FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-gonad-RNA-5_S33_L005_R2_001.fastp-trim.20220908.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-gonad-RNA-5_S33_L005_R2_001.fastp-trim.20220908.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences196
Sequences flagged as poor quality0
Sequence length49-151
%GC44

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG136.63265306122449Illumina Single End PCR Primer 1 (100% over 50bp)
GGTAACGTGGACTACTCCTTCCGGTGGTAGTGGTGCCTTGGCAACGTCAG94.591836734693878No Hit
TTTATCCTTCTATATCAGTCCTTGATTTCATGCATAATTATGAGTAAATT73.571428571428571No Hit
AATTCATGTGGATGACTATGAAATATTATGCATATTTGTTACTTTATGTT63.061224489795918No Hit
ACCACACCCACATTCAGGAGCCCTGAGGGAAACCTCACTTTTGGCACAAC63.061224489795918No Hit
TGATATTTGCTGCCATATTAGTAGTTGTTTATATAATTTTAAAGGGTTGG52.5510204081632653No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT42.0408163265306123Illumina Single End PCR Primer 1 (100% over 50bp)
CCTTATATAACATAGGCTTCTATTTCACCAACATTTTTCGAAGTTTTACT42.0408163265306123No Hit
AGATCAATCATTCCATCATGTGACCAGTTTTGTACATCATCATTCCGTGT42.0408163265306123No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC42.0408163265306123No Hit
CACGGAACCACAATAGAACCAGAAAACAAGAAAACAAGTGCGGAAGAAAA31.530612244897959No Hit
CTGTAGCCCCAGCATCTGCAACTGTGACTCTTACGTTATCAGTTACAGCC21.0204081632653061No Hit
CCCCCCCCCCCCCCCTCCCCCCCCCCCCCCCCACCCCACCCCCCCCCCCC10.5102040816326531No Hit
GGTGAAGTAGCATTAAGTGTTTCCCCTATCCATCCTTTTGTTCGTTTTTC10.5102040816326531No Hit
GTACAAGACTTTACTGGCGACTTTGACGACACACTCGGAACGTTTATGGA10.5102040816326531No Hit
GTAAATGTGAAAGAACTGATTACCAATGTTGGAAGTTCAGCAGGATCTGC10.5102040816326531No Hit
GGGTACCCTGTACGCTATTAGTCGTGTACACTCTAACACCTGCATTGAAT10.5102040816326531No Hit
CCGGTAACACACAAAACAAGTGGAAAGAATCATGTTCGTCTTCGCAGTGT10.5102040816326531No Hit
TGTCACAAAAAGTCTTTTTGAAAAATATTTTATAATAGTCTACCAATGTC10.5102040816326531No Hit
GCACCGACTGCAGCTCCAGTTGCTGAAGAGAAGAAGGAGGTGAAGAAAGA10.5102040816326531No Hit
TTCCAATGGACAGTTAAACGACTACCAAGAGAAGAAAACGGCACCAGATG10.5102040816326531No Hit
CTGTGATATTGCATGTTTCTTTATACGTATACATATTTATTAATGTTTTA10.5102040816326531No Hit
CCCCCCACCACCCCCCCCCCCAACCCCACCCCCACCCCCCACCCCCCCCA10.5102040816326531No Hit
GCGACATTACCGTCTGTGAAGGCAAGGAAACCGTATTCACATGTGAGGTA10.5102040816326531No Hit
CAATCAACCAGTTTACTCAAGCACTTGACAGACAAACAGCCTCCCAGCTC10.5102040816326531No Hit
CCAGTGTATGCCTACAGATACTTTCTACACACCCATCATTACGGATATTT10.5102040816326531No Hit
GTCAGGACAAAGACCGTGAAGAAAGCTGCTCGTGTTATCATCGAGAAGTA10.5102040816326531No Hit
TGATATTGATCGCTGACTGGTCTTTCGATTAAAATCAAACTTGTCTTCTT10.5102040816326531No Hit
GTTTCTCTAAAGAAATCATATTAGACGATTTAGCCAAATACATGAAGTGC10.5102040816326531No Hit
CACAGAACTGCGTGTTTGTAATATAAAAACTATAGTATAACATGATTTTG10.5102040816326531No Hit
ATGACATTCTATGTATAGGTTTCAATAAACATAAGAGAGAAGACTGAGAG10.5102040816326531No Hit
TTTTGATAGTGTGGCTCTGAAATTCGCTCAGTATTTCTTTATCCTTTCAG10.5102040816326531No Hit
