FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-ctenidia-RNA-8_S59_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-ctenidia-RNA-8_S59_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4590
Sequences flagged as poor quality0
Sequence length35-151
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC1864.052287581699346TruSeq Adapter, Index 12 (100% over 50bp)
AGAACATTCATTTTGTCCGCTACATTCATCTATGTCATCACATGCATTGC390.8496732026143791No Hit
AGAATGACGAACAAAAAGCAATCAGAAAAGCTCACGTAAGCGTTCAGCTC370.8061002178649237No Hit
CGACTTTACAGAAAAGACTTATCTGAAAAAATAAACATACCAAATTGTTC360.7843137254901961No Hit
GGAGCCTTCAATATCCTGGTGACAATCCTAATGTGAACCTTTGCAAATGT330.7189542483660131No Hit
AAAGCACTGCGCTTTTTAAAAAACCGAATCGCATAATGACTTCACGTCAC300.6535947712418301No Hit
AAGAACCTTAAATTCACAATAATACAGTGAACTCAGTGATAACTGTCTCT300.6535947712418301No Hit
TTTATCAACATCTTTACCTAAGATTTTTTTATTTAACTCAGTTCGTTTTG300.6535947712418301No Hit
TTTTGTTTCTATACAGGAATATACATTTTAAAACACACACACATATACAT280.6100217864923747No Hit
AGAACAGACCTGCTCCACTGGACAGGGTTCCAAAAGGCATAGATTGGCGT270.5882352941176471No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC270.5882352941176471TruSeq Adapter, Index 12 (100% over 50bp)
TTTTTTTCCAGAAAGGTCACAGCGACAAACTTGGTTGTATTTTTTTCGAG260.5664488017429194No Hit
CGAGGTCCACCAACGTCGTCAAACCCAGCTTTGTTGCAAGTTCGGGGATC250.5446623093681917No Hit
GAGGGAAATTTAGTTCCGCATTTCATAAAGTACACGTTTCTCACTACTTC240.522875816993464No Hit
ATCAATCCTACTGTCCCCAAACAGGTTTCAACCAGTTGAGGACTAACAAA240.522875816993464No Hit
GTCTGAATTTGACGTTTCTGTCAGCATGCAACTAGATGGCGTTTCCTGTT240.522875816993464No Hit
GAATGTTTCGCTCTAAGATTCCCTCATCTTGGAGCTCCTCCAGTTTGTTT240.522875816993464No Hit
CTGGTCCAGTAATGGCTGACCCTTTCATTTCACCTTTATTATTTACTATG240.522875816993464No Hit
CCTTACATAAAGTTTTATAAAATACATTGGTGAGATATGGACCTGATGGT240.522875816993464No Hit
AAGTTTTTTGCATAATAGATGTATGAAGTTAAATTTAATGTTTCTTTCAC230.5010893246187363No Hit
GGGCATATTTATTTAATAAGCTGAACTTAAATTAAGACTTACTTAAGCCA220.4793028322440087No Hit
ATGGAACCTCCGATCCAGACAGAGTACTTCCTCTCTGGTGGGGCAATGAT220.4793028322440087No Hit
CTATTTCTTTAGTTTCCTTCTATTTTCTTAGTTTCCTTCTATTTTTTCTA220.4793028322440087No Hit
TGTGGCAACAACTCGATTCGTTGTGCTGTTCATGCCTTTGGCGGAAAACG220.4793028322440087No Hit
AAAAAATGCAATTACTTATAAGTTGGCTGTTTCAATATTTTGAAGTGGAA220.4793028322440087No Hit
AATTTTCTCACATACTTTTTAGATCTAGTTCAGGTCAAACCTATGACCTC220.4793028322440087No Hit
AAACGATTAAGTTTAAATGTACAAAATGGGAAGAATAATTTATTTAACTG220.4793028322440087No Hit
