FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-gonad-RNA-5_S33_L005_R1_001.fastp-trim.20220908.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-gonad-RNA-5_S33_L005_R1_001.fastp-trim.20220908.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences196
Sequences flagged as poor quality0
Sequence length49-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC126.122448979591836TruSeq Adapter, Index 6 (100% over 50bp)
GAGCTGTCCAACTGTAGTACCTGTTATATCAAATTTGGAACCGGGATTCG94.591836734693878No Hit
GTCGACACAGGCATGATATATTGGAAAAAAACATGTTTTTAGCCAATATT73.571428571428571No Hit
CTCCCCTCCCCTCCCCTAAGGGCACAGACCATTGGACCACCACACCCAGC52.5510204081632653No Hit
TTTATGAAGGCCATTCACTATTGTTCACCCTTTTTTTAACAATGAAGCAA42.0408163265306123No Hit
AGAAAAAAAAAAAAGCTAATTTATTAATGTACTAAGTTTAATCGGACAAA42.0408163265306123No Hit
GCCTTTTCGTATTTTTATAATCCCTACCGGGGAGGGTGTTTTTCCAACAG31.530612244897959No Hit
CTGACATATGGGACAGTGTTCTTCACGAGACTTTATCTCCTCCGCAGTAG31.530612244897959No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC31.530612244897959No Hit
CTGCCATATGGGACAGTGTTCTTCACGAGACTTTATCTCCTCCGCAGTAG21.0204081632653061No Hit
TTAAAATAAAAATTAATCAAACCTGGCTCTTATTTCAGCAACATGATTAC21.0204081632653061No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTAATATCTCGTATGCC21.0204081632653061TruSeq Adapter, Index 6 (98% over 50bp)
TTTCTGAAGGCCATTCACTATTGTTCACCCTTTTTTTAACAATGAAGCAA21.0204081632653061No Hit
GTGTTACTCCCGGCCGATATACTGTAAGTAAGAGTGTCACTATTCGGATC21.0204081632653061No Hit
ATAAATGTGGTAAAACCTTGCCGGATGACATAGTCATACCGGTGTATATA10.5102040816326531No Hit
TCAATTTTAATCCAACAATTTCATCACTTTAAAAACACATATTTAACAAA10.5102040816326531No Hit
ACCCCCGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCACCCGCCC10.5102040816326531No Hit
CACAATCCATAGTAAAGCATAACATAAATTAAGATATAACGTCTCTGAAA10.5102040816326531No Hit
AAAAAGTATTTTTTCTCATACCTCTGACATGACTGTGTTTGTAAAGACTG10.5102040816326531No Hit
GCACGTTCATATGTGAATATATTTTTATTTACATTAGGATAATCCATAAA10.5102040816326531No Hit
TAAGAATATTTGTGGACAGTCATCTGTTATGCATTATAATGTTGCTGGAT10.5102040816326531No Hit
CCATCAATAAACAAAGATAAACAAAAAAATCCAAACTACGTCGACAAAAT10.5102040816326531No Hit
TTTCAATAATGGCGTTGTCTGTGAGTGGGACCCTTCTGCTGTGGACCTTA10.5102040816326531No Hit
GTAGGACACCATTACCGCAAAATCCACAGTATTTTGGACAGTTGTTCTTT10.5102040816326531No Hit
GGGGGTTGTTCTTGATTATGGCAGTCTGATGTACAAAAACGTCTTCTTTG10.5102040816326531No Hit
TGATATTTAACATTAATACACTATAATTCAAACAATAGGCCCATGGGCCT10.5102040816326531No Hit
