FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-ctenidia-RNA-8_S59_L008_R2_001.fastp-trim.20220908.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-ctenidia-RNA-8_S59_L008_R2_001.fastp-trim.20220908.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4154
Sequences flagged as poor quality0
Sequence length35-151
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1493.5869041887337505Illumina Single End PCR Primer 1 (100% over 50bp)
TTTGTAATGACGGCTATCAGACAAGTAACGGAGCATGTACAAACATTGAC421.0110736639383726No Hit
GGCAAGTGTTTTGTAATAAGGAAGTTACATACTCATTTTATTTTTATATA370.8907077515647569No Hit
GTGAAAGAGAACAACCGGTGCTGTTACAGTGTCCAGATGGCAAAGATATA330.7944150216658643No Hit
TGTGAATTTCATTTTTTTGTGCTGTTTGTATCAGTCCTATCCATATATGA330.7944150216658643No Hit
GGGAATTATGTTTATATTAACTGACAGAGTGGAAAACAGTGCAGGAAGTG310.7462686567164178No Hit
GTTGACCTCCAGTAGGGGTTTTGCCCATTAATGCTTTTGGGGTGCCGTTG300.7221954742416947No Hit
GGAGAATTGGCATCTCACTCTCGTGCATTGCTACCCCACCCTGAACTGTC290.6981222917669716No Hit
GGTGGCAGTTATGTGATCACACAGAAATGGATCCATCAGTTTGATGTCAT290.6981222917669716No Hit
TGGAACTCCAACCGCTATAGCTAAAATAAAACAAGACACTCAGAAAGCAC290.6981222917669716No Hit
TTCACACAATCCTTCCCAGTCATCAAGCCTGCTTCATAAATTAGATAACT260.6259027443428021No Hit
GGGACACACAGTGATTTTGCTAAATTTTCACTAAACACAGCCACTGTTTT260.6259027443428021No Hit
GTCATTTTTGTGCCATCGACAGACGGTCAGTTAACCTAACCACTGTTTCC250.601829561868079No Hit
TGTATTTGCTGAAGTATCAAGATGAAGTTCTCAATCGGTTTACTGTTTGT250.601829561868079No Hit
AAACCAGAACTCAGAAAAAAAGTTATGCCTGCTGTGGTAGTGCGGCAGAG250.601829561868079No Hit
CGCGATAGTGAAATAGAAATGGACGATTCGGCGTCCGTACCGGTCATAAA250.601829561868079No Hit
TTTGATCTCCCTAGTGGAGATATTTTGTTACTCACCTTTGGTTTAAACAT250.601829561868079No Hit
GAGTGAACTATGAGGGAGTCAATCCGAGTCTGAACTAGTTACATGCACAC240.5777563793933558No Hit
TAATATACAGCTTCAAAAGTTAGCGGAAATTGAAGAAGAGAATGAGTTAC240.5777563793933558No Hit
TTTGCATTTGATATAAATGTCATTTTTCAGTATACATCAATATTAACATG240.5777563793933558No Hit
TGAAAACGCCTCTTTTAATACACATTCAAATGGTAAGAGTGACTATAGTT230.5536831969186327No Hit
CCACAAATAATGAAGACGATAGTAATACGGCATCACCTGGTCCACAAACA230.5536831969186327No Hit
CAGTGTATTAAGTTAAGTTACACATTATTAAATCCAGTTTGGACTATTGT230.5536831969186327No Hit
GTTGGAATTATTTGGACATTGTATATAAAGCTGTCAACAATACAATTTGT230.5536831969186327No Hit
AAAACTAAGAAAATAGAAGGAAACTAAGAAAATTAGAAGGAAACTAAGAA230.5536831969186327No Hit
GAACGTTTGTTTGTTCTTTATTTTGCATTTTCTTGTACAGATCTAAACAT230.5536831969186327No Hit
