FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-gonad-RNA-6_S41_L006_R1_001.fastp-trim.20220908.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-gonad-RNA-6_S41_L006_R1_001.fastp-trim.20220908.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences191
Sequences flagged as poor quality0
Sequence length100-151
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC2010.471204188481675No Hit
GCGACTGGTACCAAGCTTAGTTGTGTATATTGTCGGCATGTTGCACGGGA126.282722513089005No Hit
TGAAAACTGGTAATCACAGGGGAATACTTAAATTACCTGTATAACTAGAG73.664921465968586No Hit
TCACACGCAGACCCCAATAATGTCGGAGACCTCTGTGGGCTCGGATTTTC63.1413612565445024No Hit
CACCACGCATTTGCAATATGCATGCACAATGATTAAATACATAAACAAAA52.6178010471204187No Hit
CCTGTTTTCCTTAATCCCGTAACACATGCACATTTACTTTTGAATTTGCG42.094240837696335No Hit
TTTAGTTATAAGAATACAAAACGGGAGAATAAAAGGCCCAATGCAAGTTG31.5706806282722512No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC31.5706806282722512TruSeq Adapter, Index 6 (100% over 50bp)
TCTTCATCATCGTCATCGTCATCGTCATCGTCATCGGACAATCCACGTTT31.5706806282722512No Hit
GTTAGCCTTGGGGTTGAGCGGGGCTTCGGTAAGTAGAACGGGGTGTTCCT31.5706806282722512No Hit
GCTGCCACAGTTACCGGATTGGGTTGCGAATGAGGATTCAGTAAACACTG31.5706806282722512No Hit
GGGGGTTGTTCTTGATTATGGCAGTCTGATGTACAAAAACGTCTTCTTTG21.0471204188481675No Hit
ACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC21.0471204188481675No Hit
TCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC21.0471204188481675No Hit
CTAGATTATGTCAGAGACGTAAATACAGAGCTCCGGTTATATGAGAAAAT21.0471204188481675No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCACCCCCCCCCCCCCCCCC21.0471204188481675No Hit
CACCACGCATTTGCAATATGCATGTACAATGATTAAATACATAAACAAAA21.0471204188481675No Hit
TATTTTTAGAAAAATGTGTACTTTAACTATGGCAATGTTTTTTTTTTTCT21.0471204188481675No Hit
CATACATCATAATAAAATTACGAAATGAATTGTCGTACGTTCCAATTGCG21.0471204188481675No Hit
CCCGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC21.0471204188481675No Hit
CCCGACACCCCCCTCCCCCCCCCCCCCCCACCCACACCCCCCCACCCCCC10.5235602094240838No Hit
TTTGAATAAAAAAAAAGCAAAATATTTTGAAAAAAGTCTTACTTATATGT10.5235602094240838No Hit
GGGGATCGCTCTTCGAGTCACCACATACGTTTCCTCTCTTGATATCTTTA10.5235602094240838No Hit
GAAAAGTGCATACAATACTGTATAGTGTTCATCCAATTTCTGCATGAGTA10.5235602094240838No Hit
GCCACCTCTCAGACGGAGTACAAGATGGAGTGTTGACTCTTTCTGGATGT10.5235602094240838No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCACCCACCCCCCACC10.5235602094240838No Hit
TGAAATAACGTTTTAGATCTCCAGAAAGTTTTAAAAGACAATCTGCTTAA10.5235602094240838No Hit
CAGAACCAAATTTGTCAAAATTTCATTCTACATGTGTTGTTGCCTCCCTT10.5235602094240838No Hit