GCCAGCTTAACCTATCAACGAGTGCAGAGAAAACCCAGTGAAATCTTTGC10.5102040816326531No Hit
CAAAATTTGCGAGGTTTCCTCTTTAACTTTTATAATTCCAATGTTATCAT10.5102040816326531No Hit
TGGAGCGGTCAAGTTCTTAGATAGACTAGAACAATATCACTGAGCAGGGT10.5102040816326531No Hit
CGACGCAGTAAATAAATTAAAACTTTTAGAGAAAACTACTTAAACCAAAT10.5102040816326531No Hit
GTAGAATCAGTGTTGTGTACATGCAGATGGAGTGTCCTGAGTAGAGTCAG10.5102040816326531No Hit
GTAGTGACTGTGTTGGTACCTGCTCGTACAACTGGTGGACGCTTTGTTGG10.5102040816326531No Hit
TCTAACAGTAAGTTTCTCTCCCCTAGATATGAAAACTTTTCTCTACATTT10.5102040816326531No Hit
TAGGCACTTTAAATGAATGTCCGCGTTATAGACATTCGTGTATCTTTTGA10.5102040816326531No Hit
CTGCCATCTGCAAGGTTAACTTCAATATTAAGTAAATCATCAACTAAGCT10.5102040816326531No Hit
CTCAAATTTTGTGTTTTATGTCACACTGACTGTTTAATTGGCAGTCATCA10.5102040816326531No Hit
AAACAATTTATTTTTTAAGGACGGCCTAAAGTATACTTATATTATAGTAT10.5102040816326531No Hit
CCAGTACAAAGCATATACGGAAAAGTGTCTTGCTCAAGGACACCACACAT10.5102040816326531No Hit
TGATCCCCGTCCTTGAGCATGCTTTTTGTTCTTGAGCCAGGAACCATGTG10.5102040816326531No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCACCCCCCCCCCCCCCCCCCACCCC10.5102040816326531No Hit
TAGGGCATTAACAGCATCATCAAGTAGAAATCTCTTATTTGCCACTGTAG10.5102040816326531No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC10.5102040816326531TruSeq Adapter, Index 6 (100% over 50bp)
CAAAATAAAAACAACAATGTCAACTTTCAATATAGCTTTCTTAATTACTA10.5102040816326531No Hit
CCCCCCGCCCCCCCCCCCCCCCCCCCCCCCACCCCCCCCCCCCCCCCCCC10.5102040816326531No Hit
ATTTCAATGGCCAATTCCTTGGTAGCTGCACCAGACACCTCATTGATGGA10.5102040816326531No Hit
TGATAATGTTACAGCACTAAATATCTGAGCACTTTCATAGAAAGTTGCAC10.5102040816326531No Hit
ACCCCCCCCCCCCCCCCCCCCCCCCCACCCCCACCCCCCCACCCCCCCCC10.5102040816326531No Hit
ACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC10.5102040816326531No Hit
CCATAACCATATTTCTTCATGTGACACGATTTATTTAAAATGGATCAATG10.5102040816326531No Hit
AGACAGGCTTGACTGTATAACAAAATAAGGAATAGCATATGCTATACTGT10.5102040816326531No Hit
TAATGGCCCACCTGGTATGTCAAACATATGCTTTGATTTCTCCATACTGA10.5102040816326531No Hit
TTTCGATAAAAGCATGAAAGCTTTCCATCAGATATTCCTCAATATTTCTT10.5102040816326531No Hit
NATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC10.5102040816326531TruSeq Adapter, Index 6 (98% over 50bp)
TGACTGGCTGCTTAAGGCAGGTTTTACTGTATTGCAACTGCCGTTTGGGA10.5102040816326531No Hit
GTATTCCTTATGCTCGTTGTAGAGATGTAACCTGTCGTAGAAGTCCCTTT10.5102040816326531No Hit
CTGCAATTAAGGTTATCTGTGGCAATGTGAAGTGGGATTTCTGTAGCACG10.5102040816326531No Hit
CATGAATTTTATCTCAGCCCTTGAGTATAGATATAGAAATATCTAACACC10.5102040816326531No Hit