TATTGGGGTAGTGTTAAGGATTAGTGTTTTTGTGAGAAGAAAAACGGTAA230.5536831969186327No Hit
CGAGACTAAGCAAAGTACCAAATCACGTTGGATAATTGGAATAATCATTG220.5296100144439095No Hit
AGGATCTGTACGCAAACACCGTATTGTCCGGCGGTTCGACCATGTACCCA220.5296100144439095No Hit
TGTCATTCCATACAAAATGTCGTACCAGACAATACAGCCAACATCATACC220.5296100144439095No Hit
TGAAAACCCTGTTTGGTATTGGGATGCTTCGTGTATGTGTATGATTTGCA220.5296100144439095No Hit
GAGCAACAGTCGCTTTCGCATCGGGTCAATTAACAAGTTTATCACAAACA220.5296100144439095No Hit
GGAAACGACACCGTCTTGCTCTTAAGAGACGTCGTTCCAGTAAGAAACGT220.5296100144439095No Hit
GGGGAGCTTACTTTGTTCCAGGTGTATATAAAACAATATAATTATTATGC210.5055368319691863No Hit
GTATAGACTGTTGGCTAATCACGTCAGAGAGAGCGGGTTCGATCCAAGCG210.5055368319691863No Hit
CTTCCATAAAGCGATATCAATGGTGATACCACGTTCTCTCTCAGCCTTCA210.5055368319691863No Hit
CCTCAAATTCACTGTTTTATTATTGTGTTAAAAGTATATATATATGAACA210.5055368319691863No Hit
AAATATTAAACTTTATTGGTAGTTAATGAAACTAATCTTCATCAAGGAAT210.5055368319691863No Hit
TCATTATAAAATGCAAGGCAAAAAGATAACAAATGACCATATCACTTCTT210.5055368319691863No Hit
AAGATCAAGTTCCCACTGACCCACAGAATCCAAAGGAAACTTCACGCTCC200.4814636494944632No Hit
CAGGAAGCCACAGAGATGTTGGTAGGCAACGCGCAAAATCTCATGCAGGC200.4814636494944632No Hit
GTGGTTTCAATGGATAGATCATCACATCTTCTTTTGTCATAAAACAATAA200.4814636494944632No Hit
ACAATATGTACATGAACATGCATTTATAATCAAATTACTTTTTTTCTTTG200.4814636494944632No Hit
GTATGAGATATATAGTAAATCGATGTTGCACACAGATTCACTATTAAATA200.4814636494944632No Hit
TTCTGATTTACATCTGTAGACAAAGGGATGACTGCTCCAATAATGGTGTT200.4814636494944632No Hit
AAGAACAACAGCAGCACGAGGAGCCGGAGACAAATGAAAACCCTGACACC200.4814636494944632No Hit
CGAAGATCCACATTTAAAATATTTTAAACATTGCCTGGTGGTCTGTAAAG200.4814636494944632No Hit
CCCTGCTCTGTCACAATCTCTGTGATTGTTGATATACAACAATGCATCTC190.45739046701974No Hit
TGAAAATGACAAGTTGTTTTGATGTGTATTTGTAAAAACAAAACAAAGTA190.45739046701974No Hit
TTTTGTTTTACACTGATGTTACAAGGTAAAATGGACCATTGTTCTGTTTT190.45739046701974No Hit
CTATAGTTAATAACCCGATGCCATCTCGGAGTGTGTCACGTTTTTCATCT190.45739046701974No Hit
GGTTTTTAGGTGTTTTATGAATTCGAAATATTTCTCAGAGAATGAATTAA190.45739046701974No Hit
TGAATGTTTGGTGAGATGTTTACCTATATGTCATGAATGTTTGGTGAGAT180.4333172845450169No Hit
AGTATTTCCACTGTATAATTATTGCAAAATCGGAAGAAGTATTGTAATAT180.4333172845450169No Hit
TGTAATGAACCACGGACTTAAAGAATGTGACCTGTTTCTCACTAAGAAAA180.4333172845450169No Hit
AAAAAATGCAATTACTTATAAGTTGGCTGTTTCAATATTTTGAAGTGGAA180.4333172845450169No Hit