AAAAAAAACACAATGATAAACAGCCATCAAAGTATACGATACACAAACTA10.5235602094240838No Hit
GAATAAAGCAAGACAAATTATTAAAAGCTAGAATAATAATAACTTATGCC10.5235602094240838No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCAAC10.5235602094240838No Hit
CCCGGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCACGCCCCCCACC10.5235602094240838No Hit
ACCCGCCCCCCCCCACACCCCCCGCCCCCCGCCCCCCCCACCCCCCCCCC10.5235602094240838No Hit
TTTTAAAGTCATTGAGGGCTAAGAGACTATCAGTTACATAACTACTCTTT10.5235602094240838No Hit
TGGGTTTTCGTTTCAAGTTTACCACTCAAATGTAAATATCCATTATGTAA10.5235602094240838No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATACCTCGTATGC10.5235602094240838TruSeq Adapter, Index 6 (98% over 50bp)
CCGCGCCCCCCCCCCCCCCCCCTCCCCGCCCGGCCCCCCTACACCCCCCC10.5235602094240838No Hit
AAGATACCCCTTACTACGTTAAGCATAACAAAGAAATTCCGAAAAATAAG10.5235602094240838No Hit
GAAGAAGCTTTCAGTTTAACCAAATGTCCTTCATATCTAACGAAATTTAG10.5235602094240838No Hit
CCCCCCGCCCCCCCCCCCCCCCCCCCGCCCCCCCCCCCCCCCCCCCCCCA10.5235602094240838No Hit
CCCCCCGCCCCACCCCCCCCCCCCCCCCCCGCCCCCCCCCCCCCCCCCCA10.5235602094240838No Hit
ACCCCGGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC10.5235602094240838No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCCTTGTAATCTCGTATGCCG10.5235602094240838Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
GAAATGAACACAAACTTTAAATAAACTGTTTGTTTTGCTGTTACATTGGT10.5235602094240838No Hit
TTCTTACTAAGCATAAAAGTAGATTCTAAATGCTTAAATATTTACAAATA10.5235602094240838No Hit
TTACACTTTCTTTTACTTGTGTTGCACAACTGAGGTCAGTCAACACATAC10.5235602094240838No Hit
TTTGAACACAGCAGTTTCACGATTTCCTGCATACCTGCTGCTCTGGACTC10.5235602094240838No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCACCCCACCCCCCCCACCCCACCC10.5235602094240838No Hit
AATTCCATCAGATGCTGTTTGACGAAGTAGCTATACTGTAGAATAAGCAA10.5235602094240838No Hit
GGGGGTGGTTCTTGATTATGGCAGTCTGATGTACAAAAACGTCTTCTTTG10.5235602094240838No Hit
CCCACTTCACCTGTGCAACAATATAAGTATCTTGTACAACATATGAAAAT10.5235602094240838No Hit
CACCGCCCCCCCCCCCCCCCACCCCCCCCCCCACCCCCCACCCCTCCCCC10.5235602094240838No Hit
ACCCCCGCCCCCCCCCCCCCCCCCCACCCCCCCCCCCTCCCACGCCCCCC10.5235602094240838No Hit
CTTACGTTCATATAGTTTACAGTAACTGGTCTCTATAAAATACAAAAATA10.5235602094240838No Hit
TTTACATACACAATCTACCAGGTCAACATATCCTGAAACACCTATACTAG10.5235602094240838No Hit
CCAACATTTGGGTCACAATTTGTGTCCTCACTTAAATATGTTAAAAAAAC10.5235602094240838No Hit
CCCCCCCCCCCACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC10.5235602094240838No Hit
ACCCCCGCACCCCCCCCCCCCCCCCCCCCCCCCCCCACCCCCCCCCCCAC10.5235602094240838No Hit
CTTGGAACAGTCTTGTCTGTTAATCCGGGAATTTCTTGTTCACCTTTCTT10.5235602094240838No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCACCCCCCCC10.5235602094240838No Hit
