FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-ctenidia-RNA-7_S51_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-ctenidia-RNA-7_S51_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1258
Sequences flagged as poor quality0
Sequence length35-151
%GC40

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC100.7949125596184419No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC80.6359300476947536TruSeq Adapter, Index 12 (100% over 50bp)
AAAAAAATGAGACAAAAAAGAAAACTTCCGACAAAATAAATAAAGCCATC50.39745627980922094No Hit
ATGTTATCATGTTACATGTATGCGTACATGGGAATGCAACTATACTATCT40.3179650238473768No Hit
TTAACATGTATTGGTATTTATAAATGTTCAAGGGGTTAGTAGGTTCAACG40.3179650238473768No Hit
GATTCATGTAAGTCCGAACTAACCCAACTAGTAAAAATAGAGTGCCAATT40.3179650238473768No Hit
GTGGTGGTTGAAGAACTGTTGCAGTCTCTCATTGGTGTAGTTGATACACA40.3179650238473768No Hit
AACAGTAAACACATCTTGGACAATTAAACACTAGGTATTGTAGAACAATT40.3179650238473768No Hit
ACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC40.3179650238473768No Hit
AACTGAAATACACATAATTGGACTATGACCTATCGTTGTTCATACAAAAC40.3179650238473768No Hit
AATTTTGCTGTTCTTGCTGCGGGTGATTTGATGAGATGAATATTTTTCTC40.3179650238473768No Hit
GGCGACGCAGAAAAGGCCGGAGTAGGTGTAGATAAAGCCACTAAAGTATC40.3179650238473768No Hit
CAAAAATCTATAATTATTTAAAATCAACATGATATATAAAATTCATTATT30.23847376788553257No Hit
CTTACAGCAGCTGCAGCAAAGTATTAATGTATAAGCCAGGCCCGATTAGC30.23847376788553257No Hit
CGACGTTTTACGCAACTCCGATTTTTGAAGCTCATTTGAAACTTTTTCTG30.23847376788553257No Hit
CTCGACAGGGTCTTCTCGTCTTCTAAAATTATTTAAGACTTTTCACTTAA30.23847376788553257No Hit
TGTGCGTGTGTGTTTGTTTGTTTGTGTGTATACATGTGTGTGCATGTGTG30.23847376788553257No Hit
CACACCAATCCCATCGCAGCCCCTGACGGAAGACCGGAAAATACAAAAGA30.23847376788553257No Hit
GTATCCAAGTGTAGCGGAAAAGGAAATTGAAGTAAATCACTTTGCATCGG30.23847376788553257No Hit
TTGGGGTCCATCTGGAGAGCGGTCAAGATTTTGTCGGTGACCTGCTTGCC30.23847376788553257No Hit
GAAAGAGCTTGTCGCGTTCTGCAACGTTCCATGGTGGAATAGGTCTCCAC30.23847376788553257No Hit
AGAGGTGTATTCATGGGGTCGGCGGGGTTAAAATTCTCTGGGTCCATCAG30.23847376788553257No Hit
TGGAGATGGGCTGGCCCATATCTACACATACATTACCACGTACAAGACCT30.23847376788553257No Hit
CGAGGATGGAGTATCTCTGTTTGAATTCAGAGTAGATGATCCTGTTGGGG30.23847376788553257No Hit
ATTCAGAATGACTTTGGATTTGCTCTTCTCCAGGGAGATATTGTTGCCCA30.23847376788553257No Hit
CTCACCTTCTAGTTGCTGCCCTTTACACTTCCTTCTCTTCGAACTACTTA30.23847376788553257No Hit
ACTCGACCTCATCTTGCAAGTTTAATTCTGTACTTGTGTCTAATATCTCG30.23847376788553257No Hit
ATTGGAATCTGCAGGGTTTAGGGTCTGGATTGGAATCTGCAGGGTTTAGG30.23847376788553257No Hit