GTTAAGTTGTACATGAAGTTTGTTACATGTAGTTTGATACATGTAGTTTG180.4333172845450169No Hit
TTCCATTCCATTTCTGAAATTTCACATCTAACAGAAATGTTGAAAAAAGT180.4333172845450169No Hit
AGATTCAATACCAGTGCATTTGCATATACTTAATATAATGATATATACCA180.4333172845450169No Hit
TTTGCCATCTGTGGGGCCATAAGAAGAATGGGAGAATCAGATGATTGTTT170.4092441020702937No Hit
TGGAGTCTGGTTGAGATACGTGTCCAGAAGTGGTACACACAATATGTACC170.4092441020702937No Hit
GCCTTCCCTCACATTCAGGCCTTTCAGGCCTTTGATTTTATTTCTTACCA170.4092441020702937No Hit
TCTGTGCCTGCTGGCTGCAGCGGGCATTGTTGCCAAGGTTACATGCTTGA170.4092441020702937No Hit
GAATTGAGGTTGAAAGGTCACCAGAAGGAAGGATATGGTCTGTCGTGGAA170.4092441020702937No Hit
GGTAGGGTAGATACAGCCTTTAACAGATTTATTTTGCAACATTGTATTTT170.4092441020702937No Hit
CTCGCTTCTGAAACTTTGAATAAATACAATTCGCTGCTGAACTTTGAATA170.4092441020702937No Hit
CATACAGTGCCTTGCAAGGTAACTGGAAAGTGTGGTAGTGTAACTGTACG170.4092441020702937No Hit
TTCAGATCTCTTTAGTGTATATAAATATCCCTAAACCAACTATGGATCAT160.38517091959557054No Hit
CGGCCATTCACAGGGCTCAAAGGCCAAGCCGCCCCGACAAGGCCAGACGT160.38517091959557054No Hit
TTTGAACCTACCAGAGCTGGGACAACTACCTGCACTGAATTTGGAAGATG160.38517091959557054No Hit
AATTGAGGATCGTCATGACAAACCTGGGCGAGAAACTTACAGATAAAGAA160.38517091959557054No Hit
ATCACCATGCCGTCAAACCTGTGTAAGCAGTCACCTCTATTAAGCAGCCA160.38517091959557054No Hit
TAAGTAGCCGGTACAGTGTCAAACACTCACTTGGGGAGCATCCATCTGTT160.38517091959557054No Hit
GGAATATTGCGAGTCGGTGGACTACAGAGGCAGACCTATTGGAGAATATG150.36109773712084736No Hit
TGCGTATCTATGGAGTAAAGGGTTAAAAAGGTTACCAACAACAAAATATT150.36109773712084736No Hit
GGAGACCAGAAAATAAGCATGATATCAACTAGAACAAAATTATGTATTTT150.36109773712084736No Hit
TTAAAGTCCGAGTTACATCACAAGGCGACCAGACTAAGGTTTGGATTAAT150.36109773712084736No Hit
CGAAATCGTTCACATACAAGCCGGACAGTGCGGAAACCAGATCGGTGCAA150.36109773712084736No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT150.36109773712084736Illumina Single End PCR Primer 1 (100% over 50bp)
CTATTGTAAACAATTCTAAAATTCATTGCACACTGCGGTTCATATTGCCT150.36109773712084736No Hit
CACTCCGACTGCGCCTTCATGGTCGACAACGAGGCCATCTACGACATCTG140.33702455464612424No Hit
AACCATTGAGGAGGTTGATTAATTAAGTGTATGGTTAACTGAACATTAGA140.33702455464612424No Hit
TTTTTTAAATGTTGTGTGCAAATAAACTGATATTTTTCACATCACTGGTA140.33702455464612424No Hit
GGCTACCAGAAGCGATAGGCAGGTCCTGTCTGAGCGTGGGTCTCTTTTCA140.33702455464612424No Hit
GCGGTTTCGAGCTAGCAATTACCAGAGTACGACTCGAGTTAAGCCATTCA140.33702455464612424No Hit
AACATAATATACGTAGATATGATATCGTGCGTTTCACAACTGATCCCAAT140.33702455464612424No Hit
