FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-gonad-RNA-3_S17_L003_R1_001.fastp-trim.20220908.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-gonad-RNA-3_S17_L003_R1_001.fastp-trim.20220908.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences221
Sequences flagged as poor quality0
Sequence length68-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC135.88235294117647No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC62.7149321266968327TruSeq Adapter, Index 6 (100% over 50bp)
CCCCCCGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC52.262443438914027No Hit
ATTATCTGCAATCACAAACCCATACACACAGGGATAATTTATGCCAAATA41.809954751131222No Hit
GGACAGTGTTAATTGACTAAAAACTGGCGATATTAAATTTATGAAACAAT41.809954751131222No Hit
ATTTCACAACAAATGGGTATAATAAACATGGACATTAACAGTATCACAAA41.809954751131222No Hit
CACACATACATTATGTTCCAACTCTTCTAATGTATACACAATAACATGAT31.3574660633484164No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC31.3574660633484164TruSeq Adapter, Index 6 (100% over 50bp)
CCACTGCAGACTTGCGGTCATGCCTTACAGAGACGTCACCAACCTGTCCT31.3574660633484164No Hit
TCTTTATTTCCACCCAGTATGTACAGTTATGCGACTAACACACATGCACC31.3574660633484164No Hit
TTTTATAGTCCATGCATTTAATATAAATATTCACATTACAAATAACAGTA20.904977375565611No Hit
CAACAAATCACAAGGACGACAGCAGACAACGCAATGTCTATTCATCAGCA20.904977375565611No Hit
AGCATCTATAAAAACAACTCCTGATTAAACACTCAATGCTCCCCATCTTC20.904977375565611No Hit
CAAGTTCTTAGACTCGCACCGGATCATCAGAAATCAAAAGATACGTACAA20.904977375565611No Hit
ACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC20.904977375565611No Hit
TTTTTACCTAAATTGTCTTTATATTATTCATGAAATGTACACTCAGTTTT20.904977375565611No Hit
CGGTCTCGTACACATAGTTCTTAAGTATTACAAAAGCTTCATCAACAGTA20.904977375565611No Hit
GGGTCCTCCATGGCCAATTGGTCTTCCTTTCAGTCTCAAGATCATTAAAC20.904977375565611No Hit
GTCGTCACTTCCAGTTGAGTTTAAGCTCAAATTCATCAGTTTATGTTTAG20.904977375565611No Hit
CTCAACACTCCAGCCTTTGTACCATGACATATTACTAGATTCCTCTACCA20.904977375565611No Hit
CATAGGTATTGAATGTTTACTAAACTGTATTGTTGCTTTTTGTTTTTTTT20.904977375565611No Hit
TTCCAGAAAGCAATTCTGTGCGTCCCTGTTAATGTTAATTTCCTTAATAA20.904977375565611No Hit
AAAAAAGTTAAAATCATAAACACACCTACAAAGTATCAAAAAAAATACTG20.904977375565611No Hit
CTTTGTAAGGCGGCACTACCAACAACAACCATTGGGTTCTTTGCCTTGGC20.904977375565611No Hit
ATTATATTGATTGGGATCAGATGCCTTTAATGTAACTCTGAAGGAAAAAA20.904977375565611No Hit
GGATCAAAAAATGAGGTATTAAAATTACGATCTGTTAATAATATTGTAATAGCCCCCGCCAACACAGG10.4524886877828055No Hit
CTCTGATTGTAGCAATTCAACTAAATACTTTAATTCCTGTAGGGATAGCA10.4524886877828055No Hit
GTCTGTGTCCCGGAAATTAAATTTGAGAATGTAAGCAAATATTTGCTTTT10.4524886877828055No Hit
CCCCCCCCCCGCCACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC10.4524886877828055No Hit
GCCCGTTGTTACGCAAAGTCCTGTATCACATGTATACATTGCACAAAACC10.4524886877828055No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.4524886877828055No Hit
