FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-gonad-RNA-3_S17_L003_R2_001.fastp-trim.20220908.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-gonad-RNA-3_S17_L003_R2_001.fastp-trim.20220908.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences221
Sequences flagged as poor quality0
Sequence length68-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC146.334841628959276No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG62.7149321266968327Illumina Single End PCR Primer 1 (100% over 50bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT41.809954751131222Illumina Single End PCR Primer 1 (100% over 50bp)
TAAAAATGACTGGGTTCCACTGTCTTAAGGGGTCAGGAGGGGCCCAAAAG41.809954751131222No Hit
CTTCTCTATAAAGGAGCCCTGAAAAGTAGTTTTGTCCATCTGGTTGTTGT41.809954751131222No Hit
ATTTGGTCCCTGGTGTCTGAACAGACCCGTGAAAAGTATGCTAAGGAGAC31.3574660633484164No Hit
AGCCATCCCAGTCTTTAACTCCTCGACCTGACTTTTAACAAACAGTTACA31.3574660633484164No Hit
GTGGAGGTGGCGACTCCGTGATTGGAATCAGTGGACATCTGGGAAATCAA31.3574660633484164No Hit
GTCATCTTGGACATTCAACAATCCACCGTCGTGGCGTATGTTTACAAACT31.3574660633484164No Hit
TTTTGGCCATTTTTAATAGTTTTGAAAAGAAATGATATACTTTCCCTCTA20.904977375565611No Hit
GGCCGTTAAACAGTAACACCTTTTTGTCTCTGAATAAACCGCACTCACGA20.904977375565611No Hit
GACCCAGGTCGGAATCCTGTGACAAGTATCTTTTTTTCCCTAGCAACTAA20.904977375565611No Hit
CAAAGAGATACCTGAAGTACCTGACCAAGAAGTACTTGAAGAAGAACAAC20.904977375565611No Hit
CCCCCCCCCCCCCCCCCCCACCCCCCCCCCCCCCCCCCCCCCCCCCCCCC20.904977375565611No Hit
TGGATATCAATATTTTTTTTCTATTATCTCTTATTTTGATACTCCTGTTT20.904977375565611No Hit
GAAACTCGTGCTGTATTAACAATAAACATGGATAGTAACACGATATCAGT20.904977375565611No Hit
GGTACAGCAACAATCCGCACGCGCAAGTTCATGACGAATCGTCTGCTTTG20.904977375565611No Hit
GAGCGAAACTGTTTGTTACCTTCTTCTTTCTTCGATATCAGCTGTTTCGC20.904977375565611No Hit
CTACGTCTTGTAATGCTGGCCTGTCATTAAAGTTTACAAATTGTTTGAAT20.904977375565611No Hit
CTCTGATTCAGTCTGAGGTCTGCCTCCACCAGTTAAAAACTAGTTTAAAT20.904977375565611No Hit
ATTCAACCAGAGTGGTATTTCTTATTTGCTTACACGATCTTGCGGAGTAT20.904977375565611No Hit
CTGGATTAACAATGACCTTACTATAGCCATGGTTGGTGCACCGGTAGATC20.904977375565611No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.904977375565611No Hit
AAAGCCCTTCCCCTGGGGTGACGGGAACCATTCCCTTCTGCACAACAGCC20.904977375565611No Hit
CCTACATCAAAAAGATTGGTTACAATCCCAAAGCTGTTCCATTTGTACCA20.904977375565611No Hit
AATTTTTACATTTTGTTCAACACATTGACATATTGCAACACTGATCTCAT20.904977375565611No Hit
CGGCAACCAGACTCAGTCAAATTTGTGACCATTATTCAAAAACTGTGGAC20.904977375565611No Hit
GGAAAATATGGCGGCTATTATTAAGAGTGATGCGTAAAATGAATACTCGA20.904977375565611No Hit
GGATGATACAGAATTCTATATGGGAAAACGATGTGCATATGTATACAAAG10.4524886877828055No Hit
GATCAAACAAATTGAACATTGAAAAAAGCACATTGCAGCTTGAAATAAAA10.4524886877828055No Hit