CCCCCCCCCCCTCCCCCCCACCCCCCACCCCCCCCCCCCCCCCCCCCACC10.5102040816326531No Hit
CATGCATCATTATGAATACTTCATGTGTGCGTCATCATGTATGGTGGCCC10.5102040816326531No Hit
CCGAATCTCAATCTTCCCTCTCAAGTTACCATGGTTGCTGTCATTTTCAC10.5102040816326531No Hit
GTGAATGACGGCACAAACGACGATCCACTGTCATTTGCCGCCATGAGTCT10.5102040816326531No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCACACCCCCCCCCCCC10.5102040816326531No Hit
GGACATGCAACAATATCTGGGTATTTGACCGTGACTTGGACGGAACAGAG10.5102040816326531No Hit
TCATTCTCGAGTGCATTCCCTTATTAGATCTGGTACCGATTTGTTTTTGA10.5102040816326531No Hit
AAAAATATAATATAAAAGGAGTAAGAACAAAAGCGTAAAGCTTATGCTAT10.5102040816326531No Hit
CATGCGGCCTCGCCATTGGACCAAAATACAGACAAAGATGCAACGTCAGT10.5102040816326531No Hit
CCCCCCCCCCCCCCCCCCCGCCGCCCCCCCCTCCCCCCCCACCCCCCCCC10.5102040816326531No Hit
TTACTACTGTATTTGTGAAGGCTGTGGGTGGTGAAGTTCCAGCTACTTCT10.5102040816326531No Hit
CTGCATTACCCATATAATACTCTATTTTCTTGGTTTATTTAATTAATATT10.5102040816326531No Hit
TGATACTTAACGGTGTGGTTAAAGTGATTGCGCCGAATGACGTGATTTGC10.5102040816326531No Hit
GGATGATCATCTAGCAATTAATTTATTATGGTCTTAACAACTACTTCAGT10.5102040816326531No Hit
GAGATAATACACGAGGATATCAATTAAAAAAAAGATTGCCTTGTCAGAAA10.5102040816326531No Hit
TTTGTGTTCGGTTTCGACGAGGGCATGATTGATGCATTAACAATGACCAA10.5102040816326531No Hit
ATTTACTTTGTTAAAAAAATAAATAAAAGATATAACTTTTTTGTATCTAT10.5102040816326531No Hit
CTGAAAACTTCTGTACCACTCAAGTCCACTATAAATCCTGTGCAAGTAAT10.5102040816326531No Hit
TTTGACTCCTGTAAAACCAAGAGGATAAAACATGACATTTTGCACCAGAT10.5102040816326531No Hit
CACAAAGGTTACAACTCCAAAAGTTGTTTCTGCAAAAAAAGGAGTAGCAG10.5102040816326531No Hit
CTGGGGTCTTCCTTCACCAATAAAACTAAACTGGATATGCCATATATAGC10.5102040816326531No Hit
GTCTTGCTCAAGGACACCACACATAGCAATGACATGTTTGTTTATTCCAA10.5102040816326531No Hit
TGAACATCCAGAGGAGGATATGATGTAAAGTATGTTTATCATAGCATCAC10.5102040816326531No Hit
CTCGTTTACCTGTGTTACTTAGCTAGATTTTCTCAACAACCTTGTTGGCT10.5102040816326531No Hit
CTTCCATGAACGAGATGGCAAGGCTTATTGCAAAGCTGATTTCTTCGAGA10.5102040816326531No Hit
AGATTGCGGACAGCTTTAATTAACTGAAGACTCATGATATTAAATTGTTG10.5102040816326531No Hit
GTGAGTCTGTCACACAGTTGGTGCGAGTACAATCCTGCTTCTAGATGCTT10.5102040816326531No Hit
CCCCCCCCCACTCCCCCACCCCCCCCCCACCCCCCCCCCCCCCCACCCCC10.5102040816326531No Hit
TTCATGGGTTGCATGTTTTAATTGGCACTCTATTTTTACTAGTTGGGTTA10.5102040816326531No Hit
AACAGGATACCTTCATTTTTTTCTATATGGGCAACGTTTATATTTCAACA10.5102040816326531No Hit
GTCGAAACTCGTTATCTCGATATTGGAGGGACTGCCAAAAAAACTCGAGA10.5102040816326531No Hit
GTTAATTCAATAATTCTAAATAACAAGTTCTGTACTCAACCACTTTGTTT10.5102040816326531No Hit
CAATGTGTTATTTTAGGGGGAGACGGTTTACAGCCCCTGAACATTGGAAC10.5102040816326531No Hit
TGACCTTCCAAATTGCACCTGCAATGATTATAGCTACACGTGCCAAGGAG10.5102040816326531No Hit
TGTACATGCATATTTTAGAGGGGGAAATGACAAAGTTTTTTTACTTATTT10.5102040816326531No Hit
ACTCAGGACACTTCATCTGCATGTACACAGCACTGACTCTACTCAGGACG10.5102040816326531No Hit
AAACAAACAACAAAAAAGCCTACAGATGAGAACTCTAATAAGGAAAGCGA10.5102040816326531No Hit