AAACAATTATTTTTTCTTTCAAGGCTTTAGTTATTAAATAATAACAGGAC10.5102040816326531No Hit
AGAGTGTTTCACATGTTTGACCTTCTTTCTGTCCCTTGGGGGCTCCTTAA10.5102040816326531No Hit
ACAACATGCTGCAATTATGAGTTTTCAGTAAAAAAATGAGGTTCGGCAAA10.5102040816326531No Hit
ATCCAACCTGTCAGTGTGAACGGTCAGCCCTTTATCCACTAAACTACCGC10.5102040816326531No Hit
CTCAAATCTAATCAATTTAAAACATTTGATAACGCTCCATATATGAGCAT10.5102040816326531No Hit
ACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCACCCCCCCCCCC10.5102040816326531No Hit
GCCAGTTACAAAATCAGTATATGGGAAGGCATGAAACTGTTAAAATGGCC10.5102040816326531No Hit
ATTAACTGTCTGAATGTCAAATGTACAATTGAGACACTTTTGCACTCAAT10.5102040816326531No Hit
AGAATTCACATTTTCAATATAAACTGCATGTACTCGTTAATTAAGAGTAT10.5102040816326531No Hit
GCTGTGTACTCAAAGTTACATGTTTGTCGATCAAACGGATAGTATGTGAC10.5102040816326531No Hit
TTTATTGCTAAAAATGTCGAATGCTTCATCTGGAAGTCTGTTGAGTACCT10.5102040816326531No Hit
TTCCATACTGAAGTCCCGGTTTTAAAATTATAGTTCATGTTATCTGTTGT10.5102040816326531No Hit
CTTCTGTCGTCTTTCCACCGAGGATTTCTTCTTCCTCTCCCTTTTCATCA10.5102040816326531No Hit
TTAAAGTAACATTTTCTTAAATTTCCATTTACAGGTTTAAGTTAGAAATT10.5102040816326531No Hit
ACCCCCGCCCCACCCCCACCCCCCCCCACACCCCACCCCCCCCCCCCCCC10.5102040816326531No Hit
GAAAAATTGAAATTTGAGGAATAAAGTCCAAAAAGGGCCATAACTGTGCC10.5102040816326531No Hit
ACCAAATGTTTGATGGTTATGATTTAGCAAACTATGTTAAAATGTAAAAT10.5102040816326531No Hit
TTCCTTTTGTATTGTACTCATTATAAATTTTATGCTACAAATAGATGTTT10.5102040816326531No Hit
TGAAAACTGGTAATCACAGGGGAATACTTAAATTACCTGTATAACTAGAG10.5102040816326531No Hit
ACCCCCGCCCCCCCCCCCCCCCCCCCCCCACCCCCCCCCCCCCCCCCCCC10.5102040816326531No Hit
GGTTCTTGTCTCTGTATGTTCCATTGATGATGGTGAACACACATGCAAAG10.5102040816326531No Hit
TAGTGCCCCACTTGTTTAAAGCATTGTATTCCTTAACTCCATTTTAAGAT10.5102040816326531No Hit
TTTTTTTGCACATCAAATCATTTAATGCAGAGAAAGCTGTCCATACACAA10.5102040816326531No Hit
TGTATATATAGCATTTCTTGTAAATCGTTCAATAGCATTTTTGGCAAATC10.5102040816326531No Hit
ACGACCCCCCCCCCCCCACCCCCCCCCCCACCCCCCCCCCCCCCCACCCC10.5102040816326531No Hit
CAGGGTGTGAAATATACTGAATGTATACAAAATTTTAACGAAGTACAGAA10.5102040816326531No Hit
CACATGTATACATTGCACAAAACCAGTCTCTATGGCAACACATGTACAAA10.5102040816326531No Hit
TAAAGTGCTGCTAACACACTTTGGAGACGACAGACGATGGACAATGCACC10.5102040816326531No Hit
CATATCCAGCTTAACTGGTGGAGGAAGACTTCAGACTGAGTAGAACCACC10.5102040816326531No Hit
TAAACAGGCAACGAAAATGAGAAAATAAGTACACAAAAAGCAAATGATTA10.5102040816326531No Hit
TGAAAATGCAAACATTTTATATTTATATATTATCTTCATTTTAGTAATGC10.5102040816326531No Hit
TAATAACGGAATTTACAATTCTTATCAAACTATGGACTGAAATGAGAATA10.5102040816326531No Hit
