FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-gonad-RNA-3_S17_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-gonad-RNA-3_S17_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1104
Sequences flagged as poor quality0
Sequence length68-151
%GC63

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC252.2644927536231885No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC151.358695652173913TruSeq Adapter, Index 6 (100% over 50bp)
CCCCCCGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC60.5434782608695652No Hit
ACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC50.4528985507246377No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGT50.4528985507246377TruSeq Adapter, Index 6 (100% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC50.4528985507246377TruSeq Adapter, Index 6 (100% over 50bp)
GGACAGTGTTAATTGACTAAAAACTGGCGATATTAAATTTATGAAACAAT40.36231884057971014No Hit
ATTATCTGCAATCACAAACCCATACACACAGGGATAATTTATGCCAAATA40.36231884057971014No Hit
ATTTCACAACAAATGGGTATAATAAACATGGACATTAACAGTATCACAAA40.36231884057971014No Hit
TCTTTATTTCCACCCAGTATGTACAGTTATGCGACTAACACACATGCACC30.2717391304347826No Hit
CACACATACATTATGTTCCAACTCTTCTAATGTATACACAATAACATGAT30.2717391304347826No Hit
CCACTGCAGACTTGCGGTCATGCCTTACAGAGACGTCACCAACCTGTCCT30.2717391304347826No Hit
AAAAAAGTTAAAATCATAAACACACCTACAAAGTATCAAAAAAAATACTG20.18115942028985507No Hit
ATTATATTGATTGGGATCAGATGCCTTTAATGTAACTCTGAAGGAAAAAA20.18115942028985507No Hit
CAACAAATCACAAGGACGACAGCAGACAACGCAATGTCTATTCATCAGCA20.18115942028985507No Hit
AGCATCTATAAAAACAACTCCTGATTAAACACTCAATGCTCCCCATCTTC20.18115942028985507No Hit
TTTTTACCTAAATTGTCTTTATATTATTCATGAAATGTACACTCAGTTTT20.18115942028985507No Hit
TCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC20.18115942028985507No Hit
CGGTCTCGTACACATAGTTCTTAAGTATTACAAAAGCTTCATCAACAGTA20.18115942028985507No Hit
TTTTATAGTCCATGCATTTAATATAAATATTCACATTACAAATAACAGTA20.18115942028985507No Hit
GCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC20.18115942028985507No Hit
GTCGTCACTTCCAGTTGAGTTTAAGCTCAAATTCATCAGTTTATGTTTAG20.18115942028985507No Hit
CTCAACACTCCAGCCTTTGTACCATGACATATTACTAGATTCCTCTACCA20.18115942028985507No Hit
CAAGTTCTTAGACTCGCACCGGATCATCAGAAATCAAAAGATACGTACAA20.18115942028985507No Hit
CTCAAGCTAAGATTTTCCTCATCTCAAAAATCTCAATTTCTCTAAATAAC20.18115942028985507No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCACCCCCCCCCCCCCCCCCCCCC20.18115942028985507No Hit
CTTTGTAAGGCGGCACTACCAACAACAACCATTGGGTTCTTTGCCTTGGC20.18115942028985507No Hit
TTCCAGAAAGCAATTCTGTGCGTCCCTGTTAATGTTAATTTCCTTAATAA20.18115942028985507No Hit
CATAGGTATTGAATGTTTACTAAACTGTATTGTTGCTTTTTGTTTTTTTT20.18115942028985507No Hit
GGGTCCTCCATGGCCAATTGGTCTTCCTTTCAGTCTCAAGATCATTAAAC20.18115942028985507No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)