CTGAATATGACATATTCAGTTTCATTTTTTGAGAAATATCTCAGAGAGAA10.4524886877828055No Hit
TTAGTTCCTTTGATACTTTCTGCTCCTGATATGGCATTCCCTCGAATAAA10.4524886877828055No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCACCCCCCCCG10.4524886877828055No Hit
GACGAGAACACACGTTGAGTGACTTGGTCTGATCGGTTGGTGACATTCGG10.4524886877828055No Hit
GTTGTTTATCATTTAATTAGCTGAATACTATGGATACTACATGTAGCTAA10.4524886877828055No Hit
TAGAGTAGTGCTAGAACAGTCTTCGGAAGCAACGGAATTTGATGCAAAAA10.4524886877828055No Hit
CTGCAGTGTCATCAGCAGAAATCTTGTGTTCCATCGAGAGAAAAAATACC10.4524886877828055No Hit
TTCATCTCTCACCACTGATTCAAGTGGGAAAGCTATTAGTTACTTGCTGA10.4524886877828055No Hit
TGACCTTCAGTAGCCGATGAAAATTGAATTGTTATATGCCAACCATTGCG10.4524886877828055No Hit
AAACCGTGGCCGTGGGTGTCATTAAGGAGGTGACAAAGGTTGAGGCTGAG10.4524886877828055No Hit
GGTGTATATAGAATACGTTTCTATGTTGTACTAATGCAGATGAGGGACAT10.4524886877828055No Hit
TTTCACTTATGACATCATCAGTACTAATTATCTAATTGATAAAATCATTT10.4524886877828055No Hit
AGATCAAGCCTTTCCTCAGAGTGTACAATTACAATCATTTGATGCCAACG10.4524886877828055No Hit
GTTGCTCGAACAGTTTCATCAACAAAACGTATGGCATTCATTTTTCAACT10.4524886877828055No Hit
CGGGACCCTTGTAACTTTCGTTTACTATACATATTGCCATTTGTTGATGA10.4524886877828055No Hit
TTTGAATGAACTTACAGACGGATGTAACTCGCTGCAAGAAAAAACCTTCA10.4524886877828055No Hit
CACCACTGAACGGTGCAATTAACTGTGAAAAACGTGCAACACAATTGGTT10.4524886877828055No Hit
NTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT10.4524886877828055Illumina Single End PCR Primer 1 (98% over 50bp)
ACTACCTGTTATTGACTTCTGCCAGATGTATTCTTCTGATTTGTTTACTT10.4524886877828055No Hit
CCCCCCCCCCCACCCTCCCCCCCCCCCCCCCCCCCCCACCCCTCGCCCCC10.4524886877828055No Hit
CCCCACACCCCCCCCCCCCCCCCCCCCCCCCCACCCCCCACCCCCCCACC10.4524886877828055No Hit
CCCCACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCACCCCCAC10.4524886877828055No Hit
ATTCACCGTTATCTTTTATAAAATATAATTTTTATGATCAATAGCATCAG10.4524886877828055No Hit
CCCCCCCCCCCCCCCCCCCCCCGCCCCCCCCCCCCCCCCCCCCCCCCCCC10.4524886877828055No Hit
CCCCCCCCCCCCCCCCCCCCCCCCACCCCCCCCCCCCCCCCCCCCCCCCC10.4524886877828055No Hit
CATTTTAATACCAGTTTAAACATAGGCCGGTCCATGTTTTTACGCCGTTT10.4524886877828055No Hit
ATCCTAACTTGTATTTTCATAATATGTAAATATAAAGGTATTAATAAAAA10.4524886877828055No Hit
AGGTTTAGAGGCAGAAAGAGTTTCAGTATTGAAAGACAAATTGCACTTGA10.4524886877828055No Hit
AATTTTTCCATTTTGTTCAACACATTGACATATTGCAACACTGATCTCAT10.4524886877828055No Hit
ATTGGCACTCTATTTTTACTAGTTGGGTTAGTTCGGACTTACATGAATCA10.4524886877828055No Hit
CCCCCCCCACCCCCCCCCCCCCACCCCCCCCCCCCCCCCCCCCCCCCCCC10.4524886877828055No Hit
TGCTGGATTCCGGATTTTACACACACTGGTGTATCTACTCGTTGTTCCGC10.4524886877828055No Hit
TCAAACTCGAATTTAAGTTTCATAACCTAGGTCCCAGTTATACATTAACT10.4524886877828055No Hit
AGCACCCCCCCCCCCCCCCCCCCCACCACCCCCCCCCCACCGCCCACCCC10.4524886877828055No Hit