CATTACTCCGCTAATCTCGCCATCAATAAACCAGTCTTATCCATCACTGG10.5102040816326531No Hit
CTTGAGCTCACTTAACAATCGGGGGAAAATATATTAGCATAAATGAATTA10.5102040816326531No Hit
CTGGAATAGGTTTTAATCTGATGGAGGGTCCAGATTTCTGACCACAAAAC10.5102040816326531No Hit
CCCACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCGCCCCCCCCCCAC10.5102040816326531No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCACCCCCACCCCCCCGCCCCC10.5102040816326531No Hit
GGCACATTGCTCTCTTCATTGTGGATTCTGTGATGGGCCATCTACAACAC10.5102040816326531No Hit
CCCCCCCCCCCCCCCCCCCCCCAACACCCCCCCCCCCTCCCCCCCCCCCC10.5102040816326531No Hit
ATGATACTTTCTGTTTAAAATACAAGCAATGCCAAGAACTGTTGTTTTTT10.5102040816326531No Hit
TATGGCCCTTTTTGAACTTAGACACCCAGGTTTAACTTTTGCGTTTAGTT10.5102040816326531No Hit
TCTGAACCAGCTGTGCAGGCCCGTCTGGAGAGTCTGGCATCCCAGTGGGA10.5102040816326531No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCACCCCCCCACCCCCC10.5102040816326531No Hit
CCCCCACCCCACCCCCCCCCCCCCCCCCCCCCCCCCCCCACCCCCCCCCC10.5102040816326531No Hit
GCCTTTTTTACCATCTAGGGCCATGTCCGACTCCGAAGACCAACCGGAGA10.5102040816326531No Hit
TTGAATGCCGTGTATACAAATATTAGTAACGACGATAAATGTTGGAGCAT10.5102040816326531No Hit
CCCCCCCCCACCCCCCCCACCCCCCCCCCCCCCCCCCCCCCCCCCCCCAC10.5102040816326531No Hit
TGACATTTGAGGGATGGTGACATCATGTAATGAGCCCGTGCAATACTGCG10.5102040816326531No Hit
CATGCGCGCTGTTCTGTGTTTAGCTCTTATTGTCGCCTGCACCGCGCAGG10.5102040816326531No Hit
CCGGCATCGTCTATAGGCACCCGGGTTAGACTTTTGCGTTTAAGTTTAAG10.5102040816326531No Hit
GCTTCAGATATTGAGAAGATCCTCACAAGTGTTGGTATAGATGCTGAAGG10.5102040816326531No Hit
CGGTTTTCGGGCAGCATACCAGTCAAACCCACTCTGGCAGTATCATCTCA10.5102040816326531No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCTCCCCCCC10.5102040816326531No Hit
CCCCCCCCACCACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCAC10.5102040816326531No Hit
CTCTCATCTGCGAAGTCACTTCTGTCTTTCTACATTCCCATTGTATTTTT10.5102040816326531No Hit
ATTTTTAATGCAAGCTGGATTTGTTTTCTACCAATTTGTAACAAAACAAT10.5102040816326531No Hit
AAGCTTGCTAGTTAGTTGTATTTTTTTTTTACGTTTTTCAATTCTCGCAT10.5102040816326531No Hit
ACTTACACAAATGATAGGATACCCGACGATGGGAATCCGTCGTTAACTAA10.5102040816326531No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCAC10.5102040816326531No Hit
TCTGCTTCGACTGGGAAACAAGTTCTTATCACTTAGGCAAATTACGTTCG10.5102040816326531No Hit
GTTCCCGCAGTCCCTCAGCGGGAGGGAAGCATGGGAGATTATCTTGTGTG10.5102040816326531No Hit
TCTGGAGTCACGGCTACGCGAGTTCAGTAAAAATGACATAGCCATCCCAG10.5102040816326531No Hit
TATGTATAGTCGGTGTTGCTCCCAACGAAGCTTTTGTATTGCTAGATATA10.5102040816326531No Hit
TTCAGTATTACCTTGTGAGATTACTTACTTTGTGCCTTTTAAAGTTGACA10.5102040816326531No Hit
CTCGCTTGTGTTGTTGAATGCTGTCGACACGGTGAAAAAGATTGGCGACA10.5102040816326531No Hit
AATGCATAACAGATGACTGTCAACAAATATTCTTATTTCACAAGTTCCAG10.5102040816326531No Hit
ACTTGTTCTACGTATCCCGTGCACTCGACCAAGTGGCCCGGCCAACCGGT10.5102040816326531No Hit
ATATTTACATTTTCTATACCTCATTTTTTTTTAGATTTAGTTGCAAGTAC10.5102040816326531No Hit
