FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-ctenidia-RNA-3_S19_L003_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-ctenidia-RNA-3_S19_L003_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1390
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG664.748201438848921Illumina Single End PCR Primer 1 (100% over 50bp)
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC624.460431654676259No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT110.7913669064748201Illumina Single End PCR Primer 1 (100% over 50bp)
TGTATTTTTTGTGATGACAGGATTTCATGGGTTGCATGTTTTAATTGGCA60.4316546762589928No Hit
CTTTAGCTGATTGAGCAAAACAAATTCCAAAATGGGTAAGGAAAAGACTC50.3597122302158274No Hit
GCGAATTTGACGAAGAAGAGCAAGAAGAGGAACAATGATAGAGCAACAAC50.3597122302158274No Hit
AACTCATCATTGGTGACAGACAGACTGGTAAAACTGCCATTGCTATTGAT50.3597122302158274No Hit
TGACCGTAGCCGTTGCTGTGGTGGTCTACGTCACGTATGTGAGCCACATA50.3597122302158274No Hit
CTCGAGGTTGAAGCCTCTGACTCTATTGAGAATGTGAAAGCCAAGATCCAGGACAAAGAAG50.3597122302158274No Hit
CTTGTTAAGTTTTTAACTCACATGAGCCAAAGGCTCTTAGGTAAACTTGT40.28776978417266186No Hit
AGAGAAGGGAACTAAACATGTTTATACAGGTGTGCTGTTCACTGGTAGTT40.28776978417266186No Hit
GGGAGATACATCAAAGTGTTCAATACAAAACATCAACAGACTCCGATCAC40.28776978417266186No Hit
TTTTGTTAAGAATTTAATAATTGTCTTGCACATAATTTGACATATGTTTA40.28776978417266186No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.28776978417266186No Hit
GTGATTTGTTATAAAACATGACTGCAACAGTGAGGTGCTAGTTTACTATA40.28776978417266186No Hit
CTTACTCAGAGGGAATCCGGGTGCACTATATATAGAACTGTCACTAGTAA40.28776978417266186No Hit
AACCGCCATTATGCGACTACCCCACGGAAGCACTTCCTTTTTGCAATGGT40.28776978417266186No Hit
TAATGAGATAGGATGACAGTTTACTGAATATAAATGATAGCCTAGGGTTA40.28776978417266186No Hit
GAAAGATTGACTTCAAGAGGTTTGGTTATGTAGATGTTGCACCAGGCGAT40.28776978417266186No Hit
ATCACTAACCTTTTATGGCATCAAAGACCTTATGGAAGAAGAGAAATGTT40.28776978417266186No Hit
CGAAAATAAAACAGATTCAAGACGCTACCCGTAATGACAATCAATTACAG40.28776978417266186No Hit
TGAACACAAAATACGCAATGCTTGCTTACAGTGCACATTAAGTTTCATAG30.2158273381294964No Hit
GTCGCAAGGTGCTTGAGAGTAGGAGCATAGCAGATGATGACAGGATCGAC30.2158273381294964No Hit
TCCATGCATAGTTATTTAACTGTAACATTTTAACACCATACAAGTAAGCT30.2158273381294964No Hit
CATTCCAATAGTTAACTCTCCACTATTTATCAAGAGTTATGCCCCATTCC30.2158273381294964No Hit
CAGAGGAACACCCCGTTCTACTTACCGAAGCCCCGCTCAACCCCAAGGCT30.2158273381294964No Hit
GTGGTTTAACATCATTTTGTGAAAAGATGCACAGTACTTCCACAAGTAAT30.2158273381294964No Hit
CAAACCATGGCATTGTAAAAACTCATTTAAGTATATAATGGAGTTCTGAT30.2158273381294964No Hit
GTCAAGCATAGTAAAATCATATGAAGCAATTGACATGATTCCCATATGAG30.2158273381294964No Hit