TGGACGTATTGACAAACCCATACTGAAGGCTGGCAGAGCCTATCACAAGT10.4524886877828055No Hit
GCCGGAAAGACCTCAATAAAGATCAGAACAAAGAAAATGGATGTTCTGCC10.4524886877828055No Hit
CCCCCCCCCCCCCCCCCCCCCACGCCCCCCGCCCCCCCCCCCCCCCCCCC10.4524886877828055No Hit
TGGAATCACTGCTGACAATATTGAAGGCACATATAAGAAGGTTCATGCTG10.4524886877828055No Hit
GCATATTTTATGTAATTAAACAGATGGAGTATTAATACAGTTAACGTATT10.4524886877828055No Hit
CCGTTGCCATGACAATAGCAGCTGTCGCGTATGCCAGATATAATTAAAAC10.4524886877828055No Hit
CCACCCCCCCCCCCTCCCCCCCCCCCCCCCCGCCCACCCCCACCCACACC10.4524886877828055No Hit
CACATAGTCACCTATACCATATTTGAGAATCGCTTTTTTTTGTGCAAAAA10.4524886877828055No Hit
AATTTTTTCATTTTGTTCAACACATTGACATATTGCAACACTGATCTCAT10.4524886877828055No Hit
CCCCCCCCCCCCCGCCCCCGCCCCCCCGCCGCGCCCCCCCCGCCTCCCCC10.4524886877828055No Hit
CCCCCCACCCCCCCCCCCCCCCCCCCCACACCCCCCCCCCCCCCCCCCCC10.4524886877828055No Hit
AGACATTGTTATGTTTGTAATTGACATATTCATATTTTAAATTTCTCGTG10.4524886877828055No Hit
TTTCACCACTGATGGAAAACTTACTTGTTCACCTTCCTACATAATTTTTT10.4524886877828055No Hit
CCCCCCCCCCCCCCCCACCCCCCCCCCCCCCCCCCCCCCCTCACACACCC10.4524886877828055No Hit
CCCCCGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC10.4524886877828055No Hit
AAGATGCCTTTAACTATAAATTACTGGGACTGGCATGATTTTTTTTTTTT10.4524886877828055No Hit
TATTAATCGAAACGAAACTTTTGGGGCTGAAAGTTCGTTTTCGAAACTAA10.4524886877828055No Hit
CAAGAATATAATTCTGGCACAAATTAGGTGGCAAATTGCTTGTCAGTTGT10.4524886877828055No Hit
GTATTAACCTGGTATAAGTAGGACTAAGTGTAGTGAATAGAGTGTTGGCT10.4524886877828055No Hit
ATTGAAACTTATTTTGCTGCTACATTCCAAGCTGGAACTTTTTGTGATTC10.4524886877828055No Hit
CCATAGTGTATAAGTCATGGTCGACACCAAATTTATTGGTTTGGCAGAGG10.4524886877828055No Hit
AGCAACTTGTAATATTGATTTACTTTGATAATATAAGAACTGAACTGTGT10.4524886877828055No Hit
CCCCCCCCCCCCCCCGCCCCCCCCCCCCCCCCCCCCCACCCCCCCCCCCC10.4524886877828055No Hit
CCCCCCCACCCCACCCCCCCCCCCCCCCCCGACCCCCCCCACCCCCCCCC10.4524886877828055No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCACCCCCCCC10.4524886877828055No Hit
CCCCCCCCCCCCACCCCCCCCACCCCCCCCCCCCGCACCACACCCCCGCC10.4524886877828055No Hit
GCTATCCCTACAGGAATTAAAGTATTTAGTTGAATTGCTACAATCAGAGG10.4524886877828055No Hit
TGATCATCAACAAGAAGGTGGATAATGGTGTAAATGAAGTTGCCAGATTG10.4524886877828055No Hit
TTTAGTTCCTTTGATACTTTCTGCTCCTGATATGGCATTCCCTCGAATAA10.4524886877828055No Hit
TGAAAATGATCCTCTCTAGAGTTGAGTGTAAGTTTTCATCAAACACTGCA10.4524886877828055No Hit
CCACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC10.4524886877828055No Hit
CATTTTGCCTATGTTCAGGTGGTCTCCATCATTCAGCAAACTTGTGTTTC10.4524886877828055No Hit
TCGAATCCATTTTCCTTATGTTTAATGAATAAAGAGCCAAACCATGGCAT10.4524886877828055No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCACCCCCCCCCCCCCCCCCCCCCC10.4524886877828055No Hit
ACCCCCCCCCTCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC10.4524886877828055No Hit