CCCGCCCCCCCCCCCCCCCCCACACCACCCCCCCCCCCCCCCCCCCCCCC10.5235602094240838No Hit
GTGCTCCATAGCCACCGCCATAACCGCCACCGTAATCTTGGTACCTTCCG10.5235602094240838No Hit
CTAGCTTATGTCAGAGACGTAAATACAGAGCTCCGGTTATATGAGAAAAT10.5235602094240838No Hit
CTATAAAATAACATTTTAGACAACAAATTTGCAACATGTGTTACTCCATT10.5235602094240838No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCGGCCCCCCCCCCCCTCCCCC10.5235602094240838No Hit
GCCCCCCCCCCCCCCCCCCCCCCCCCCCACCCCCCACCCCCCCCCCCCCC10.5235602094240838No Hit
TGGAGGATATCTCTTCCATTTTCTTATAAATTTCGCTCCGTTTTTCTGTT10.5235602094240838No Hit
ACCCCCGCCCACGCCCCCCACCCCCCCCCCCCCCCCCCCCCCCCCCCCCC10.5235602094240838No Hit
GTTTTATGAGTCAAATTAGTATACAGCCAGCATAATAAGGTACACATTTT10.5235602094240838No Hit
CCCGCCCCCCCCCCCCCCCCCCCCAACACCCCCCCCCCCCCCCCCCCCCC10.5235602094240838No Hit
GTGACATTTATATTTCAATAACTGTGTTATGTTTAGTACAAGAGTGGCGT10.5235602094240838No Hit
GTTTTTTTCTCTTTAATAGCCACTACATACAATACAACTCATCTACTTGA10.5235602094240838No Hit
TTTTGTACTTTTGTTACTATGGCATCATGTTTCAAGGACCAGTAGTAAAA10.5235602094240838No Hit
GGAAGTAAAAGTAATTTATTACTTCTCCTTTAAGTTACATGTGGAGAAAA10.5235602094240838No Hit
ACGCCCCCCCCCCCCCCCCCCCCCCCCCCCCGCCCCCCCCCCCCCCCCCC10.5235602094240838No Hit
CCCCCTTCCTACACTTGTAATTAAATATATATATATATATATATACAGAA10.5235602094240838No Hit
GACCCGCCCCCCCCCCCCCGCCCCCCCCCCCCCCCCCGCCCCCCCCCCCC10.5235602094240838No Hit
CGCGCCACCCCCCCCCCCCCCCCCCCCCCCACCGCCCCCCCCCCCCCCCC10.5235602094240838No Hit
CCGTATGATCCAGATATTTGCTGTACACGATTACAAAATCATAAGGAGTC10.5235602094240838No Hit
GGATCATCAACCTTGGCAAACTCTCACAATGAGCAAGTAATAAACCACGG10.5235602094240838No Hit
GCGGCTGGTACCAAGCTTAGTTGTGTATATTGTCGGCATGTTGCACGGGA10.5235602094240838No Hit
CTAGTCTATCTCATACAGAAATGAAAAGTTGGCCAATTTTACCGATTGAG10.5235602094240838No Hit
ATTTCACCTTGGAATTCAATTCCATGACGAAGGAGAACATATTACATTCG10.5235602094240838No Hit
AGCGGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCTCCC10.5235602094240838No Hit
CCCGGTTGTTCAAGGAGTTGCAGTAACATAAACTTCGCATCATGAACTTG10.5235602094240838No Hit
CCCCCACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCACCCCCCCC10.5235602094240838No Hit
GGCAAACATATATAGACATCATGACCAGGCAAAGAACAGTAACACCAAGC10.5235602094240838No Hit
CCCCCCGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCA10.5235602094240838No Hit
GTGAAGTGTCAATCAGAATCACATTACAGGTAATTATGCACAATCAACAC10.5235602094240838No Hit
CCCCCCGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC10.5235602094240838No Hit
ACCCCCGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCAACCCCCCCCCC10.5235602094240838No Hit
ATCATGTTATATCTGCAGTACTATACCTATGTTGGTTAACATACGTTGAT10.5235602094240838No Hit