GTTTTGTTTTTACTTTTATATCTATCAACTAATCAACATGAGAAACTATC30.23847376788553257No Hit
GCCAACTGCAGATTTGTGTTATTTCTTATTTCCTGTAACCTGTTACCCTC30.23847376788553257No Hit
GTCATCTTTCTTGAAGGTTTTAATATCGTTATTCTTCTCGATCTTGACTG30.23847376788553257No Hit
CTTGGACACACCAACAGTTTTACCAATACGTCCAGTGGTCTTGGTGTGCT30.23847376788553257No Hit
GCGCGATCCAAACATATTTTTACATCTTAGATTACATACAACTTACAGTT30.23847376788553257No Hit
CAAGATTATTATGTAAGAAACAGCAAAAAATTAGAAAATAAACGAATCTC30.23847376788553257No Hit
GACGACCACCGCCATATGGTGAGGTGAGGGCAAAGTCAATGTGTTTCTGC30.23847376788553257No Hit
CGTCATTCCTACTCTCATATACAAAACATGAATGTAAATATATGTATGTG30.23847376788553257No Hit
GTTCAAAGTCGCTTTGGATTTGCTTCAATACTTTTGAAAATATGTTCCAG30.23847376788553257No Hit
GTGGAATCCTGCCGGCCATACCTGACCACGCCATTCTGATGTCGTTCTTA30.23847376788553257No Hit
GTTCACTGAATGACGTCTCTCATAAACATATATTTAAACATAAAAAAAAC30.23847376788553257No Hit
GCTCTACTGACTCATAGTGGCCATAGAACTTTTTGAAATTCTACTATGCA30.23847376788553257No Hit
CTTCATGTGACCAGAGTTTATAAATCCATGAGCCTCAAAATTAATTTAGA30.23847376788553257No Hit
TTTTTTGCACCCGGCCTCATCCTTTGTGATTTGTTTGCACGTGATGGGCT30.23847376788553257No Hit
CCCGTGGTTCAAATTGGTATATAAATATAAACAATATCCCCCTGTAATAA30.23847376788553257No Hit
TTTTAAAAAAAATCTCGTATGAGATTGAGTATTGGAAGTTTAGTGAACTC30.23847376788553257No Hit
CAATTCTGTTCTTTTTACATTTTCTATATCAGATAAATTCTGGTTAATAC30.23847376788553257No Hit
AAAAAAGCAGCTATTATTCTCAATAAAAAAATAGTAGTACCAACCCACCA30.23847376788553257No Hit
GTACCCTCTCTTGTATATCAACTCGCGAACACTCTTCAAGTTTGGATATC30.23847376788553257No Hit
GGAGATGCCAGTTTCCTATCCTTCCATACATGTCCTTGACTAAATGAATG30.23847376788553257No Hit
GTTTTTCTCTTCTTCTTTTTGCAAAAGTGGTATGTCAATCATTATACCAG30.23847376788553257No Hit
TGCAAACCGGCCGCTTAAAACTCTTATCCCATTCAATCTAATTGTAGCAT30.23847376788553257No Hit
CGGAAGAAAACCTTCACTGAGTCGTAGATGAACCACTGTAGGGCTGTCAG30.23847376788553257No Hit
GCAGAGAACAACAAACATATTATTACAGCTGTATTCATCCATTTCCATCA30.23847376788553257No Hit
CTAGGAAGTAAATGAGACTGTTTGAACTTTGCCATTTGACAATCACAAAT30.23847376788553257No Hit
GCTTTTCAAAAATGGAGTCATATTTTTTTTATAATCAAGGTGGAAGTTTT20.1589825119236884No Hit
AAACTGTCTGCCTGGACAGAGAGTCATATAGGAATGGCTAACGTACAATA20.1589825119236884No Hit
AGGGATAGTTGTGTTACGTTTGATGAGGGATGTCATCACACCACCAGCAG20.1589825119236884No Hit
TGTAAAACCTGGATGAGAACTGGCATCGTCACCTTGACCTTGACATAATC20.1589825119236884No Hit
AGAGGGCTGAGTTAAATCGTGAACTCGAAGAGCTCGGTGAACGTCTTGAT20.1589825119236884No Hit
TTTCAAGATACACTTTTATTATACAATGGATAAAAAAAGGTTTCATTCAA20.1589825119236884No Hit
CTTTAGTTTCTCAAACTCACCCTCAACCTTACTTAAAAGTAAGCAGTAAA20.1589825119236884No Hit
ATAAAAGCAAATTCAGTTTATAGCAATAATAAGTCTGCTCAACAATTTAA20.1589825119236884No Hit