ATTTGGCAGTCCTCAAAACACTAGAGATGAAGAAATGAGGAAAATCAAGA10.4524886877828055No Hit
GTCAAACATGAACAGAATATAGACTGTGGTGGTGGTTACGTCAAAGTCTT10.4524886877828055No Hit
TCCGCATATGCTTAGTACTGGTTCTACCCGGGAAGACTTCAAGTGTGATT10.4524886877828055No Hit
CGGATTCACCTCCCTCCTCATGGAACGTCTCAGCGTCGACTACGGTAAGA10.4524886877828055No Hit
GCCGCCATAAACCTCGCTAAGTACCGCAAGGTACAGAACGAACTTGAAGA10.4524886877828055No Hit
GAAACATTGAATTATGCCAAGAGAAAACATTACAAGTTTAATGTATTTGC10.4524886877828055No Hit
CCCACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCACC10.4524886877828055No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCACACACCCC10.4524886877828055No Hit
AACACTTGGCTCATTTGATCAACATGACATAATTAACACACTTTCAATCT10.4524886877828055No Hit
TGAAAATCAACTAACCCAAAAAGTTCAAATTCTTGGTTCAGATTTGTTTC10.4524886877828055No Hit
TTTATGGACATGCTCTGAGCTGAAAGACGGGTTGTCCTCACCACGTAAAT10.4524886877828055No Hit
GCCGACTGTGCCAACCACACATGTCACCATGGGTCTTACCATTGTGAAAT10.4524886877828055No Hit
GACCTCTCAGCAAGACAGTCAAATTCAATGTTGTTAAAGTGTCTAAGGGA10.4524886877828055No Hit
GCTCCACATATTTTTTCTTTAAAAGAATTCAGTCGAAAATAATAAATAAA10.4524886877828055No Hit
GTTTTGACCCTTTGAGAAGGTCTCGATTAACTTTTTCATTGCGGTTTTTT10.4524886877828055No Hit
CTGAATATGACATATTCAGTTTCATTTTTTGTTTTTTTTCTCAGAGAGAA10.4524886877828055No Hit
TTCAAGCAGAGGTAGACTTAGAAGAAAGTTCAGAAACATCGAAAAAATCA10.4524886877828055No Hit
TTTGTAGCAGTTGGATGATAGAGGATTTGATTACGTCGATTATAGTTTTA10.4524886877828055No Hit
GTGGCACATGTGTCATGCATCAGTGTAAAAGTGATCACTGAAACTTCAGC10.4524886877828055No Hit
CAAAATTCCAGACTGGTTCCTCAACAGACAGAAGGATGTCAAGGATGGTA10.4524886877828055No Hit
CTTTAAGTTAAAAGTTTAGTTTATACTAATTTATTTTAGCTTGATTGAAA10.4524886877828055No Hit
GGCAAGTGGAATGCATGGTTAACGTGTAAAAACCTAATTTCTAATTTTTT10.4524886877828055No Hit
CAGATCAACCAAAATATCGTCTCCCATAAACGTGTCACTATTGAGACGGA10.4524886877828055No Hit
CACACAGTGTCGGCGATGTGGGCGCCGGGCGTATCATATTCAGAAGAAAA10.4524886877828055No Hit
ACCCCCCCCCGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCAC10.4524886877828055No Hit
ACTGCATCCCTCCCTCACAATGATTATGTCCAGGTTTGTTGGGTAAGTAC10.4524886877828055No Hit
CGTTTGTGATCGTTTATGTGATGCACACTGTGCCAATTGTGATCGTTCTT10.4524886877828055No Hit
GTGCTGGTACTGTGCGACGTCAAGCTGTGGATGTGTCGCCTTTGAGACGT10.4524886877828055No Hit
ATTGATTCCATGGACCAATCAAAGTCCTCAACTTTATGAGTGAAAATGGA10.4524886877828055No Hit
CCGGGCGTATCATATTCAGAAGAAAAGGTGTGCCTCATGTAGCTACCCAA10.4524886877828055No Hit
GTTGGATTACCAGTATCTGTGGCTTAATTCTGTATCTTTAAAATAAATAT10.4524886877828055No Hit
CACCCCCCCCCACCCCCCCCCCCACCCCCCCCCACCCCCCACCCCCCCAC10.4524886877828055No Hit
GTTGGTACGGGTCATATAATAGGATAAATATTCATCGTATAGTTGGTTAC10.4524886877828055No Hit
GAAATATTTAAAAAAAAATAGATTAAAAAAAGCATCCAGGTGATCATGAT10.4524886877828055No Hit
CGAAACTGGTGAATTTGTGGACATGTATATCCCAAGAAAATGCTCTGCAA10.4524886877828055No Hit