CAATGAATGAATACTGAATTAAAATACTAGTTAGAATGCTAACTTTTCAA10.4524886877828055No Hit
ACCGCCCCCCCCCCCCCCACCCCCCACCCCCCCCCCCCCCCCCCCACCCC10.4524886877828055No Hit
ATTACTCCCATGTTAGCAAAAAAACACAAAGCACGTGCGGGCTGCGGAAC10.4524886877828055No Hit
CCCCCCCCGACCCCCCCCCGCCCCCCCCCCCCCCCCCCCCAACCCCCCCA10.4524886877828055No Hit
TACAAAATATATTTAAATTAATCATGTTTACATTCGGTACAATTTACAAG10.4524886877828055No Hit
CATTTTCACATTATCTTTGCCATATAATTCCTTCACGTTAGCATATGATG10.4524886877828055No Hit
CAAAAAATAACTTTCATAAATCTAAAGATATCTTATTTATAAAAAGAAAA10.4524886877828055No Hit
CTTAAATGTCGTCTTTACCAGCGTCAACGCATACAACTCCTGACATCTGT10.4524886877828055No Hit
CCGCCAGATGAAGAGAAAAAATCCCATAATCAACAGAGCCGCCTCTATGG10.4524886877828055No Hit
CTCGGGCGCTCTCTTTACATCTGGCGCATGACAGTTACAATGGCCGTGTC10.4524886877828055No Hit
GAAAGAGGCACTCACTCTTCTATATACCCTATACACATAGGGCGCGATTA10.4524886877828055No Hit
AAGTTGATTATTTCATATTAATTCATTTTATCTTGATTGTGATGACAGCT10.4524886877828055No Hit
TAACCATCCTTCATGGACCTCATACGTCCGGTGCCTGTGGTCTTCCTGCG10.4524886877828055No Hit
CACTAAACTGCAATTAGATATATAATGATAATAGTGTTTGTCTATGACAA10.4524886877828055No Hit
TTTTTCAAACTAAGAGCCCCATCAATAAACAAAGATAAACAAAAAAATCC10.4524886877828055No Hit
TCTGTTGTGAATTTTATTAGATGGCACTTTTTTACCAAAACAAGTATTCT10.4524886877828055No Hit
AAAAATATTCAATTTAAAAAAAAATAAATTTAAATCAATTTTTTTTTACG10.4524886877828055No Hit
TTTTTTTTAATGGATTTTATATATATTTTATTTGAAATAATGGACAACAG10.4524886877828055No Hit
AGGTTTAGAGGCAGAAAGAGTTTCAGTATTGAAAGACAAATTGCACTTGA10.4524886877828055No Hit
GGACTTTATACAAAGACTGAATTATGGAAGAAACGAAGAAAAGAATGACT10.4524886877828055No Hit
GGACCTACTAAAGTGTGTTGAAAAGTAAACGTAGAGACCTACTAAAGTGT10.4524886877828055No Hit
GTTCCTTGTAACAGGAGTTTAATCCAAATCAAATGAAGCAATGGAAGTAT10.4524886877828055No Hit
CCCACCCCCCCCCCCCCCCCCCCCCCCCCCCACCCCCCCCCCCCCCCCCC10.4524886877828055No Hit
GGTTATCACTATAAAAAGTTTTACCTGTCTGTTTATCAGTGCCACTGTAA10.4524886877828055No Hit
TTTTTGTCCAATGTCTGAATGTGCTGTTGCGTACACCAAGACACAAGGAA10.4524886877828055No Hit
ACATGTTAAACATAAGTGCCGAAGCAGACGTTTTCCACTTTGATTCCAAA10.4524886877828055No Hit
CAAATTTCTACAATTATAAACAAAGAAAAATTGCCTCTATTGCTTATTTA10.4524886877828055No Hit
CTTGATTTTGCTTTTTCATAGAGACCATGTCTTGCCAATCTGTATTTAGG10.4524886877828055No Hit
ATGTCCACGCCCCGGTGTCTTTCATACACACTCGCTTGATAAGTAGTTCG10.4524886877828055No Hit
CCAAAAACATTTTTTTTAACAAGTTAACAAAGGTAACAATTAATGCCATT10.4524886877828055No Hit
CTCGAGAGTACCAGAACATTGTCACACCTACACTCGAGAGTACCAGCACA10.4524886877828055No Hit
CTTCTTTGTACCTGGTCCGCAAATATCAGGTCCGAACATTATGTTGTATG10.4524886877828055No Hit
GAGCATTTCAGAATTTCCTGAATCCCTTCTTTGAGGTTGTTCCCTTAGAC10.4524886877828055No Hit
AATTAACCAATTTCATAATAGCCTGGTGTTTAAACAAAAGATAATGATTT10.4524886877828055No Hit
GCCCCCCCCCCCCCCCCCCCCACCCCCCCCCCCCCCCCCTCCCCCCCCCC10.4524886877828055No Hit