CAATTTAAACGATGTCAATTTAAAGGTTCAACAACTTCTCCATCATGACC20.1589825119236884No Hit
GAGAGGCATCGGGTTGACCGTGGGATCAAACACCCCACCTCTGGTGTGAG20.1589825119236884No Hit
CTTATATCAAACAAATATACACTATACCACTTTAAAGAACGAATTAGTAT20.1589825119236884No Hit
GTGATATCTCCTTCCAACTTTCTCTTTTGTCCGCCAAGAGATTGCACTTG20.1589825119236884No Hit
ACAAAGATAACTAAAAATTAGAGATAGAAACCGACCTAGTTTACACCGGT20.1589825119236884No Hit
AATTAATCTATATTTAGAACAAAGGGAAAGGGATATACAGTAACTGGATA20.1589825119236884No Hit
GGCAACCTTGTGCAGGTCCAGGAGGGCCTGGTTGACAGTCTTCTCGAGGG20.1589825119236884No Hit
TGAGATTTTGTATTCAATTCTCAAATAAAACATAATTCAATTTAGAAGTA20.1589825119236884No Hit
CAAAGATCTGCATACCTCCTCTCAATCGAAGTACAAGATGGAGGGTGGAT20.1589825119236884No Hit
GCTTCTTCCAGTTTGGTATTCGTTCTTTCCAAGTCATCTTCTATGAGTTG20.1589825119236884No Hit
GCCATATAGGAAATAAAACAGATAAGAAAAAAATAACCCCCCAAACAATA20.1589825119236884No Hit
TTTTTTTTAGTTTTTTTCTCTTTATTTTTTTATAAGCAGATCACATTTTA20.1589825119236884No Hit
TTAATAAACGCTCAATTTCTTGCACCATCCATTATCAGTTATAGCACCTT20.1589825119236884No Hit
CTTGGATCTTGGCTTTCACATTCTCAATTGAGTCGGATGCCTCAACCTCA20.1589825119236884No Hit
CTCTGCTTAATATTCAGTAGACTTCTGTGCAGAAATATTATATTAATAAA20.1589825119236884No Hit
CTTTACTCATGTGGACTATGAGGTCCACTACACGGCAACTGTAACCAAAC20.1589825119236884No Hit
CTCAACACACATGGGCTTTTGTGGAGAAAGGACAACCATAGCGGCATCGC20.1589825119236884No Hit
GTATATCTTGTCTTCTACCCAGTTTGAAAACTTGTCGAGACATCCTTCAC20.1589825119236884No Hit
AGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTC20.1589825119236884TruSeq Adapter, Index 12 (100% over 50bp)
GTAGACATTGGACTGGCCGTCGGTAATGATAACGATTACATTAGGAACGT20.1589825119236884No Hit
CAAAAGTACAAAGCGATCAGCCCTTTTCAACTTTAAGGACGATACCTAGT20.1589825119236884No Hit
AGCATCCTCCTCATCATTTTCATCAATTTTATTTTCAAAGTCTGGAGTAC20.1589825119236884No Hit
AGCAATTCAACTAAATACTTTAATTCCTGTAGGGATAGCAATAATCATAG20.1589825119236884No Hit
CTTGTCTTTGCCTCGCAATTTCACTCCAAGCACACGCTCCAGTTTTCCCA20.1589825119236884No Hit
AAAATTACATCTAAAGAAGCACTAGAAAGAACAATCCCAGTTAAGCCCCC20.1589825119236884No Hit
GGCTGGAACACCACAAATATCTCGAACTACTTATCAGGTCCCGGCCGAAT20.1589825119236884No Hit
GTCAATGCACGTTTATCAGGCACATGACGTTATGTACAATTATATTTTTT20.1589825119236884No Hit
CCAACTTAATAGTCCTCGGTTTGTCTATTGTATCGTCGTCAAAAATGTCC20.1589825119236884No Hit
ACACTTATTACGTATAACTGCATGCTGAACACTCAACGCTCTGTGTACAT20.1589825119236884No Hit
GAAATATCGATATTGTCATGTTTTGGTGGAATTGATATACCAAATAATTT20.1589825119236884No Hit
ACGGGGCTCGCTCCATGGGCTCAGTAACTACCCCTTTGGAATTCTCGGGT20.1589825119236884No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCACCCCCCCCCCCCCCCCCCCCCCCC20.1589825119236884No Hit
