FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-ctenidia-RNA-8_S59_L008_R1_001.fastp-trim.20220908.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-ctenidia-RNA-8_S59_L008_R1_001.fastp-trim.20220908.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4154
Sequences flagged as poor quality0
Sequence length35-151
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC1403.370245546461242TruSeq Adapter, Index 12 (100% over 50bp)
AGAACATTCATTTTGTCCGCTACATTCATCTATGTCATCACATGCATTGC390.9388541165142033No Hit
AGAATGACGAACAAAAAGCAATCAGAAAAGCTCACGTAAGCGTTCAGCTC370.8907077515647569No Hit
CGACTTTACAGAAAAGACTTATCTGAAAAAATAAACATACCAAATTGTTC360.8666345690900338No Hit
GGAGCCTTCAATATCCTGGTGACAATCCTAATGTGAACCTTTGCAAATGT330.7944150216658643No Hit
AAAGCACTGCGCTTTTTAAAAAACCGAATCGCATAATGACTTCACGTCAC300.7221954742416947No Hit
AAGAACCTTAAATTCACAATAATACAGTGAACTCAGTGATAACTGTCTCT300.7221954742416947No Hit
TTTATCAACATCTTTACCTAAGATTTTTTTATTTAACTCAGTTCGTTTTG300.7221954742416947No Hit
TTTTGTTTCTATACAGGAATATACATTTTAAAACACACACACATATACAT280.6740491092922485No Hit
AGAACAGACCTGCTCCACTGGACAGGGTTCCAAAAGGCATAGATTGGCGT270.6499759268175253No Hit
TTTTTTTCCAGAAAGGTCACAGCGACAAACTTGGTTGTATTTTTTTCGAG260.6259027443428021No Hit
CGAGGTCCACCAACGTCGTCAAACCCAGCTTTGTTGCAAGTTCGGGGATC250.601829561868079No Hit
GAGGGAAATTTAGTTCCGCATTTCATAAAGTACACGTTTCTCACTACTTC240.5777563793933558No Hit
ATCAATCCTACTGTCCCCAAACAGGTTTCAACCAGTTGAGGACTAACAAA240.5777563793933558No Hit
GTCTGAATTTGACGTTTCTGTCAGCATGCAACTAGATGGCGTTTCCTGTT240.5777563793933558No Hit
GAATGTTTCGCTCTAAGATTCCCTCATCTTGGAGCTCCTCCAGTTTGTTT240.5777563793933558No Hit
CTGGTCCAGTAATGGCTGACCCTTTCATTTCACCTTTATTATTTACTATG240.5777563793933558No Hit
CCTTACATAAAGTTTTATAAAATACATTGGTGAGATATGGACCTGATGGT240.5777563793933558No Hit
GGGCATATTTATTTAATAAGCTGAACTTAAATTAAGACTTACTTAAGCCA220.5296100144439095No Hit
AAGTTTTTTGCATAATAGATGTATGAAGTTAAATTTAATGTTTCTTTCAC220.5296100144439095No Hit
ATGGAACCTCCGATCCAGACAGAGTACTTCCTCTCTGGTGGGGCAATGAT220.5296100144439095No Hit
CTATTTCTTTAGTTTCCTTCTATTTTCTTAGTTTCCTTCTATTTTTTCTA220.5296100144439095No Hit
TGTGGCAACAACTCGATTCGTTGTGCTGTTCATGCCTTTGGCGGAAAACG220.5296100144439095No Hit
AAAAAATGCAATTACTTATAAGTTGGCTGTTTCAATATTTTGAAGTGGAA220.5296100144439095No Hit
AATTTTCTCACATACTTTTTAGATCTAGTTCAGGTCAAACCTATGACCTC220.5296100144439095No Hit