GTTTTACCCATCTGGCCCTGCTCTTTCCAGCTGGTCATTATGCCAATCTT140.33702455464612424No Hit
GTTGAGCATGGTTTAAATTAGACGTGAAAAATCAGTTTGCTTAACTTTAA140.33702455464612424No Hit
GTTGAAGTTCATTTTCTTATATCTTTATTATCTGTGACAAAAGGTGTCAA140.33702455464612424No Hit
CTTAAAAAGAATCCTCTCAAGAATAAGCGTGTTATGGACCGTCTAAATCC140.33702455464612424No Hit
GTTGGTAACTCACATCAGAGGGTTTGATCCCCAGCTCAATCCGATGCCAA130.31295137217140107No Hit
GACAAGATACGCATCTGAACCAGAGAACCCCACAAAATCGGCCAAGGCCA130.31295137217140107No Hit
GAATGTTCCATAACAAGTTGTTGATGAATCAGGAATTAGGGAGATCAAAG130.31295137217140107No Hit
CAAGATCTGGGATCTTGAAGGCAAGGTTGTAGTGGATGAGCTGAGACAGG130.31295137217140107No Hit
AAAAAAGCAAACAAACCAACATTGATTGCCAAGTCCAGTTTACTTATTGA130.31295137217140107No Hit
TTCAGGGCCCTGTAGACCCATTCAGACAGACGATGAAGACAAAGTCAAGA130.31295137217140107No Hit
CCAGCTCAACGATACAATTTCATTGACAGTCAAAATCAATATCTTCCAGA130.31295137217140107No Hit
CAAAACTGAATTTCACTTTTTATTAGTTTGACAAAGATTTAATTTAATTC130.31295137217140107No Hit
TGAACAGACTGATTGCTCAGATCGTATCCTCCATCACTGCCTCTCTCAGA130.31295137217140107No Hit
TTGCAGAATACCACCAAATGAATATGGACAACCGAAGGGATTTGCGTTTG130.31295137217140107No Hit
CTGACATTACAGACCCATGTGAAGAGTGTGTAATATGTATACACGTGTAT120.2888781896966779No Hit
TTGATATTTGTCAATGTCGGTCAAATCTTGCAGGAGTTATGGCTCCTTTT120.2888781896966779No Hit
GTAGAGGCTGATATGGAATACCTGGAAGAAAATATTCCAGCAGCATCTTC120.2888781896966779No Hit
GGGTGTCGTAAATTGTACTGAAAATGGGGTTGATGTTTTTTTTCCTGGTG120.2888781896966779No Hit
CATGCGCTGACGGTGAAAGTGTAACAAATTAATACTATTTATCTTTGTCT120.2888781896966779No Hit
TGGGGACGCGTCAGAAAAAAAAGATATGTCGTGGGTGTGTCACAAGTCAG120.2888781896966779No Hit
TCCAGACCAACTTGGTACCATACCCACGTATCCATTTCCCTCTGGCCACA120.2888781896966779No Hit
GAAAAGCATATTAACACAAATTCATCGTGCACAACGTCAACGAGTAGCAG110.26480500722195477No Hit
AACAGAATGGCGTCACCAAAATCCGTTTTTTCAAATGATATGTTTACAAT110.26480500722195477No Hit
GGATGCCTGGTTTGTTACGTTCATGACTTTGGTGTTGATGTCATGCCTTT110.26480500722195477No Hit
ATGCACCCTGTTTGAAGGAAGGGGCAATTTTTTTTAATGTTCTTAGGAGT110.26480500722195477No Hit
GTCAGAATCCCGCCCAGAATGCCGTAACGATACTCTGTGCACCGCCGTCG100.2407318247472316No Hit
GTTAAAGCGAGTTAGCTTATTTGTATGGTCAGTTGCTATCACTGCTTTTT100.2407318247472316No Hit
TGGGGGGATTCAGGATTCCCGTTTTTTTAGCGGGTTGTGGTTTAAGCTCC100.2407318247472316No Hit
TCTCATTGTAAGGTTTTACTTCTGTGGGTAATAGTTGAGACTTATCTCAT100.2407318247472316No Hit
GCTTCCTAGAGGTATACTATGGCGGATTAAACCAGTTGAGTTATTTATAT100.2407318247472316No Hit