AAATTCTTCCTCAAGTAAGCTTATAATTCACTTTTAATAAAAAAAGAAAA10.4524886877828055No Hit
CCCCCCGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCACCGCCC10.4524886877828055No Hit
CCGCCCCCCCCCCCCCCCCCCCCCCCGCTCCCCCCCCCCCCCACCTCCCC10.4524886877828055No Hit
TTCGCGGCAACATCGATGTAAAGCATGTATGAAAAAGAGACCACATCTAA10.4524886877828055No Hit
GCAACTTGTAAATGCTTCATATGAGATTTACGGTAAATCCAACGCTTTTA10.4524886877828055No Hit
NTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.4524886877828055No Hit
AGAACAATATGTAAAAATAAAGTCATAATTTCAGACAAAAATGAAAGTAG10.4524886877828055No Hit
GACTTGTCGTGCTGCACAACCATCTGCATTTATCCAAAAGTATTTCTTAT10.4524886877828055No Hit
TCTTACACCAAATTCGAAATTGCTCAAAAATAATACATACATGTAGTATT10.4524886877828055No Hit
GGACAATTTGAACCCATCCGTCATTCCTTAATGTATTTTATTTTTCAATT10.4524886877828055No Hit
CTTCCATCAAATGCAGATCGGAAAAAATCAGCCAAAATTTCTTTTCTACA10.4524886877828055No Hit
CAAGCATTACCGTTAAACATAAGCTGAATCTATCCTCTCAACCTGACAAC10.4524886877828055No Hit
CCGCGAAGTATGTTTTCTTTTCAAACACTGGCGCGTATCTGTCCACGTCA10.4524886877828055No Hit
GNATATACTAGGGTCTTCTGAAGGATCCATACATAAAAACTCGCCATAAA10.4524886877828055No Hit
TTATCTACAAGTATAACCTGTGTGTCATTTGTTTCTATAATCTGTTCAAT10.4524886877828055No Hit
TATACTTAAAACAAAAACATAAACAAAACAAAAAACAACAGTATACACGG10.4524886877828055No Hit
CCAAAATTAAGTAAAATTACAAAACACTGTTAATCAAGTGTATTCATAGT10.4524886877828055No Hit
AACAAAACAACCTATAAAAAGAAGGCCAATAACACCAGCTAACATGGGTA10.4524886877828055No Hit
GGTGCCTGTGGTCTTCCTGCGCTTAGCCTTAACACTCCAGTTAAAGTGTC10.4524886877828055No Hit
NTCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC10.4524886877828055TruSeq Adapter, Index 6 (98% over 50bp)
TTCTCACCAACAAATATTTTAATATAATTTTGTTAATTGTTCAATTGTTG10.4524886877828055No Hit
CTCTACCAAGCATTTTATCATTTTCTCCGAGTTTACTAAATTCAATAATA10.4524886877828055No Hit
CCCGCCCCCCCCCCCCCCACCCCCCCCCCCCCACCCCCCCCCCCCCCCCC10.4524886877828055No Hit
ATTCTAAAACCAAAAATATTTTCAATAACGAGGAATAGGACAATTATACA10.4524886877828055No Hit
GGAGACTGTTGACCGCTCATGCCAGAGGTAGTGGGTTCGATCCCTATCTT10.4524886877828055No Hit
CAAGGGTGGTATGGGTGGTCAAGATTGAGTTGTATGGCTCAACGACGGCG10.4524886877828055No Hit
CACCCCCCCCCCCCCACCCCCCCCCTCCCGCCCCCCAACCCCCCCCCCCC10.4524886877828055No Hit
GCTGTACGCTTTCGTTCTTACTCCTTTTATATTATATTTTTCCACCAATG10.4524886877828055No Hit
TTTTTTTATTATAAACTATATTTTATTCTTCAACTCAAGTACAAACATAT10.4524886877828055No Hit
CTCAAGCTAAGATTTTCCTCATCTCAAAAATCTCAATTTCTCTAAATAAC10.4524886877828055No Hit
CAACAGGTCCAGGCTGACAGACTTCAATTTCACTTGGCGGTGCAAAACGA10.4524886877828055No Hit
CTCCTGTTTCACAAACATTTTTCTTAAATTTTACTTAAATAATACTTAAA10.4524886877828055No Hit
GCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC10.4524886877828055No Hit
TGACTTCCAAAGACTGCCTAGTTCCAAATAATTTTATATTTATTCCTAAC10.4524886877828055No Hit