AAACGATTAAGTTTAAATGTACAAAATGGGAAGAATAATTTATTTAACTG220.5296100144439095No Hit
ACACCTTGTATCATCAGTGTAAAACACAACAATGGTCCATAATACCTTGT220.5296100144439095No Hit
AGAAACTCAATATTTTCCATATACATGCTTGTATGTGTGTCATTTTCGGA220.5296100144439095No Hit
GTTTATTGCCAATTTTCCAATTGAGGAAAATCCTGATATAAGTGTTGTAA210.5055368319691863No Hit
TATACATGTACAAATTCAAACACATTTCTCTAGATTTTCACAAGAGATAC210.5055368319691863No Hit
TTAGACTTAGGTATATTACTACATCGTAGTGTCGCGTGTGCCTTTAAGAT210.5055368319691863No Hit
CAACGTTCTCGTTCGCTGGGAGCAAACGTTCGTCAGAGCCGGTGTCATCT200.4814636494944632No Hit
AGAAAGCGCAGAGCGTATATGAACCGCCCTCGTGTCCGTTTCCTGGGTCG200.4814636494944632No Hit
AAAAGAGTCAAGGGGGCATAACTGTGACAGCCAGTATGCGATTAGTAAAA200.4814636494944632No Hit
ATTCTACCCACCCTAAATCCAACGTCAAAAGTCTACTCCTACATCTCCCC200.4814636494944632No Hit
TGAACTAGATCGGCTGTGGTCTCTATTCACAGAACCATTGTAAATTATCA200.4814636494944632No Hit
GAACCATTGAGAAGTTCGAGAAGGAAGCACAGGAGATGGGAAAAGGATCC190.45739046701974No Hit
TAAATATTCTTCAATATTATACTTGTGAAATAAGAGCATGCATTGTTTTA190.45739046701974No Hit
GCCCTCTCGCCCTTCACCTGCCAGGCCAAGGTGATGAGCAGCATCAAGGG190.45739046701974No Hit
TCAAAACCTGAAGACCTTATTTGGAAGCATTTTTCACCTTTCAGTAAGGT190.45739046701974No Hit
TAATTTCAAAACTTCTAAACTCGCGTTACAATGGTGACTTTTGCATGAAC190.45739046701974No Hit
GTAATGTTATTTGATAAAAATCGGCACTTAATAATATAATGCAATAAAGT190.45739046701974No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC190.45739046701974TruSeq Adapter, Index 12 (100% over 50bp)
TGAAAACGAGAAATTGCGAGATTTTGTGTGATTACGGAGTGTTCACAAAA180.4333172845450169No Hit
TTTCACACTTATAATGGTTAGCAAAATCATTTTTATTTGTACTTATAAAT180.4333172845450169No Hit
CTGTTGACATATAATTTACAAACTAAGACTTTGACTGAGATTGTGACATG180.4333172845450169No Hit
AATTTTGTTAACTGCTACGGCTGACGTGATCACTTCAGTCGGGCCCAGGG180.4333172845450169No Hit
AGTCATTTGTGTTTCGACATTATCCGGTTTCACATCAACCGATGGTGGTT180.4333172845450169No Hit
TGAACTTAAATTAAGACTTACTTAAGCCATTGACAAGTTTTTCCTTCTGA180.4333172845450169No Hit
TAAAGACCAGCATCTCACCAAACATTCATGACATATAGGTCAGCATCTCA180.4333172845450169No Hit
TCAATAAACTTCATAAATTTTGTTTTGCTTGCTTCCTTAAATTTGGCTTT180.4333172845450169No Hit
CCCTGATCCCCAATCTAGGTCTTCTGTCCTAGAAACAAAACAGACTCAAC180.4333172845450169No Hit
CAAAACTCAAAATAATCTATTTTGCCATCCCCGTCAATATCAGCTTCTCT170.4092441020702937No Hit
CAAAGTTTCAGAAGCGAGTTGTATTTATTCAAATTCAGCAGCGAATTGCA170.4092441020702937No Hit
