCAGTCTGATGTACAAAAACGTCTTCTTTG20.1466275659824047No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCGCCCCCCCCCCCCCC20.1466275659824047No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCACCCCCCCCCCCCCCCCC20.1466275659824047No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)