AGGAATATGTGTTGAACCAAAAAATGTTTTACTCAAAACACAAGCAAAGC10.4524886877828055No Hit
TTTTTTTAATGGATTTTATATATATTTTATTTGAAATAATGGACAACAGA10.4524886877828055No Hit
ACAAGTTACAACACCTGAAAATAGTTGTCTTTTCCTTCCCAACGACAGCT10.4524886877828055No Hit
TTTACTTAAAACAAAAACATAAACAAAACAAAAAACAACAGTATACACGG10.4524886877828055No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCACCCCCCCCCCCCCCCCCCCCC10.4524886877828055No Hit
CAACAAATAACTTTCATAAATCTAAAGATATCTTATTTATAAAAAGAAAA10.4524886877828055No Hit
CAGATCCACCAAAATATCGTCTCCCATAAACGTGTCACTATTGAGACGGA10.4524886877828055No Hit
TTTTTTTAATATTTATCTTATAATATACATATAATAATGGCAACAACCAA10.4524886877828055No Hit
TTTTTCCCTAAATTGTCTTTATATTATTCATGAAATGTACACTCAGTTTT10.4524886877828055No Hit
GCATTTTCTCACAGTGAGTTACACCTATTTGTAATATTTACCCTTTTAAA10.4524886877828055No Hit
TTGATGATCCCTTTGCTGCATTGATCAGTTCATCAGCAAGACACTCAGCG10.4524886877828055No Hit
TAGCGATAGTGCCTAACATCACTGGATCATAGAGGGTCTCTTCCTCTGAC10.4524886877828055No Hit
ACGAGCTGTAAGAGGAGCGAATCCAGGCATGAAGAAGTGGAGACGGGGGA10.4524886877828055No Hit
CTTGCTTTTGCTTTTTCATAGAGACCATGTCTTGCCAATCTGTATTTAGG10.4524886877828055No Hit
AGACCGGCTAAAGATTAATGATTCACTAGGAATTGACAAATATCAAAATA10.4524886877828055No Hit
TCCCCCGCCCCCCCCCCCCCCCCCCCCCCCCGCCCCCACCCCCCCCCCCC10.4524886877828055No Hit
TTTATTTTTCTCTTCATAAGGCTTTATTACATGTTTTAGCACATAAATTA10.4524886877828055No Hit
GCTCCACATATTTTTTCTTTAAAAGAATTCAGTCGAAAATAATAAATAAA10.4524886877828055No Hit
GGCCCCGCCCCCCCCCCCCCACCCCCCCCCCCCCAACCCCCACCCCCCCC10.4524886877828055No Hit
GCCGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC10.4524886877828055No Hit
TCTTAATTCAAATATCAACAATAATAATAGAGAGTCTAAATACATATGAT10.4524886877828055No Hit
CATACAGCTCTTTGGCTCCAGGGAACCACTTAATATTGAGACAGCAGTGT10.4524886877828055No Hit
CCAATGTTTATTCCTTGTCCTTTCCCTTAATAACGTTGGTTCCACGGAGA10.4524886877828055No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCAGCCCCCCCCCCC10.4524886877828055No Hit
ACGAATACAGGGTAAGAATGCACATGCGTTGTTATATATCTGTATAAAGA10.4524886877828055No Hit
ACCCGCGCCCCGACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC10.4524886877828055No Hit
NCGGCATCTCGTAGTGCCTGGGATTCGATGACGGTTCTATTTGGATACAT10.4524886877828055No Hit
CACAATTCTATTCTCATATAAACAAATTTACAGATGAAGTGAAAAAAATT10.4524886877828055No Hit
ATGTTTCAGACTAGACATGCAATTTCATGTTTTTTACATTAAAGCATGGC10.4524886877828055No Hit
GCCCCCGCCCCCCCCCCCCCCCCCCCCTCCCCCCCCCCCACCCCCCCCCC10.4524886877828055No Hit
CTCAAATCTTGTCTTAACTTCTCTCTTTGCCTTACGTTTCTTGGCAGGGT10.4524886877828055No Hit
CAACTTGAGCCCAAATTTATTTCGTCGCTTATAGACCATCTAACAAGATA10.4524886877828055No Hit
CCCCCCGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCACCCCCCCCCCCC10.4524886877828055No Hit
CGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC10.4524886877828055No Hit
GCAGCCCCCAATAATGTCGGAGACCTCTGTGGGCTCGGATTTTCTTCAAT10.4524886877828055No Hit