TACGTTTACAGAAAATCATTGAACTGAGAGAGTATAAATTACAACAAGAA20.1589825119236884No Hit
CTCGCGAGTAAAGTGATTTGATAATTGTAATCATTTTACAGCTTACACCG20.1589825119236884No Hit
CCGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC20.1589825119236884No Hit
AGATATTATAAACTAACATAGACATTATGAATTAACTTAGACATTGTGCA20.1589825119236884No Hit
GTTAGAGCTCATGTATTTATTTTTATTGTCAATAGAATAAAATGTAGTAA20.1589825119236884No Hit
TAGATTTGAACCATGACAAGCTGTGTACCACCAAGCGCCAACATGTGAAG20.1589825119236884No Hit
GTAATTTTGTAAATAATATGAATACGAAATACAGTTGAAGTATGGGTGTG20.1589825119236884No Hit
GCTGCCTTATGAGAGAACACAGCAGACAACACAACTGTAATCTAGCATAA20.1589825119236884No Hit
GTCTCCAAGTGTAGCGGAAAAGGAAATTGAAGTAAATCACTTTGCATCGG20.1589825119236884No Hit
CGTACATACCTTTTTCACATCCTTTAGGATAAATAAATAAATATGTGTGA20.1589825119236884No Hit
TTAACATCGTTCAATTCGTCATCCTTTTGTCGGTACTGCATTCTTAATTC20.1589825119236884No Hit
CAGTATTTCTTTATTTGAACAATATATCGAAATAAAAACATTAATCAAGA20.1589825119236884No Hit
GCAATACAATATATACATATAGCATGTTGTTTTTTTTGATAAGTGTATAG20.1589825119236884No Hit
AATACATCAATGTTGTGCAAGGGCAACAAGAACCATAACTCTGCATGCCA20.1589825119236884No Hit
GTAAATCTATCACAATATTATATGCAATTGTACTAATATTCTCATTATGC20.1589825119236884No Hit
AGTGCACCGAAAAGATAACAAACACATGCTCCCCAAATGGAGATTACATG20.1589825119236884No Hit
CTTTATGTAACATGTAGTTCAAGTGAGTGTACACACCGGAATGAGAATTA20.1589825119236884No Hit
AAAAGTGACTCTGGGCAACGGAATCTCTCGTTACCGATGGTGATGACTTG20.1589825119236884No Hit
GCAACACGGATGCCCATTTCGATGAAAAATAAGACAAGAAAAAATTGGAA20.1589825119236884No Hit
CTGGAAACGTCCGTGACCAAACTTGCTGGCGGTATCGATGAACTTGAGGG20.1589825119236884No Hit
AGATATTCAACATGTTGAGGAGAGTGGCTTCAGAGGCACCCACCTTTGTT20.1589825119236884No Hit
CTTTCAATGTTAGAGGTGTCAAAATGCGTCTATATGAAGTAAAGGGTTAA20.1589825119236884No Hit
CTACCATGTTGTCCCCATGCCAACCAGAAATTGGTACAAATGGAACAGCT20.1589825119236884No Hit
CGTTACCGATGGTGATGACTTGACCGTCGGGGAGTTCGTAGCTCTTCTCA20.1589825119236884No Hit
CAACGGTATACATTGCTGTAGTGTCACTATAATCTGCACCATAAAAGAAA20.1589825119236884No Hit
AATCCAACACTGGGGCATATCCATTTTTGATCTCTCCAGGATGGTTAAGA20.1589825119236884No Hit
GTTACGTGTCATCATAAAAATTTGGAACTAGGAGGGAAAATCGAGTGGCC20.1589825119236884No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20.1589825119236884No Hit
CCGGCATCCGTTGCTCCAGGAATATGCACCATCATAGGCGCATGTGTAGC20.1589825119236884No Hit
CACTTCACAGACTACTACTGACCTTAAAAATATTAAAGGGTCAATAAAGT20.1589825119236884No Hit
TCAGATTTGATCAACTTCATCATCATGTGGAACATTTTGTCGGTTGTTTG20.1589825119236884No Hit
GTTAAACTATGTATATAAACATTCTTCATTTAATACTACCAGGTTTAAGG20.1589825119236884No Hit
