FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-ctenidia-RNA-4_S27_L004_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-ctenidia-RNA-4_S27_L004_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3609
Sequences flagged as poor quality0
Sequence length35-151
%GC37

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2807.758381823219729Illumina Single End PCR Primer 1 (100% over 50bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT421.1637572734829593Illumina Single End PCR Primer 1 (100% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN90.24937655860349126No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.1662510390689942No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC60.1662510390689942No Hit
TTTAGAATATTTAGAATAAAACATCATCCACAACCAATCATGTCAAGCCG60.1662510390689942No Hit
GACCTATATAAGGAATTAAGTGTGGTTCCATATTCCACATGTCGTGTCAA50.13854253255749516No Hit
CTTGTACGTGTAAAATAATTTTGACCAAGAAAATACTGTCAGTAGTTATG40.11083402604599613No Hit
TGAAAGTAACGCCAATGATAATTGAATTTACATTGTGGTCTCCGTATATC40.11083402604599613No Hit
CGGCAAGAAGGTCTGTTCGGACGTGGCACATTATCTTAAAGACAGGGCGA40.11083402604599613No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)