ACTGGACCGACGTCTCTTAAGAGCAAGACGGTGTCGTTTCCTCTGGATAA10.4524886877828055No Hit
CAAGCACCAGCAAAAAAGCAGTGATAGCAACTGACCATACAAATAAGCTA10.4524886877828055No Hit
CTCCACAATGGGTTAATCTGTGTGTCTCTGAGTCCACAGTCACCACCATC10.4524886877828055No Hit
TGAAACTATTAATCAAATGAATAGCAAGTGTGCTATCATTCACAGAGTAA10.4524886877828055No Hit
TTTCATGGGGGGCCTTGACTCCACAGAAGGGTCTTGGATAAAATTTTGTT10.4524886877828055No Hit
CCGGCCCCCCCGCCCCCCCCCCACACCCCCCCCCCCCCCCTCCCACGCCC10.4524886877828055No Hit
AGAAAAAGCAGTAGTCTAAGAAAAATAATTATTTTTAACCACAACTTTCT10.4524886877828055No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCACCCCCGCCCACCCCCGC10.4524886877828055No Hit
CCCCCCCCACCCCCCCCCCCCCCCCCCCCCACCCCCCCCCCCCCCCCCCC10.4524886877828055No Hit
GCAACATATACGATACATCGTTAATTTGCTGAGAGCATAGAAAATGGAAA10.4524886877828055No Hit
GTTTTCTTGACGCAGCATTTACACTTGTGTCTGGAGCAATGACAAAGTTT10.4524886877828055No Hit
CAAATGTTTTGTATGTGCCGGTCATTCTACCAGTGGTATCATCAACATCA10.4524886877828055No Hit
CAACCATATTTAGGCAGTTTATTTATTTTATGTTTACAGACAATTACACT10.4524886877828055No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCACCCCCCCCCCCCCCCCCCCC10.4524886877828055No Hit
CTCCGTCCTCTGCATTCAACTGTTCTGTATATAACTGAACTGAAATATTA10.4524886877828055No Hit
ACCCCCCCCCCACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCACCCC10.4524886877828055No Hit
TAAAAACAGCACCAAGTAATTACATACATGATATACTTATACGAGTTGCC10.4524886877828055No Hit
GCTTTCTTGACGCAGCATTTACACTTGTGTCTGGAGCAATGACAAAGTTT10.4524886877828055No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCTCCCCCCCCCACCCCCCCCCCCCC10.4524886877828055No Hit
CATGAATAAGTTTTAACAGAAAATATTTAGAGAAAAAAGTATCAATATCA10.4524886877828055No Hit
GAGAGAGGGTAGATCCAACCTGACGCAATTAGTTCATGGAATTTTCGCGA10.4524886877828055No Hit
GTTTGATGGTTATGATTTAGCAAACTATGTTAAAATGTAAAATTCAGTTT10.4524886877828055No Hit
GTAAAACTGTATGTAGATAGTACTGGCCAAGATTACTATCTGAAACCTGT10.4524886877828055No Hit
AGCAAATACTGCCCCTATACTTAAGACATAATGAAAATGTGCTACGACAT10.4524886877828055No Hit
AGTTAATACATCTTATTTGTTATCAATCTTTAGATGTTTTATACTATATT10.4524886877828055No Hit
CCTGGGGACATGAGACGGAACTGTTAAGATAATTGCGAACACCATAACAT10.4524886877828055No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGAATATCTCGTATGCC10.4524886877828055TruSeq Adapter, Index 6 (98% over 50bp)
TCGACTTTTACTGATTTCAAGTTCTTAAGGAAGTTTCATGTACAATCTAT10.4524886877828055No Hit
CACAGCCCTCGAGCCATTGTTCATGTTCCAGGCCCAGTATCACCTCCCGA10.4524886877828055No Hit
CCCGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC10.4524886877828055No Hit
AACAGACACACACCTCCAAAACAAATGATTGACATATTCAGTAATTAAAA10.4524886877828055No Hit
GCAAATGAGCACTTTTTTTTTCATGTATGTACAAATTTCTAGAAGTTTCA10.4524886877828055No Hit
CAGGTGTATAAAACATAGACGGATTTATACAATGAATAAAATCACACAAA10.4524886877828055No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)