CTCCAGTGTGGCAGCGTTCAGAATACTGTCTCAAGTAAACACTGGCGCGA100.2407318247472316No Hit
TACCAACTTCCAACTGAAACAGAACCAGCCCACAATCCCGGAGCCAGACT100.2407318247472316No Hit
ATGGCCTGCACTTTTTCCCCTGAAAAATAAAAAATATATATAATTTAATT100.2407318247472316No Hit
TGACCTTTTCCTTCACTGTCTAAAACCTGAAATGCTCGAAGAATCTGATC90.21665864227250844No Hit
TAAAAGACGGGCAATCTTGTATCGTTGTCGGAACAGAATCTGATTGACTG90.21665864227250844No Hit
AACATGCCAAAATATATTTTTAATAGAGTGGTAAAATTCAAACGAAATCC90.21665864227250844No Hit
TTAAATGATAGCTTAAATCAGATTTTTAACAAATGTATATTTGTTATCAT90.21665864227250844No Hit
TCCACGAATTCCTGTATGGCTAGGAAGCCAGCAGAAAACAATAGTATTAT90.21665864227250844No Hit
GGAAAATGTAAAACACTACCAAAATACTATGTGACAAAGGTTGCCGATTA90.21665864227250844No Hit
TGGTGATGATGATTTTATCTTCAGGAACGAATAATTGCTGCATAATGTGC80.19258545979778527No Hit
CAAGGATGAAGATGAACAGCAAAAGGATCGTATCCAATCCAAGAATGGTT80.19258545979778527No Hit
TATTGGTTTTATTGTATTTTCAACTGTTGTTAAATTTGCTTTTTTTTATC80.19258545979778527No Hit
TCATGCGAATGACTTTGGTCTGTTCTTCAAAAATCAAGGTCACATATCAG80.19258545979778527No Hit
CTTTAAAAAACTTTTTTTTTTATTTTTTCATTTTAAGTTTCCGTCGGGAC80.19258545979778527No Hit
TTTAAAATGACTAGTAAAAATCAAAATCTGGCAAAATAGATAAGCTTCCT80.19258545979778527No Hit
CAAACACGGACTTTGCTATCAGTACCACAGGAGTAATAACTGTCGCAAAG70.16851227732306212No Hit
TTGAGAAACTGTGCAGTGATTATCTCCCTTTTTCAGAGCGACCAGAATCG70.16851227732306212No Hit
AAGTTTGGATGACAGTGGGTTCTGTTCGATGAAGCTATAACAGTTTAAAG70.16851227732306212No Hit
CGTGTTTCTATGGGGATTCTATGCATAACGCGCAACTAGTACAACTTACA70.16851227732306212No Hit
TTTAACTTCTTTCATTTGGGACTCTCTATGAATGACGAACAGTCACATTT70.16851227732306212No Hit
AACAAGCAGAAGACGGCATACGAGATTACAAGGTGACTGGAGTTCAGACGTGT60.14443909484833894TruSeq Adapter, Index 12 (100% over 51bp)
GTATTGAAACAAAGGAAGACAGATTTGCTTTACTTGGTGGCTTGAGTAAG60.14443909484833894No Hit
CCTCAAATTCACTGTTTTATTATTGTGTTAAAAGTATATATATGAACAGA60.14443909484833894No Hit
AAACAATATATTTTTAAGGATGGCCATGTAAAACTGGTGGAAAATAAATA60.14443909484833894No Hit
TGATCCTAAAGAGATTTACAACGAGGCCCAGGAACTAGAAGAATGAATGT60.14443909484833894No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.1203659123736158No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC50.1203659123736158No Hit
CTGTTTTATGTTTGTACATTTAAGAATTGATTTTCAATTTTCAGGTTGGA50.1203659123736158No Hit
ACAAGTGTTCCTTATTCACAAAGGAACTTGCTATGTACAATATATTAAGC50.1203659123736158No Hit
GGGAGTTTCATCCTCTAACTTCCCTGAAACCAAATTTACAAATGTGATCA50.1203659123736158No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)