FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-gonad-RNA-1_S1_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-gonad-RNA-1_S1_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1137
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1109.674582233948989Illumina Single End PCR Primer 1 (100% over 50bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT504.397537379067722Illumina Single End PCR Primer 1 (100% over 50bp)
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC151.3192612137203166No Hit
GCCGTTCCTGGGCGGTTGAATATGCTGACTTTAAAGAGAAATCATGTTGG110.9674582233948988No Hit
CCCTCCCGCACATGCAATGTAAATAATGTAGGTTTTCATTCATTTAGTAT100.8795074758135445No Hit
TTTGCATTTGATTTCTCATGGCTGCACACTTGATTTCTCATGGATGCACA90.79155672823219No Hit
TATATCAGAAGAAAAGGCACAGCCAGTTGCAAGTTCTAAAAGGGTATCTC70.6156552330694811No Hit
GTTTCATGATCCAAACAAAGCTTCCTATATACATACATGTCACCTGACCA70.6156552330694811No Hit
TTTACGAATGGCTGTTTTCAAGACTGGTGGTAATAAAAAAAAACACAAAC70.6156552330694811No Hit
CTTTTTAATGTGTTCTTGGATTCTGATTGCTAGAATTTTGTTAAGGATTT60.5277044854881267No Hit
GAAATGGTGTTTATCTGTCTGCAACTAGTCTATCACAGAGCCTGATACCT60.5277044854881267No Hit
CACAGGTGAGAAATATATCTCAAGACTTAAACTTTCTGAATTCTATTCTT60.5277044854881267No Hit
CATGCTTCCATCCAAATCAACTTTGTTGATGTTGATGATACCACTGGTAG60.5277044854881267No Hit
TTTTAAGCTAAAACAGAACGGTGTTTGAAATTGGCGGAAAGTTATTTAAC60.5277044854881267No Hit
TTTTAATTTATATATACATGAGGGCTAATCATTATAGACCTGCATTGATA40.3518029903254178No Hit
GCGAGGAATATTAAACAATTTTCCGAAATAAAAAATAACTTTAACGAAAA40.3518029903254178No Hit
ATTTTAACTCATTGGTAATGGACATCATGCAAAATTGTCAATGTAAAGGA40.3518029903254178No Hit
GTCGCCGTGGGAAGGGATTGAGTTGAGGCGGGCTGGAATTGACTCATTCA30.2638522427440633No Hit
TTTTCAGTTAACCCCACAAGGTCATCCACAAAAACCGTTTAATCTAAACT30.2638522427440633No Hit
CTTGGAACCAACTGTTGTCGATGAGGTTCGCACTGGCTTGTATAGGCAAT30.2638522427440633No Hit
GGATTGTTAGGGGGTTTTGGGAGAATTGTTTGTACTTCTAGAGTAGGACT30.2638522427440633No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.2638522427440633No Hit
TTTCAATGTTCCAGCCAGTTGCATGACGGGTCCTATTTTACCTAGTTTAC30.2638522427440633No Hit
GTTGAGTTTTCCATTTGTCGTGCACAAGATGTGACGGATATGGAAACCCA30.2638522427440633No Hit
TAATACTTATAATTCTATATACAGTTTCATTTTTTGATATAGTTCTGTAG30.2638522427440633No Hit
TAAAACAACCAGATAGTTTCGCTTTGGATAATGTCGGAGCGAAGCAAACT30.2638522427440633No Hit
AGCAGATGGCCATGCTTCGAGCCATGCAACAAGGTGGTAATCAAATGAAC30.2638522427440633No Hit
ACTGGATCCATCTTGTCACCCAACTACACGGAAGCTGCATCCATCACCTG20.1759014951627089No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCACCCCCCCCCCCCCCCCCCC20.1759014951627089No Hit
GATAGATAGGACTGTATAGAAGACATTTCGGATGAAATGTGTCCATTCAT20.1759014951627089No Hit
GAAGAAAAAGACCAAAACGGAAGAATCAAAAGCTGAAGAAACGTCCGAGG20.1759014951627089No Hit
GGATTTGGCAGTGGTGGATCTGGCAGCAGTGGATCTGGCAGTGGTGGATT20.1759014951627089No Hit
GATCGGAAGAGCGTCGGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC20.1759014951627089Illumina Single End PCR Primer 1 (100% over 34bp)
TTTTTATCTATTCTGTTGTGTTTTTGTTGGAAGTTAAAAAGCTTTGTTTC20.1759014951627089No Hit
ATTTTACTTAAATCTGGACTCATGTCATGAAATAAAAAGTTATAGAGGAA20.1759014951627089No Hit
CCTGTGTCTACTTGTCCTTTTGCCTTTGGTGTACTGTGCCAGTATTGATG20.1759014951627089No Hit
GTGACACATACAGAAATAAACTGCTGTAGATAAAAAAATATATAATACTC20.1759014951627089No Hit
ATCGAAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT20.1759014951627089Illumina Single End PCR Primer 1 (98% over 50bp)
TGGATACCCAGCCCAGCCAGGATACCCACAGCAGCCTCCATATGGCCAGC20.1759014951627089No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)