GTTGATATACAAGAGAGGGTACGGTAAGGTCCACAGCAGAAGGGTCCCAC10.4524886877828055No Hit
GGGCAGTCACCTCTGTTGAGCAGTTACTTAGAAATATTAAGCAGCCACAT10.4524886877828055No Hit
CCCCCCCCCCCACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCACCCCCCC10.4524886877828055No Hit
GCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCACCCCCCCCCCCCCCCC10.4524886877828055No Hit
AGTTTAGGGTGAAAATTTTGTTATTATTATATTCAGATAAATAAAGTTTG10.4524886877828055No Hit
CTCCGATCTTATGGACAAATCCGCCGAGTCCCATCCACGTTGCTTCGAAA10.4524886877828055No Hit
TTTGGTTTCAATAGTCTTGGTTTATTTGATAGTTGTACAGAATGTATTTA10.4524886877828055No Hit
AAAATCCGGCGTTTGTTTAACCTGGGCAAGGAAGACGATGTCAGACAATA10.4524886877828055No Hit
NTGAAACAGCAGACGCTCTATCTTTCGTCAGCAATAATATGCTGAGCGAA10.4524886877828055No Hit
TGTTAGTCTCGAGTGTAGGTGTGACAATGTTCTGGTACTCTCGAGTGTAG10.4524886877828055No Hit
TTAGGCTTCGTAGTATGGGGTCATCACATATTTACGGTTGGCATAAATAT10.4524886877828055No Hit
GAAACTGGGCCCCAAAGTTTTATTTTTGTAATTAAGTTAAAAATAAATTG10.4524886877828055No Hit
GATTTTTCCATTTTATGACGTTGTTGCTTCTTGCTGCTAATTAGTGAAAC10.4524886877828055No Hit
CCTGTGTTGGCGGGGGCTATTACAATATTATTAACAGATCGTAATTTTAATACCTCATTTTTTGATCC10.4524886877828055No Hit
GCTGGGCAAGGGCGAGTTTGTCTCGGGCTCGATCACAGTGGAGTTCTTTG10.4524886877828055No Hit
AGGCTCTCTACGATATCTGCTTCAGAACACTCAAACTGACCACCCCAACA10.4524886877828055No Hit
ATGACACACAGGATATAATTGTAGATAAACTTGGACTAAATACAGAACAA10.4524886877828055No Hit
TTCAAGCAGAGGTAGACTTAGAAGAAAGTTCAGAACCATCGAAAAAATCA10.4524886877828055No Hit
CAAAATTGTAACTTCAAATGATTTATTTTCTATTTATTTGATAATTTTTT10.4524886877828055No Hit
TTTTACACCAAATCCGAAATTGCTCAAAAATAATACATACATGTAGTATT10.4524886877828055No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCACCCCCCCCCCCC10.4524886877828055No Hit
TTTTTTATTTATGTTTTTACCTCATTTGCATTTCAAGTTCAAATTTATAT10.4524886877828055No Hit
CCTTGAGCCAGACACCTTATCCGCATATGCTTAGTACTGGTTCTTCCCAG10.4524886877828055No Hit
TTTTGGACAAGTACACACAAATTTCTCGCATTCTCTCTCCGATTGTGACC10.4524886877828055No Hit
ATTCTGTAATGTTTATGAAACACTAAATGAAGTTTGTGAACTTTTTTTTT10.4524886877828055No Hit
AAAAAATACATAAGACTCTTACTTTCACAAAGTGTACATCTGTGTAAGAA10.4524886877828055No Hit
TTTTACTTGGAATGTTGTTATTAAGGGTGTTGGGGTTCTTTGTGTTGGGT10.4524886877828055No Hit
CGCAGATTTGTCATGGTAAAAAGACGTTATTATGAGACGTAACGTCTTGT10.4524886877828055No Hit
CCCCCCCAAGACCCCCCACCCCCCGACCACCACCTCACACCCCCACCCCC10.4524886877828055No Hit
CAACAATTTAACAGCGGCATAACCAGTAAGTTCATCCATTTTAATTCAAG10.4524886877828055No Hit
GTGAGATATACCCATTGTGAAATATACACAGTGTGAGATATACTAGTTCA10.4524886877828055No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCACCCCCCCCCCCACCCCCCCCC10.4524886877828055No Hit
TTTATACGTAAATACTTGTATGATTTACAGTTGCTGAAAATTGTTTTGAT10.4524886877828055No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)