CTTTTGAAATGCCCCTCGCACATATTCACTGATTCTGTTTGAACTACATC10.5102040816326531No Hit
CTCACCGTTTGCGTTCATTAACAAAGTAAACATGTTTAACCGTGCTGAAC10.5102040816326531No Hit
GACAATCTGATTGTTTGTTCCCCGTATGATTCCATCGCTGACTATTTGAG10.5102040816326531No Hit
AAAGCTACAAATGAAACTAAAGACCCTCATCTAGAAACTATATTATAAAA10.5102040816326531No Hit
GGAACTGTCTTTGCACGTGGCGGCATCCATGAAACATTTGTTGGAGTATG10.5102040816326531No Hit
TTCAATAATTTTAGCGAAGATTTCCTCGGCAATAAAGCTTCCCCTCCAAC10.5102040816326531No Hit
GGGCTGTCAAACAAGTCCATTATCTAATCAACAATTGATCATGGTTGTTA10.5102040816326531No Hit
ATCGGAAGAGCACACGTCTGAACTCACAGTTCCGTATCTCGTATGCCGTC10.5102040816326531Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
ATTCTGATACATTATCATGGCCAACTTTATTCATACACCTTGACTTTTCT10.5102040816326531No Hit
TTTTCTCTTCTTTAACCTCACCACCAGCTGCTGGTGCACTAGCTGCACTG10.5102040816326531No Hit
TTACAATAAAAATTAATCAAACCTGGCTCTTATTTCAGCAACATGATTAC10.5102040816326531No Hit
GTCAAGAGCTAGTTGGTGCTGTTTTTCAAGAGTGAATTTTGACCATTCGA10.5102040816326531No Hit
CTCTTCCTCCTGCAGCTTGATGGAGATACCACGGACTGGTCCCCTCTGGA10.5102040816326531No Hit
CCCCCCGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC10.5102040816326531No Hit
CTTTCCCTTCCCTCTTGATTTGGTCTTCCGTAAACTTCTCTTGGCTAACA10.5102040816326531No Hit
AAACCTCAAAAGAAAATTAAATCCACTGTAATCGTGCAATTACTAAAAAT10.5102040816326531No Hit
CTCCACAATATCAATATACTCCATCCTGTTCCTTTTATCACCACTTATAG10.5102040816326531No Hit
CGAACTCGTTTTCGAGATTCGTGTGTTTCTTCTGGAGTGCCGAAAGGTCT10.5102040816326531No Hit
TTTCAAGGAAATTGTTTTGATATTTTTTGAGAAAATACATAAAATGTCAT10.5102040816326531No Hit
CAGAATTCTATATCCTTGACACCAGCATTGTAAGTCTGTTGCCTGCTTGC10.5102040816326531No Hit
GAATAGTTAGATTGATTTATCACTCAACGTGATTATGATTTTAACACGTC10.5102040816326531No Hit
ACCCCCCCCCCCCCCCCACCCCCCCCCCCCCCCCCCCCCCACCCCCCCCC10.5102040816326531No Hit
ATAAAGTGGGTCATTAGTACAGAGAATGAAGTGTTGGCTTCTGACGCTAG10.5102040816326531No Hit
GTCAGAGTTATCGTTAGCCAAACGTGCCTTTGATGAATTGAGCTCTGCGA10.5102040816326531No Hit
AACAAAACAAATTTTAATAAATCTCCGAAAGTCAGTTTACAATAATTATT10.5102040816326531No Hit
GAAACACTGTGCAGCTTTCACTGGCTCATCAAAATGTAGATAGCTGAGTG10.5102040816326531No Hit
ACCCCCGCCCCCCCCCCCCCCCCCCCCCCCACGCCCCCCCCACCCCCCCA10.5102040816326531No Hit
ACCCCCGCCCCCCCCCCCCCCCCCCCCCCCCCCCCACCCCCACCCCCGCC10.5102040816326531No Hit
TGACACTTCAGGGCTGCAATATTCCTGTTATACGTCTCCTCCGATTCTAT10.5102040816326531No Hit
GCCATCTGCCAAATCCTTGTCTAGGTTACTTGCTCGCAGGTGTTCACATG10.5102040816326531No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCACCCCCCCCCCCCCCCCCCC10.5102040816326531No Hit