AAAATTCAAGATTTAAAACATTTAAGAAAAACTTAAGAGAATGTTGATGA10.5102040816326531No Hit
ATGACCTACATGCACAGATTGAACATGTTTCAAAGAACAGGGGTATGTCC10.5102040816326531No Hit
CTGGAGTCCTGATTCACAATGCATTGCTACTGGTAGTCTTGATGCATCAA10.5102040816326531No Hit
TTTTTAATGTTCTTAGGAGTTAATTTAACGTTTTTTCCTCAGCATTTTTT10.5102040816326531No Hit
TGTTCTTGAGAAGCCGAAAGAAGGCGAAAAAAAGTACTCCAATATTATGG10.5102040816326531No Hit
GCACAACTTGCCATTTAATTTCAGGTATTCTTAATGTACATCTTCTATTG10.5102040816326531No Hit
TGATTTCTTGATGCAGATATGACATTAAAAATCCATGTTTGAATTTTTGC10.5102040816326531No Hit
CCCCCACACCCCACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC10.5102040816326531No Hit
CACACACTCAATTCCACTGATTTGCCACTCGTTTCAAGAACTTGTACAAC10.5102040816326531No Hit
CCCCCCCCCCACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC10.5102040816326531No Hit
CCGGTTTCCTCAGCACAGATACGGTGACAATTGGTAGTATAGTTATCAAA10.5102040816326531No Hit
AGGAAGTTGACGTCATCATCACAACTGCATTGATTCCTGGCAAGAAAGCT10.5102040816326531No Hit
CTATATTATTGAGGAGCGCGTGGTCCAGTGGTAGAGCTCCGGACCCATGA10.5102040816326531No Hit
TTAGCTACTATGTCGTAAAAAGAAATTTAAAAATTTAGGGATTATTTTTT10.5102040816326531No Hit
ATCAGATTGTCAGCAGGGTAATAATGCAGGGAACATACACTCAAATAGTC10.5102040816326531No Hit
ACCCCCCCCCCCCCCCCCCCCACCCCACCCCCCCCCCCACCCCCCACCCC10.5102040816326531No Hit
CTGACACACAGGAGGTTAATGAACCTCAACTCCATGACATCTCAGTCATT10.5102040816326531No Hit
TGATACGGCATACGAGATATTGGCGTGACTGGAGTTCAGACGTGTGCTC10.5102040816326531TruSeq Adapter, Index 6 (97% over 47bp)
GGTAGTTACTGGACACTCCACCCGGACTCCGGCAACATGTTCGAAAATGG10.5102040816326531No Hit
TGCAGTTCAAAATGGGTAACTGCCTCACGCAAAAGTTGATTAACGAATTC10.5102040816326531No Hit
CAATGGGCTATCGATCATCAAAATACAACCCACCAGAGTGGCATGAAAAT10.5102040816326531No Hit
TTTGTTTCAAGTTCAAGAACTTTTTAAAACAATTTCATTCACAGAACATG10.5102040816326531No Hit
GGTCATTTGGCAGTCCTCAAAACACTAGAGATGAAGAAATGAGGAAAATC10.5102040816326531No Hit
AGACTGAAGATACGGCTTGAGAAGATGCTGACGAAAAGGTTGATATATAA10.5102040816326531No Hit
GGGAATTCCAGAGAGAGGAATGCGAGCCGACTTACACCCACACCACCCCA10.5102040816326531No Hit
TATAAATGGTGTACATCCCCGACCTAGAAAAATTATGCAGCTGTTCCGAC10.5102040816326531No Hit
TCCATAACTTCTGTTTTATTAAACCATTACATGTTCATCATTGTTTTTAG10.5102040816326531No Hit
TGAAAATTAAAATGACTGGGTCAGTGATTCAAGTATTGCTTTAAGGACAA10.5102040816326531No Hit
GTGATAGATGTCTCACTTATTGCCCATGGCATACAAATGTCATCAAGTAA10.5102040816326531No Hit
CATCCGGCAAGGTTTTAGCACCTCTATATACACCGATGTGGACCTGTCAG10.5102040816326531No Hit
AATTGTGCCAATCAGAATTAACCAGTGATAACAGTGACATGCAGGATCAC10.5102040816326531No Hit
AAAAAACAGTCATTGGGAAGTTTCACGCAGAAATTAGATGCTAAGCTTCT10.5102040816326531No Hit
CCTCTTCCACCACCCCCCCCCCCCACTCCCCCCCCCCCCCCCCTCACCCC10.5102040816326531No Hit
GGAACCGCTAAATAAAGTTGACGTCAGGTGAGAGCATGAGAGTAGCTTCC10.5102040816326531No Hit
TTTTTGTGATTTTTTTTTTTTTTTTTGTATATTTGATTTTAACGTAAAGA10.5102040816326531No Hit
CAAGACATCACATATATACATGATGTAGTAAGTTTCTGAAACTTCAGTTT10.5102040816326531No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)