ACCATCTACACAGATCTTTCATAACTTACAATTTACACCTATGGAAAGAA20.1589825119236884No Hit
TGACAAAAAAAAACAACAATGTTTGATAGTTAAATGACAACAATGGGAAA20.1589825119236884No Hit
GTTTCTGTTAGTGTTGCTGGGATCTGACCTAGGAGGCTTAATGATCACCC20.1589825119236884No Hit
TACAAATATAAATGTCCACAGTTGGTTAGAATGTTCAGCGAATGGTCCTG20.1589825119236884No Hit
TCACTGCTTACGTTTTCATATTATAATACATTCAAGAAATTGTGAGTGAC20.1589825119236884No Hit
TTTTTTTATTTCCAGCCTAAATACAAAACATTTATTTTTTGCTTGCTTCT20.1589825119236884No Hit
CTTCGAACTACTTAATTATATTTCCTCGCCCAAAACACCTCTCATCAGCA20.1589825119236884No Hit
CCGAAAGGTCTTCCTCCATCTTGGCCTTTGCTTCTTCTACATCCCTTAAT20.1589825119236884No Hit
GTCGTTCTTACTCCCCCTACATATGATAGTCTCCGCTGCCTTAACAACCT20.1589825119236884No Hit
GTTTCTTGCAGGTTATAGTGCATCAAAACAAATAAATTTATGCTGTATGT20.1589825119236884No Hit
AGAGACCAAAATTTTTTTTTTAATATTACCAGTAGGTATATCAATCGCCA20.1589825119236884No Hit
GTTTGATCGAGTTCATCACTGATCTGCTTGTACTTCTCCTTCTCTGTAAG20.1589825119236884No Hit
CCCAGAACTGCTAGTACTACAGAAACTCATGGACTCTTTTCTTGTATTCA20.1589825119236884No Hit
TCATCCCGGCGAGCTAAAAATAGTTCTTCTGTAGGTGCTGCTTTCTTCAC20.1589825119236884No Hit
AGCAGATTCCATTCCCAGGAAGGATGGCTGGAAAAGTGACTCTGGGCAAC20.1589825119236884No Hit
GTTGTTGTTGCTTATTGTTGTTGCAGTTGTTGTTGGTGTTGTTGCTATTG20.1589825119236884No Hit
CAACATTTAACCTCATAAAGAAAAAATAACAAAAACAAAATTTACAGGTT20.1589825119236884No Hit
GACTGAAACAACTTTTCCTGCGAGTCCTTTGCAACGCAGTTGTGTTAAAC20.1589825119236884No Hit
GGTAAATGTCTGTGTTTGCTTGGTAGGGATAGTTGTGTTACGTTTGATGA20.1589825119236884No Hit
CTTGTTAAAAAAAGTGGCTTGAGCAAATACTATGAACACTGTGGAAACAA20.1589825119236884No Hit
CGGAAACCTTGCTATCCGTCCACCCATCAAGTACACACCATTATGTCTTT20.1589825119236884No Hit
GTTAAGTGGAAAACGCAAACCCTCCTAGCCTGCACAGATAAAATCAACAT20.1589825119236884No Hit
CAAATGTTTGATCGAGTTCATCACTGATCTGCTTGTACTTCTCCTTCTCT20.1589825119236884No Hit
GGAAAACACATATCTATGGAGTAAAGGGATAGCACACAAAGGATTAATTT20.1589825119236884No Hit
ATACTTTTGTAGAAAGTCTTTTCTGCTTCCTAGTTGCCCCAACTAAACTT20.1589825119236884No Hit
TCGCTCGTTACCAATGGTGATGACCTGACCGTCGGGAAGTTCGTAACTCT20.1589825119236884No Hit
CCGTATTTCTTTATTTGAACAATATATCGAAATAAAAACATTAATCAAGA20.1589825119236884No Hit
AAACATATTCATGTCTGGAAATTCCGTACTTTTTTTCATAAAAACATTTC20.1589825119236884No Hit
GTTGATTCAGACATTACTTAAAAGTAAAAATAATGTAGTGTTATACTAGT20.1589825119236884No Hit
TTTATTCTTTTAATTTACATACAAGTCACTTCCTTGGTTGGTGTTTAAGC20.1589825119236884No Hit
TTTGGTGGACATGAATCATAATGATATACCAAACCATTCAGATTTTTTTT20.1589825119236884No Hit
AAAAAATTTAAAAGGGCAATAACTCTGTAATTAACAATAATAGTGTTTCT20.1589825119236884No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)