CAGCAGTACCTATATATGTATATACAAACTAAAGACATCCCCGACAAGAC10.5102040816326531No Hit
AAATACGTTAATCACAAAATTATCAACTTCACTATATTTATGCACTCCAA10.5102040816326531No Hit
CCCCCCTCCCCTCCCCTAAGGGCACAGACCATTGGACCACCACACCCAGC10.5102040816326531No Hit
TTATTTTAATAAAAATCATGTTTCAAACACGCATAGTACAACTACACGAC10.5102040816326531No Hit
TGAAGCCGTATTCTCTATAATATTGACTGTAGTAACTGTGGGAGTTGTCA10.5102040816326531No Hit
CTTTTATTGAATCCATTTAATTAAATTTCTTTTTTAGTTCGACTGTTGAA10.5102040816326531No Hit
GATTTATTCATTGAACTTGAATTTCGATATAATAATGTTGTTTTATGATA10.5102040816326531No Hit
TTTTACTTTATTTTTTTTTTTTAAAATGGCATTTCAAGCTGTTTGTTTTA10.5102040816326531No Hit
GCTGGACCTGATACTACAGACAGCCCTGGCCCGTGCTGGGAAGGAAATCG10.5102040816326531No Hit
AAGCTGGTGGTCTCTCTTCGTTGCATGCTGGGCCGGCCTCATCATCCTTC10.5102040816326531No Hit
CCGGAATTGAACACAATGTCGTGTAGGCATATAATTTTCAATTATTAAAC10.5102040816326531No Hit
CCCGCCCCCCCCACCCCCCCCCCCCCCCCCCCCCCACCACCCCCACCCCC10.5102040816326531No Hit
TCAAAATAAAAATTAATCAAACCTGGCTCTTATTTCAGCAACATGATTAC10.5102040816326531No Hit
TAACAATAATGCATATACCAAAAATAAGGTCCCATTTATGAAATCTGTGT10.5102040816326531No Hit
AAGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC10.5102040816326531No Hit
GTCAAGTATACCCAGTACTGTGTCAAGTATACCAAGTACGGCATCAATTT10.5102040816326531No Hit
CAAGCCAGAAGGAGAATACAGCAGGTTTGACTAATCCTTCCATGACAGCA10.5102040816326531No Hit
TCCTATACAAAATGATCGATCCAATACAACGACTGTCAAATGAAACATCT10.5102040816326531No Hit
GGGGTATGTTACTCCATTGGTTGCACAAATCTTCTCATTGATGGTGTATG10.5102040816326531No Hit
TACACATGACATATCGAACAACCTTCGTATAGCTCTGACAAAATAACCTT10.5102040816326531No Hit
GGCAGACCTTCGTAGTCGAAAAAACTTCCACCATTGAACGGTTGATGACA10.5102040816326531No Hit
CCCCCCCCGCCCCCCACCCCCCCCCCCCCGCCCCCCCCCCACCCCCCACC10.5102040816326531No Hit
GGGAGGTCTGTGTACCCGATGTGAGTGACATCGTTGTATTTGTACAGCTC10.5102040816326531No Hit
CCCCCCGACCCACCCCCCGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCA10.5102040816326531No Hit
CTGTATTCCATACAATATGCTGATGGGAATGGGAAAATCTGTAAATGTCA10.5102040816326531No Hit
CATCAGTTTAACATTACAAAATTCCCGAGTAAAATTAGTTTAACAGTACA10.5102040816326531No Hit
GAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTATCA10.5102040816326531TruSeq Adapter, Index 6 (97% over 47bp)
TCCAGTTGCCCGGACATATGATGCTGTGAAGGATGACTTTGGTGTGACAC10.5102040816326531No Hit
CAGTTTTCTTGATGATCAGTGTGTATGTGTTTCCCTCGTGTTTAACACTG10.5102040816326531No Hit
AAAAATAACAACTGAACTATTTATATGCATATATCTTTGTTACATTTCGT10.5102040816326531No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)