FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-gonad-RNA-2_S9_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-gonad-RNA-2_S9_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2151
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC1838.507670850767084No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG80.3719200371920037Illumina Single End PCR Primer 1 (100% over 50bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT50.23245002324500233Illumina Single End PCR Primer 1 (100% over 50bp)
CCCAAAATATGATTTTTTTTATAAATTAAAGGGACATAACTCTACGACAA40.18596001859600186No Hit
TTCGAATAAAATACCTTTCATTGCAATGAATCGACAGAGAAGAATGCAAA30.1394700139470014No Hit
CACATAGAGCTATACTCTAAATACTGAACACCATGCAAGTGAGACAGAAC30.1394700139470014No Hit
CCGAGATTGTGTTTGCCCGTACATCACCGCAACAGAAGTTGATCATTGTA30.1394700139470014No Hit
CCCTGATATTAGTATGTCGACAAGTGTTTGATTTGGTCCATTGTAGAAAA30.1394700139470014No Hit
TCATCACAAGTAGGTACAGATTCAAGCTTGTCCACTCAGGGTATATGACA30.1394700139470014No Hit
CAACAGTCTCCGTGATGATTGATATACGACACTGCGTCTCATTATCATTT30.1394700139470014No Hit
GCGAGAGGTCGTTGGTGGCGAGCTGGTATCGACCATGACAGTTGGAGGTG30.1394700139470014No Hit
GGGGCTACCATGTATGTTTTTGTTTGTACATTTGATTCGAGTGTATTTTG30.1394700139470014No Hit
GGCTGGTGTTTTTTTTTACTCTCGGTTTCCTGTATAACATTACTTTTAGC30.1394700139470014No Hit
CCTAACCCCGTCAGCATTTCTCTCATTAACTTTGACCTTTCATTTTCCAT30.1394700139470014No Hit
CCAATACGTATTAGAACCAGCCTTCCGTGATGCCTTTGCCAATGTGAACA30.1394700139470014No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)