ACGGGCGCACCCCACACCCACCCCCGCCACCCAGCACCACCCAACACGCC10.5235602094240838No Hit
CCCGCCGCCCCCCCCCCCCCCCCCCCACCCCCCCCCCCCCACGGCCCCCC10.5235602094240838No Hit
ACCGCCCCCCCCCCCAACCCCCCCCCCCCCACCCCCCCCCCCCCCCCCCC10.5235602094240838No Hit
GTAATCTTCTGTGGGCAATACTCCAGACTGCCATACTCTCTAACCAGACA10.5235602094240838No Hit
TCTAGTTATAAGAATACAAAACGGGAGAATAAAAGGCCCAATGCAAGTTG10.5235602094240838No Hit
ACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCACC10.5235602094240838No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCACCCC10.5235602094240838No Hit
CCACATCAGGTTATTATCGAGACGATAGAAGTGGAAGTCCCAGCCCGGTG10.5235602094240838No Hit
CCCCCCCCCCCCCCCCCCCCCCCCGCCCCCCCCCCCCCCCCCCCCCCCCC10.5235602094240838No Hit
AGATAGGATGTACATATACCGGTAGGTAAACATATTTTTCAACAATTTTG10.5235602094240838No Hit
TATTTTTAGAAAAATGTGTACTTTAACTATGGCAATGTTTTTTTTTTTTT10.5235602094240838No Hit
GTTCAATAATACTGATCCACTGTGTTCAATAATAATGATCTGCTGTGTTC10.5235602094240838No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCACCCCCCCCCCCCCCCCCCC10.5235602094240838No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCGCC10.5235602094240838No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCG10.5235602094240838No Hit
CAACTATATATTACTGGATTTTCTATGAACATATAGCATATAAATTTAGA10.5235602094240838No Hit
GTCTGACCTTCATGTGCAAATGCAAATATTAACGCGACGAGTTTGCTAAG10.5235602094240838No Hit
AATACTTATATTTTAATTCAAAAGAAAGACACAAAAAATAAAACACAATG10.5235602094240838No Hit
CAGGTTTGACGGCATGGTGATGTTATGACGGTCGTCTGCGAAATGTTACA10.5235602094240838No Hit
TTAAAGCTTTTATTAAGTGTTATTTTTTTAAAAGCACCGATTTCATTCCT10.5235602094240838No Hit
GTCATCGTGACTTGTCTCTATATGGCTCATACCCTTCCTCCTGGAACCTG10.5235602094240838No Hit
CCCCCGCCCCCCCCCCCCCCCCCCCCCCCCCCTCCCCCACGCCCCGCGCC10.5235602094240838No Hit
ACCCCCCCACCCCCCCCCTCCCCCCACCCCCCCCCCCCCCCCCCCCCCCC10.5235602094240838No Hit
AATCGGCATGCTCTACCATCAGCCCACTACAGTGACAGTGATTTCGCGCA10.5235602094240838No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCGCCCCCCCCCCCCCCCCCCC10.5235602094240838No Hit
ATTTCCTTAACAACTGGGCCCTGTCCATAGTAACAAGCCCAGTAGAAAAG10.5235602094240838No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCGCCCCCCC10.5235602094240838No Hit
GCATTTGTTACGATGTATTTTTCTAATGTACTTTTATATATGTATATAAA10.5235602094240838No Hit
CCCGCCCCCACCCCGACCCCCACACCCCTACCCCCCCACCCCACCCCCCA10.5235602094240838No Hit
ACACTGGAAAGACTGAAGTTTGAGTTAAACTGTGTAGAGTTACAATGTCC10.5235602094240838No Hit
ATATACAGGCCCCGTGTTCAACGAGGAACTTCACCATAGGAAGATTGTTA10.5235602094240838No Hit
ACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCGACCCCCCCCCCCCC10.5235602094240838No Hit

[OK]Adapter Content

Adapter graph