ACACCTTGTATCATCAGTGTAAAACACAACAATGGTCCATAATACCTTGT220.4793028322440087No Hit
AGAAACTCAATATTTTCCATATACATGCTTGTATGTGTGTCATTTTCGGA220.4793028322440087No Hit
GTTTATTGCCAATTTTCCAATTGAGGAAAATCCTGATATAAGTGTTGTAA210.4575163398692811No Hit
TATACATGTACAAATTCAAACACATTTCTCTAGATTTTCACAAGAGATAC210.4575163398692811No Hit
TTAGACTTAGGTATATTACTACATCGTAGTGTCGCGTGTGCCTTTAAGAT210.4575163398692811No Hit
CAACGTTCTCGTTCGCTGGGAGCAAACGTTCGTCAGAGCCGGTGTCATCT200.4357298474945534No Hit
AGAAAGCGCAGAGCGTATATGAACCGCCCTCGTGTCCGTTTCCTGGGTCG200.4357298474945534No Hit
AAAAGAGTCAAGGGGGCATAACTGTGACAGCCAGTATGCGATTAGTAAAA200.4357298474945534No Hit
ATTCTACCCACCCTAAATCCAACGTCAAAAGTCTACTCCTACATCTCCCC200.4357298474945534No Hit
TGAACTAGATCGGCTGTGGTCTCTATTCACAGAACCATTGTAAATTATCA200.4357298474945534No Hit
TGAACTTAAATTAAGACTTACTTAAGCCATTGACAAGTTTTTCCTTCTGA200.4357298474945534No Hit
GAACCATTGAGAAGTTCGAGAAGGAAGCACAGGAGATGGGAAAAGGATCC190.41394335511982566No Hit
TAAATATTCTTCAATATTATACTTGTGAAATAAGAGCATGCATTGTTTTA190.41394335511982566No Hit
GCCCTCTCGCCCTTCACCTGCCAGGCCAAGGTGATGAGCAGCATCAAGGG190.41394335511982566No Hit
TCAAAACCTGAAGACCTTATTTGGAAGCATTTTTCACCTTTCAGTAAGGT190.41394335511982566No Hit
TAATTTCAAAACTTCTAAACTCGCGTTACAATGGTGACTTTTGCATGAAC190.41394335511982566No Hit
GTAATGTTATTTGATAAAAATCGGCACTTAATAATATAATGCAATAAAGT190.41394335511982566No Hit
TGAAAACGAGAAATTGCGAGATTTTGTGTGATTACGGAGTGTTCACAAAA180.39215686274509803No Hit
TTTCACACTTATAATGGTTAGCAAAATCATTTTTATTTGTACTTATAAAT180.39215686274509803No Hit
CTGTTGACATATAATTTACAAACTAAGACTTTGACTGAGATTGTGACATG180.39215686274509803No Hit
AATTTTGTTAACTGCTACGGCTGACGTGATCACTTCAGTCGGGCCCAGGG180.39215686274509803No Hit
AGTCATTTGTGTTTCGACATTATCCGGTTTCACATCAACCGATGGTGGTT180.39215686274509803No Hit
TAAAGACCAGCATCTCACCAAACATTCATGACATATAGGTCAGCATCTCA180.39215686274509803No Hit
TCAATAAACTTCATAAATTTTGTTTTGCTTGCTTCCTTAAATTTGGCTTT180.39215686274509803No Hit
CCCTGATCCCCAATCTAGGTCTTCTGTCCTAGAAACAAAACAGACTCAAC180.39215686274509803No Hit
CAAAACTCAAAATAATCTATTTTGCCATCCCCGTCAATATCAGCTTCTCT170.3703703703703704No Hit
CAAAGTTTCAGAAGCGAGTTGTATTTATTCAAATTCAGCAGCGAATTGCA170.3703703703703704No Hit
GTCATTTTTGTGCCATCGACAGACGGTCAGTTAACCTAACCACTGTTTCC170.3703703703703704No Hit
CATTAAAGCAATATGGTTAGGAGAAGCAAAGCCATGAGTGTGCATACCAG170.3703703703703704No Hit
CTTCCTTAGGACTTGTATTGATATCCCATAAGCAAATTGTATGGTCATCT170.3703703703703704No Hit
