FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-gonad-RNA-5_S33_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-gonad-RNA-5_S33_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1073
Sequences flagged as poor quality0
Sequence length49-151
%GC62

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC151.3979496738117427No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC141.30475302889096TruSeq Adapter, Index 6 (100% over 50bp)
GAGCTGTCCAACTGTAGTACCTGTTATATCAAATTTGGAACCGGGATTCG90.8387698042870456No Hit
GTCGACACAGGCATGATATATTGGAAAAAAACATGTTTTTAGCCAATATT70.65237651444548No Hit
CTCCCCTCCCCTCCCCTAAGGGCACAGACCATTGGACCACCACACCCAGC50.4659832246039142No Hit
ACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC50.4659832246039142No Hit
AGAAAAAAAAAAAAGCTAATTTATTAATGTACTAAGTTTAATCGGACAAA40.3727865796831314No Hit
TTTATGAAGGCCATTCACTATTGTTCACCCTTTTTTTAACAATGAAGCAA40.3727865796831314No Hit
GCCTTTTCGTATTTTTATAATCCCTACCGGGGAGGGTGTTTTTCCAACAG30.27958993476234856No Hit
CTGACATATGGGACAGTGTTCTTCACGAGACTTTATCTCCTCCGCAGTAG30.27958993476234856No Hit
TTAAAATAAAAATTAATCAAACCTGGCTCTTATTTCAGCAACATGATTAC20.1863932898415657No Hit
GTGTTACTCCCGGCCGATATACTGTAAGTAAGAGTGTCACTATTCGGATC20.1863932898415657No Hit
CAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA20.1863932898415657TruSeq Adapter, Index 18 (96% over 29bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTAATATCTCGTATGCC20.1863932898415657TruSeq Adapter, Index 6 (98% over 50bp)
TTTCTGAAGGCCATTCACTATTGTTCACCCTTTTTTTAACAATGAAGCAA20.1863932898415657No Hit
CTGCCATATGGGACAGTGTTCTTCACGAGACTTTATCTCCTCCGCAGTAG20.1863932898415657No Hit
CTCATCTGTAGCATAGCCAAACATCAAACCCTGATCACCAGCACCAATAT20.1863932898415657No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)