GTCATTTTTGTGCCATCGACAGACGGTCAGTTAACCTAACCACTGTTTCC170.4092441020702937No Hit
CATTAAAGCAATATGGTTAGGAGAAGCAAAGCCATGAGTGTGCATACCAG170.4092441020702937No Hit
CTTCCTTAGGACTTGTATTGATATCCCATAAGCAAATTGTATGGTCATCT170.4092441020702937No Hit
AGTTAACCTACCCCTGTTTTCTGTGATCCTGGAAGTTAACCAGTATTTGA170.4092441020702937No Hit
CTATAGTTAATAACCCGATGCCATCTCGGAGTGTGTCACGTTTTTCATCT170.4092441020702937No Hit
TGTGGCTTCGTTGTAGTACACATTTATTCTTTCCAGCTGCAGATCTGAGT160.38517091959557054No Hit
GAAAGGACTGGGTCAAAGCTCTACATCGCATTATGCAAATGAAGTAGGGT160.38517091959557054No Hit
TTTATACCTCGTTCTCAAAATATTTAAGCCTCGTTTTCAAAAATGAAACG160.38517091959557054No Hit
TTAAAAGCTAAGGTTTATTTGATAAAAATAGAAGCAGTTTGTGAACATAA160.38517091959557054No Hit
TGAGAAGGCCTTTATTTCACCTATAAACAGATTACAGTAAATTAGAATGT160.38517091959557054No Hit
TTATACAATAACAAGCAGTAAAATACGATTTAACTTAAAAAGTATTTAAC160.38517091959557054No Hit
TTTTTTTTAAGTTTTTCTCTTTATTTTCTTATTTTTGTACAAAATAAGAA150.36109773712084736No Hit
GTTCTTCTCTTGAACCATATTATGCTCCTGACAGAATTCTCAATTTTAAT150.36109773712084736No Hit
GTCATCAACAGTATCACTTATGAATTATACTCCCTGTTAGAGAAGGATTT150.36109773712084736No Hit
GTGTACCACGGACGCTTCCTCAACCAGCGGATGGTGTAACCCGAGTCTAC150.36109773712084736No Hit
GGTACAGGTGCACTTACAATTCCTGTACCACGTGGGGCAGGAATCAGACG150.36109773712084736No Hit
TTTACGTCCTCCACGGCGAACACGCACACGGTAGACAACGTAACCTTGCT150.36109773712084736No Hit
TTTTTTAATAATAATAACGGTTTTATTCAACGAAGAAACTCAGTTGGACG150.36109773712084736No Hit
GTAAAAACATAATAGGGAAAAAATATCTCGATTGATGGACTGGTTTACGA150.36109773712084736No Hit
TTGTTCTTCTTTATCATTTCCCACTTCTGACTTGAGCATATTTGTTAACT140.33702455464612424No Hit
GTATATACAATGATTAAAATGATAATTGTTTTGATGCTGTTATTTTTTGT140.33702455464612424No Hit
GCCATGTCCAATGTTAATACATAATTGCATAATTTTCAATGTGATGACAT140.33702455464612424No Hit
AGCATTTACACTTGTGTCTGGAGCAATGACAAAGTTTGCGCCGTGGGTGA140.33702455464612424No Hit
TTTTTACTTTTAAATATATGTTTATTTTTTATGGCGAATATATAAAACAT140.33702455464612424No Hit
AAATGTAATAGTATGGTGAGATCCTATTGGAAATAAATAAACCAAGCTAC140.33702455464612424No Hit
AACATAATATACGTAGATATGATATCGTGCGTTTCACAACTGATCCCAAT140.33702455464612424No Hit
GGTCTGGTATATGTATGCAATTTACCATGGATAGATTTTGTAGTTAGGAC140.33702455464612424No Hit
ATTACACCGATGATAAATAATGATTTCTTTTCAACGGGGAAGGAAAGAAG140.33702455464612424No Hit
GGCTGCTGCAGGACGTTTAGCATCGACAACACCTGACCTGGCACGCTTAC140.33702455464612424No Hit