GAAAGGACTGGGTCAAAGCTCTACATCGCATTATGCAAATGAAGTAGGGT170.3703703703703704No Hit
AGTTAACCTACCCCTGTTTTCTGTGATCCTGGAAGTTAACCAGTATTTGA170.3703703703703704No Hit
CTATAGTTAATAACCCGATGCCATCTCGGAGTGTGTCACGTTTTTCATCT170.3703703703703704No Hit
TGTGGCTTCGTTGTAGTACACATTTATTCTTTCCAGCTGCAGATCTGAGT160.34858387799564267No Hit
TTTATACCTCGTTCTCAAAATATTTAAGCCTCGTTTTCAAAAATGAAACG160.34858387799564267No Hit
TTAAAAGCTAAGGTTTATTTGATAAAAATAGAAGCAGTTTGTGAACATAA160.34858387799564267No Hit
TGAGAAGGCCTTTATTTCACCTATAAACAGATTACAGTAAATTAGAATGT160.34858387799564267No Hit
TTATACAATAACAAGCAGTAAAATACGATTTAACTTAAAAAGTATTTAAC160.34858387799564267No Hit
TTTTTTTTAAGTTTTTCTCTTTATTTTCTTATTTTTGTACAAAATAAGAA150.32679738562091504No Hit
GTTCTTCTCTTGAACCATATTATGCTCCTGACAGAATTCTCAATTTTAAT150.32679738562091504No Hit
GTCATCAACAGTATCACTTATGAATTATACTCCCTGTTAGAGAAGGATTT150.32679738562091504No Hit
GTGTACCACGGACGCTTCCTCAACCAGCGGATGGTGTAACCCGAGTCTAC150.32679738562091504No Hit
GGTACAGGTGCACTTACAATTCCTGTACCACGTGGGGCAGGAATCAGACG150.32679738562091504No Hit
TTTACGTCCTCCACGGCGAACACGCACACGGTAGACAACGTAACCTTGCT150.32679738562091504No Hit
TTTTTTAATAATAATAACGGTTTTATTCAACGAAGAAACTCAGTTGGACG150.32679738562091504No Hit
GTAAAAACATAATAGGGAAAAAATATCTCGATTGATGGACTGGTTTACGA150.32679738562091504No Hit
TTGTTCTTCTTTATCATTTCCCACTTCTGACTTGAGCATATTTGTTAACT140.30501089324618735No Hit
GTATATACAATGATTAAAATGATAATTGTTTTGATGCTGTTATTTTTTGT140.30501089324618735No Hit
GCCATGTCCAATGTTAATACATAATTGCATAATTTTCAATGTGATGACAT140.30501089324618735No Hit
AGCATTTACACTTGTGTCTGGAGCAATGACAAAGTTTGCGCCGTGGGTGA140.30501089324618735No Hit
TTTTTACTTTTAAATATATGTTTATTTTTTATGGCGAATATATAAAACAT140.30501089324618735No Hit
AAATGTAATAGTATGGTGAGATCCTATTGGAAATAAATAAACCAAGCTAC140.30501089324618735No Hit
AACATAATATACGTAGATATGATATCGTGCGTTTCACAACTGATCCCAAT140.30501089324618735No Hit
GGTCTGGTATATGTATGCAATTTACCATGGATAGATTTTGTAGTTAGGAC140.30501089324618735No Hit
ATTACACCGATGATAAATAATGATTTCTTTTCAACGGGGAAGGAAAGAAG140.30501089324618735No Hit
GGCTGCTGCAGGACGTTTAGCATCGACAACACCTGACCTGGCACGCTTAC140.30501089324618735No Hit
GGATACTATATTTTTGAGGGCTTCTATTTGAGCTAGAAGGAATTGGAGGC140.30501089324618735No Hit
GTATGTGGCCAGAGGGAAATGGATACGTGGGTATGGTACCAAGTTGGTCT130.2832244008714597No Hit
TTGGTATCGCAGATAATGTTGGGGTCGCTACATCGTGATAATGCCTGGAT130.2832244008714597No Hit
GTAACGTTCTTAAGGTAACGCACAGCACGCTTCACATGCATGTGTTTAAT130.2832244008714597No Hit
CTTGGACCTCGTATAGTCGGGAGACAGACGCGCGAGTGTCCCACCAGTGA130.2832244008714597No Hit
