FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-ctenidia-RNA-4_S27_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-ctenidia-RNA-4_S27_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3609
Sequences flagged as poor quality0
Sequence length35-151
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC2526.982543640897755TruSeq Adapter, Index 12 (100% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC421.1637572734829593TruSeq Adapter, Index 12 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90.24937655860349126No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC90.24937655860349126No Hit
GCTCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC90.24937655860349126TruSeq Adapter, Index 12 (98% over 50bp)
ACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC80.22166805209199225No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN70.19395954558049322No Hit
GGGGCACATGTCATCATCTCATCCAGGTCCTGGTCTGTGGGGCTCTTACC60.1662510390689942No Hit
ATCAAAATACAAAATAAATTTGGGCTGCCAGGGTTACAGGAATAGTCTTA50.13854253255749516No Hit
TTCTCTGTTTGTTGTTTAAGAAATCTCCAACCGTCTCCTAAAATTCCAGT40.11083402604599613No Hit
ATCTGACCGACAGACACAGACGGCAGACCGACGATCCGACTAACTGACCG40.11083402604599613No Hit
TAAGGTTTCCCTCCATCAATAAAACTGGATATTCCCTATATAACCTGATA40.11083402604599613No Hit
NATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC40.11083402604599613TruSeq Adapter, Index 12 (98% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)