FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-ctenidia-RNA-4_S27_L004_R2_001.fastp-trim.20220908.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-ctenidia-RNA-4_S27_L004_R2_001.fastp-trim.20220908.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3145
Sequences flagged as poor quality0
Sequence length41-151
%GC36

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1585.023847376788553Illumina Single End PCR Primer 1 (100% over 50bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT270.8585055643879174Illumina Single End PCR Primer 1 (100% over 50bp)
TTTAGAATATTTAGAATAAAACATCATCCACAACCAATCATGTCAAGCCG60.19077901430842606No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC60.19077901430842606No Hit
GACCTATATAAGGAATTAAGTGTGGTTCCATATTCCACATGTCGTGTCAA50.1589825119236884No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.1271860095389507No Hit
CGGCAAGAAGGTCTGTTCGGACGTGGCACATTATCTTAAAGACAGGGCGA40.1271860095389507No Hit
CTTGTACGTGTAAAATAATTTTGACCAAGAAAATACTGTCAGTAGTTATG40.1271860095389507No Hit
TGAAAGTAACGCCAATGATAATTGAATTTACATTGTGGTCTCCGTATATC40.1271860095389507No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)