GGACAACATCACCTCTGTACAACATACAACAGGCCATGTACTTGCCGTGA130.2832244008714597No Hit
AAAAAACAACACCAAAAACACTTCAGCTTTTTGGTGCACATGTATGTATA130.2832244008714597No Hit
ATTTAAACATCGTTCTTTTAACTTTTTTGGTTTAATTAAAACAATTAAAA130.2832244008714597No Hit
CTATCTGTAATTTTTTAATGCCATATCCAACTGGTACCAGTTTTGATGCT130.2832244008714597No Hit
TGAAAGTCTGTATTGATTATAATAAAAACTTCAATAACATTTTGTATCTT130.2832244008714597No Hit
CTGTAACATCGCTTTAGCATACAGAACAAGTCAACAAGATCTTAAATGTT120.261437908496732No Hit
CACATCGTCACGGTCGAAGTAGTAGGCCATAGACAAGTACACGTAGCTGG120.261437908496732No Hit
TACAAGCGACGAATTGTTCACATAAAATTAAAAACAAAATTACTTTTTTA120.261437908496732No Hit
ATTTAATAAACTATGATACAATTAGAAATGTGTCCATAGGGCACGGCTGC120.261437908496732No Hit
CAAAACTTATTTGACAGTTTTATATATATATATATATCTAGATATGGCAT120.261437908496732No Hit
TGAAACTTTACAATGATATAACAATCTCAACTGAAACCTTACAATGAGAT110.23965141612200436No Hit
ATAAACTCCCTAAATTTTCCTACTATTATTTCCATCCCTCATCCTCTAAT110.23965141612200436No Hit
GTTTCCGAGGACAACATTTTAGATTTTGATTTTCCGTACAACATTTTAAG110.23965141612200436No Hit
GGAAACTGGTGTAAGGTCGGGAATACCATACGTTGTTGTTACATTGTACC110.23965141612200436No Hit
AAACTATATTATAAAAGAAATATGCATCAGGATAATCAGAATACCGCCGC110.23965141612200436No Hit
CTTCATGTCCTCCATCCAGAAGCCAACACATGTCAGGATATTGTCAGCCT110.23965141612200436No Hit
GCATTCTCAGGGATTATGTGTGTATTTGTTTGTATATAAAACATACCAAT110.23965141612200436No Hit
AAAACCTTTATGTGAGAAACTTAGTGTATACTAATTCATTTTAGCTTGAC110.23965141612200436No Hit
GTGGGAACTTGATCTTGGAATCATGGAACTGTTTGATGTTGGGTCGTCTG110.23965141612200436No Hit
TTTTATACAAATCATCATTTATTTATGATATGAAACAAATACATGTTGAA100.2178649237472767No Hit
CCTGATTCCTCAAAACGTAAATTAATGTTGTTTTCTACTACCTCTTGTTC100.2178649237472767No Hit
GCGAATGCTAACTGGACTATTCTTCCACTGATGTCCAACCCATCTTTCTC100.2178649237472767No Hit
TGACAGTGCAGCTACTAATACGAATGCTACTAATAATGAGGAGACTATTA100.2178649237472767No Hit
CTGCATTGCATGGGGTGGATCTCGTTCTGGCCAGCGGTGGCTATTGTCTG100.2178649237472767No Hit
GTACAACATACAGCAGGCCATGTACTTGCCGTGACGTGGGTCACATTTCA100.2178649237472767No Hit
CGAATATGTAGCATTTGAGAAAGTATTTTCACGAGAAAGTGTTCCTGTAG100.2178649237472767No Hit
TTAACTTTGATCCACAAAATGTCACAATGGTTGCAATAACGTCAGTAAAT90.19607843137254902No Hit
GAGCCGATTACCAAACGGAGCCTCCACCTTTTCAGCTGAACTCAAAGCCA90.19607843137254902No Hit
TAATAATATTGTAATAGCCCCCGCCAACACAGGCATAGCAAGCACCAGCA90.19607843137254902No Hit
GGCCATTCTTATAACAACCAAAGGTTAGGTAAGAAAATTCTCCATTGTCG90.19607843137254902No Hit
CTTGCTTTCCTTTATATTCGTAGCACTGTTCTGTATCGATTCCATGGTTA90.19607843137254902No Hit