GGATACTATATTTTTGAGGGCTTCTATTTGAGCTAGAAGGAATTGGAGGC140.33702455464612424No Hit
GTATGTGGCCAGAGGGAAATGGATACGTGGGTATGGTACCAAGTTGGTCT130.31295137217140107No Hit
TTGGTATCGCAGATAATGTTGGGGTCGCTACATCGTGATAATGCCTGGAT130.31295137217140107No Hit
GTAACGTTCTTAAGGTAACGCACAGCACGCTTCACATGCATGTGTTTAAT130.31295137217140107No Hit
CTTGGACCTCGTATAGTCGGGAGACAGACGCGCGAGTGTCCCACCAGTGA130.31295137217140107No Hit
GGACAACATCACCTCTGTACAACATACAACAGGCCATGTACTTGCCGTGA130.31295137217140107No Hit
AAAAAACAACACCAAAAACACTTCAGCTTTTTGGTGCACATGTATGTATA130.31295137217140107No Hit
ATTTAAACATCGTTCTTTTAACTTTTTTGGTTTAATTAAAACAATTAAAA130.31295137217140107No Hit
CTATCTGTAATTTTTTAATGCCATATCCAACTGGTACCAGTTTTGATGCT130.31295137217140107No Hit
TGAAAGTCTGTATTGATTATAATAAAAACTTCAATAACATTTTGTATCTT130.31295137217140107No Hit
CTGTAACATCGCTTTAGCATACAGAACAAGTCAACAAGATCTTAAATGTT120.2888781896966779No Hit
TACAAGCGACGAATTGTTCACATAAAATTAAAAACAAAATTACTTTTTTA120.2888781896966779No Hit
ATTTAATAAACTATGATACAATTAGAAATGTGTCCATAGGGCACGGCTGC120.2888781896966779No Hit
TGAAACTTTACAATGATATAACAATCTCAACTGAAACCTTACAATGAGAT110.26480500722195477No Hit
GTTTCCGAGGACAACATTTTAGATTTTGATTTTCCGTACAACATTTTAAG110.26480500722195477No Hit
GGAAACTGGTGTAAGGTCGGGAATACCATACGTTGTTGTTACATTGTACC110.26480500722195477No Hit
AAACTATATTATAAAAGAAATATGCATCAGGATAATCAGAATACCGCCGC110.26480500722195477No Hit
CTTCATGTCCTCCATCCAGAAGCCAACACATGTCAGGATATTGTCAGCCT110.26480500722195477No Hit
CAAAACTTATTTGACAGTTTTATATATATATATATATCTAGATATGGCAT110.26480500722195477No Hit
GCATTCTCAGGGATTATGTGTGTATTTGTTTGTATATAAAACATACCAAT110.26480500722195477No Hit
AAAACCTTTATGTGAGAAACTTAGTGTATACTAATTCATTTTAGCTTGAC110.26480500722195477No Hit
GTGGGAACTTGATCTTGGAATCATGGAACTGTTTGATGTTGGGTCGTCTG110.26480500722195477No Hit
TTTTATACAAATCATCATTTATTTATGATATGAAACAAATACATGTTGAA100.2407318247472316No Hit
CCTGATTCCTCAAAACGTAAATTAATGTTGTTTTCTACTACCTCTTGTTC100.2407318247472316No Hit
GCGAATGCTAACTGGACTATTCTTCCACTGATGTCCAACCCATCTTTCTC100.2407318247472316No Hit
CACATCGTCACGGTCGAAGTAGTAGGCCATAGACAAGTACACGTAGCTGG100.2407318247472316No Hit
ATAAACTCCCTAAATTTTCCTACTATTATTTCCATCCCTCATCCTCTAAT100.2407318247472316No Hit
TGACAGTGCAGCTACTAATACGAATGCTACTAATAATGAGGAGACTATTA100.2407318247472316No Hit
CTGCATTGCATGGGGTGGATCTCGTTCTGGCCAGCGGTGGCTATTGTCTG100.2407318247472316No Hit
