FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-gonad-RNA-3_S17_L003_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-gonad-RNA-3_S17_L003_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1104
Sequences flagged as poor quality0
Sequence length68-151
%GC62

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC161.4492753623188406No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG151.358695652173913Illumina Single End PCR Primer 1 (100% over 50bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT60.5434782608695652Illumina Single End PCR Primer 1 (100% over 50bp)
TAAAAATGACTGGGTTCCACTGTCTTAAGGGGTCAGGAGGGGCCCAAAAG40.36231884057971014No Hit
CTTCTCTATAAAGGAGCCCTGAAAAGTAGTTTTGTCCATCTGGTTGTTGT40.36231884057971014No Hit
GTCATCTTGGACATTCAACAATCCACCGTCGTGGCGTATGTTTACAAACT30.2717391304347826No Hit
AGCCATCCCAGTCTTTAACTCCTCGACCTGACTTTTAACAAACAGTTACA30.2717391304347826No Hit
GTGGAGGTGGCGACTCCGTGATTGGAATCAGTGGACATCTGGGAAATCAA30.2717391304347826No Hit
ATTTGGTCCCTGGTGTCTGAACAGACCCGTGAAAAGTATGCTAAGGAGAC30.2717391304347826No Hit
TGGATATCAATATTTTTTTTCTATTATCTCTTATTTTGATACTCCTGTTT20.18115942028985507No Hit
GGTACAGCAACAATCCGCACGCGCAAGTTCATGACGAATCGTCTGCTTTG20.18115942028985507No Hit
CTACGTCTTGTAATGCTGGCCTGTCATTAAAGTTTACAAATTGTTTGAAT20.18115942028985507No Hit
CGGCAACCAGACTCAGTCAAATTTGTGACCATTATTCAAAAACTGTGGAC20.18115942028985507No Hit
TTTTGGCCATTTTTAATAGTTTTGAAAAGAAATGATATACTTTCCCTCTA20.18115942028985507No Hit
CTGGATTAACAATGACCTTACTATAGCCATGGTTGGTGCACCGGTAGATC20.18115942028985507No Hit
CCCCCCCCCCCCCCCCCCCACCCCCCCCCCCCCCCCCCCCCCCCCCCCCC20.18115942028985507No Hit
CTCTGATTCAGTCTGAGGTCTGCCTCCACCAGTTAAAAACTAGTTTAAAT20.18115942028985507No Hit
GAAACTCGTGCTGTATTAACAATAAACATGGATAGTAACACGATATCAGT20.18115942028985507No Hit
ATTCAACCAGAGTGGTATTTCTTATTTGCTTACACGATCTTGCGGAGTAT20.18115942028985507No Hit
CAAAGAGATACCTGAAGTACCTGACCAAGAAGTACTTGAAGAAGAACAAC20.18115942028985507No Hit
GAGCGAAACTGTTTGTTACCTTCTTCTTTCTTCGATATCAGCTGTTTCGC20.18115942028985507No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.18115942028985507No Hit
GGAAAATATGGCGGCTATTATTAAGAGTGATGCGTAAAATGAATACTCGA20.18115942028985507No Hit
AAAGCCCTTCCCCTGGGGTGACGGGAACCATTCCCTTCTGCACAACAGCC20.18115942028985507No Hit
CCTACATCAAAAAGATTGGTTACAATCCCAAAGCTGTTCCATTTGTACCA20.18115942028985507No Hit
GGCCGTTAAACAGTAACACCTTTTTGTCTCTGAATAAACCGCACTCACGA20.18115942028985507No Hit
GACCCAGGTCGGAATCCTGTGACAAGTATCTTTTTTTCCCTAGCAACTAA20.18115942028985507No Hit
AATTTTTACATTTTGTTCAACACATTGACATATTGCAACACTGATCTCAT20.18115942028985507No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)