CTTAAGTTTACTCATACCCTCTATTTTCCTTTGAGAGAAAATGATGCCAA90.19607843137254902No Hit
ACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTT90.19607843137254902TruSeq Adapter, Index 12 (100% over 50bp)
CAAACTCGTCCTTTTCGGCAAGTTGGTTAGCATCCAACCAGCTAATAACT80.17429193899782133No Hit
AGATATGATGTGGTTGATATTAGCCAATGGGATGTACTCTGAATAAATAC80.17429193899782133No Hit
GGAAAACGTGTATCTATGGAGTAAAGGGTTAAACAGGTCAAACTGATGCA80.17429193899782133No Hit
GAAAAAGTCGCCAAGCACGGTAATCACATGTAAATGCCGCAAACTGAAAA80.17429193899782133No Hit
CAACTGGTTACATAAGATTTGAAAAGTTTTTACCAATGATGACAAAAGTT80.17429193899782133No Hit
CAAGAACTTTTTTTTTGTTCTCACAAAACTCAATTAACAACAATATAAAT80.17429193899782133No Hit
CTAAAATCTCATCATGCTTTTGCAGATAATTCGCGACATTTTACAAGGTG70.15250544662309368No Hit
GAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCT70.15250544662309368TruSeq Adapter, Index 12 (100% over 50bp)
GAACTTACAGGTTGCTTTATATATATATATATATATATTTCATTAAGAAT70.15250544662309368No Hit
AACAAACTTGTATCTATTATATTATTTATTACATTATTTACATATATATA70.15250544662309368No Hit
TGTTCCCCTAGATAGTCCTCTGTTGATCCAATAATACAACAGATAACGTG70.15250544662309368No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC70.15250544662309368No Hit
CGGAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTC60.130718954248366TruSeq Adapter, Index 12 (100% over 47bp)
TTGAACTGTAATTCAGCATCTTATTTGTAATAACCATCTCTCTTCAATTC60.130718954248366No Hit
GCTTAACTGTCTAGCTATGTTCGGATATTTTATGTGAAAACATTTCAAGT60.130718954248366No Hit
GCCACCTGCATCACGTCATGTACAATGTTCTCATACTTCGCTGGCACACA60.130718954248366No Hit
CTTGTACATCATAATTGCAGTGGATACCAGACTTAGAGACCCCTGCTGTT60.130718954248366No Hit
CTGAAATCAATTGCATTAATCATCAAATCCCGGATTACTGCTGCCGCATC60.130718954248366No Hit
GGGAACTGTTCTTCGTAGAATTTCCAGAATTTGGACTATTTTTATTCCTA60.130718954248366No Hit
CTTTAATGTTTGTTTGCTGTGCTCCGACCCAGTATTTTCCATGTGGGCAG50.10893246187363835No Hit
TACACGCGACGAATTGTTCACATAAAATTAAAAACAAAATTACTTTTTTA50.10893246187363835No Hit
TTTTAATATAACTTTAATCACAAACATTGTATACCACATTTGGCCACAAT50.10893246187363835No Hit
GATACATTCAATATTTAAACTAATCACATTTTCACACAGCCTTTATTTAC50.10893246187363835No Hit
CAAGAACCTTTTTTTTGTTCTCACAAAACTCAATTAACAACAATATAAAT50.10893246187363835No Hit
GCGGGAACTTGATCTTGGAATCATGGAACTGTTTGATGTTGGGTCGTCTG50.10893246187363835No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAAGCTCGTATGC50.10893246187363835TruSeq Adapter, Index 12 (98% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)