GTACAACATACAGCAGGCCATGTACTTGCCGTGACGTGGGTCACATTTCA100.2407318247472316No Hit
CGAATATGTAGCATTTGAGAAAGTATTTTCACGAGAAAGTGTTCCTGTAG100.2407318247472316No Hit
TTAACTTTGATCCACAAAATGTCACAATGGTTGCAATAACGTCAGTAAAT90.21665864227250844No Hit
GAGCCGATTACCAAACGGAGCCTCCACCTTTTCAGCTGAACTCAAAGCCA90.21665864227250844No Hit
TAATAATATTGTAATAGCCCCCGCCAACACAGGCATAGCAAGCACCAGCA90.21665864227250844No Hit
GGCCATTCTTATAACAACCAAAGGTTAGGTAAGAAAATTCTCCATTGTCG90.21665864227250844No Hit
CTTGCTTTCCTTTATATTCGTAGCACTGTTCTGTATCGATTCCATGGTTA90.21665864227250844No Hit
CTTAAGTTTACTCATACCCTCTATTTTCCTTTGAGAGAAAATGATGCCAA90.21665864227250844No Hit
CAAACTCGTCCTTTTCGGCAAGTTGGTTAGCATCCAACCAGCTAATAACT80.19258545979778527No Hit
AGATATGATGTGGTTGATATTAGCCAATGGGATGTACTCTGAATAAATAC80.19258545979778527No Hit
GGAAAACGTGTATCTATGGAGTAAAGGGTTAAACAGGTCAAACTGATGCA80.19258545979778527No Hit
GAAAAAGTCGCCAAGCACGGTAATCACATGTAAATGCCGCAAACTGAAAA80.19258545979778527No Hit
CAACTGGTTACATAAGATTTGAAAAGTTTTTACCAATGATGACAAAAGTT80.19258545979778527No Hit
CAAGAACTTTTTTTTTGTTCTCACAAAACTCAATTAACAACAATATAAAT80.19258545979778527No Hit
CTAAAATCTCATCATGCTTTTGCAGATAATTCGCGACATTTTACAAGGTG70.16851227732306212No Hit
GAACTTACAGGTTGCTTTATATATATATATATATATATTTCATTAAGAAT70.16851227732306212No Hit
AACAAACTTGTATCTATTATATTATTTATTACATTATTTACATATATATA70.16851227732306212No Hit
TGTTCCCCTAGATAGTCCTCTGTTGATCCAATAATACAACAGATAACGTG70.16851227732306212No Hit
TTGAACTGTAATTCAGCATCTTATTTGTAATAACCATCTCTCTTCAATTC60.14443909484833894No Hit
GCTTAACTGTCTAGCTATGTTCGGATATTTTATGTGAAAACATTTCAAGT60.14443909484833894No Hit
GCCACCTGCATCACGTCATGTACAATGTTCTCATACTTCGCTGGCACACA60.14443909484833894No Hit
CTGAAATCAATTGCATTAATCATCAAATCCCGGATTACTGCTGCCGCATC60.14443909484833894No Hit
GGGAACTGTTCTTCGTAGAATTTCCAGAATTTGGACTATTTTTATTCCTA60.14443909484833894No Hit
CTTTAATGTTTGTTTGCTGTGCTCCGACCCAGTATTTTCCATGTGGGCAG50.1203659123736158No Hit
TACACGCGACGAATTGTTCACATAAAATTAAAAACAAAATTACTTTTTTA50.1203659123736158No Hit
TTTTAATATAACTTTAATCACAAACATTGTATACCACATTTGGCCACAAT50.1203659123736158No Hit
GATACATTCAATATTTAAACTAATCACATTTTCACACAGCCTTTATTTAC50.1203659123736158No Hit
CAAGAACCTTTTTTTTGTTCTCACAAAACTCAATTAACAACAATATAAAT50.1203659123736158No Hit
GCGGGAACTTGATCTTGGAATCATGGAACTGTTTGATGTTGGGTCGTCTG50.1203659123736158No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)