FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-ctenidia-RNA-4_S27_L004_R1_001.fastp-trim.20220908.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-ctenidia-RNA-4_S27_L004_R1_001.fastp-trim.20220908.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3145
Sequences flagged as poor quality0
Sequence length41-151
%GC36

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC1484.705882352941177TruSeq Adapter, Index 12 (100% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC280.890302066772655TruSeq Adapter, Index 12 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.22257551669316375No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC70.22257551669316375No Hit
GGGGCACATGTCATCATCTCATCCAGGTCCTGGTCTGTGGGGCTCTTACC60.19077901430842606No Hit
ACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC50.1589825119236884No Hit
ATCAAAATACAAAATAAATTTGGGCTGCCAGGGTTACAGGAATAGTCTTA50.1589825119236884No Hit
TTCTCTGTTTGTTGTTTAAGAAATCTCCAACCGTCTCCTAAAATTCCAGT40.1271860095389507No Hit
TAAGGTTTCCCTCCATCAATAAAACTGGATATTCCCTATATAACCTGATA40.1271860095389507No Hit
ATCTGACCGACAGACACAGACGGCAGACCGACGATCCGACTAACTGACCG40.1271860095389507No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)