TGACCAGTTTGTAATAATAGTACTGTTCTTTTCTTTGATATACGGCCAAA30.2158273381294964No Hit
CTTTCTCCAATTTCACAATGTCTCCGAAATCAATAATGTAGGGCAATTTC30.2158273381294964No Hit
AACTGACCTAGGTCAAACTTTCCATTTAGCATTGTCTTATCAATTGACTG30.2158273381294964No Hit
CTCACATCAACATTGTTGTCATTGGACACGTAGACTCTGGCAAGTCTACC30.2158273381294964No Hit
TGTCTACTTCTTATATGTTTTCTATATATGAGAAAATATTTTCCCAGCGT30.2158273381294964No Hit
CCCAGACGCTAGTAGGGTGTCTGGGCTACATTTAGAGTGTGTGTTTACAT30.2158273381294964No Hit
TGGCAGAAATGTCAAAACTACAAAATACACTAACCAATCAAGCCAAGGAA30.2158273381294964No Hit
TGACAGCAGAGTCACAATGATGTCAGCACTCCAAGTAATAAGTCAGAATT30.2158273381294964No Hit
GTACTGTAGATATAATGAATACATTTACAAATGTACAACATGAAATATTG30.2158273381294964No Hit
CGTCCCCGTACGCTCCTTAATTTTCATGCCGCCCCGACTCTTTTTATTTT30.2158273381294964No Hit
CCTAGAGTTCTGGAGTTGATATACTGGCGCGATGTCAAGAAGACGGGTGT30.2158273381294964No Hit
GGGAGCAAGAGTTGCTAGAACAGCTGAGCATTCCACTAAACAAAAGTATA30.2158273381294964No Hit
AGTGCATGTAAAATTAGAGTAAAAAAATATATTAGAGTAAAATATTTTTA30.2158273381294964No Hit
TTCTGACTGGTTCCCTATTAAACAGGGTACCAGACAAGGTGGAGTCAGCA20.14388489208633093No Hit
GCGTGGTCCAGTGGTAGAGCTCCGGACCCATGGTCGGAGGGTTGTGGGTT20.14388489208633093No Hit
CAAAGGTCGATTTTCTGTGGAGTTGTTATCTGAGAGAAAACAAAACAGCT20.14388489208633093No Hit
CGTGTCTTTACTGTTCATACAAAAACACTCATTACAAAGGCGAGTGAAAC20.14388489208633093No Hit
TGTTTGGAGAAGTGGATCGTTAGTTGATGAACCTTATTTATTTGTGAAAA20.14388489208633093No Hit
ATATAACAGTTATCTCCCTTTGATCATCTAACGACCAGGTCTTACAAGGT20.14388489208633093No Hit
TGGATGTGCCTTTGTTAATATCGTGATTGTTTGTTTTTATTTTCGACAAC20.14388489208633093No Hit
TAAGGCATTCATAAATTTGTTCTGATACAAATTTTAATGATGCTTTCAAT20.14388489208633093No Hit
CCCCCCCCCCCCCCCCCCCCACCCCCCCCCCCCCCCCCCCCCCCCCCCCC20.14388489208633093No Hit
CCCTGACATTATTAAGCAGGGTATAAGGAAAGGGGAGCTTGGATTATTAC20.14388489208633093No Hit
TTTTAAGGTGACCATTACACTACAGAACTTATATTTATGAAGTTTCATAT20.14388489208633093No Hit
TGTGTAGTCAGCAGTAATCCAGGTGCCACTATGACAATTCAAAAAGACGG20.14388489208633093No Hit
CGTGTAATCTGGGGAAAAATTACCCGATCACATGGAAACAGTGGTGCTGT20.14388489208633093No Hit
TATAAATGTCACACTGGTATGGAAGACGCCACTCTTGTACTAAACATAAC20.14388489208633093No Hit
CGGCACCATCAGCCGTGAACACCCATGGGAGGTCATGGTGGATTTGTACT20.14388489208633093No Hit
TAGCCATTGAGATGGCACTTCAAGCACAAGCTATCCAATCAGAATCTCTG20.14388489208633093No Hit
CAGAAGTCCACCATTAATGAGGTTATCACTAAGGAATGTACGATCAATGT20.14388489208633093No Hit
GTGAGCCGTCGAGGAAGGCAGACAGCGACCTTTCCCGTCATACCAACAGG20.14388489208633093No Hit
TGTCTGTCGGATTTTATTAATAAATAAATATGTGTGACGACGAAGTTGCT20.14388489208633093No Hit
ATTTCATGAGGCTATTCACTTTACTTTTTTTATAAGGAGATATATTTTTG20.14388489208633093No Hit
CTGATCTTAACTCAGACAATGATATTGTGAGAATTCATGTGTTATTTATT20.14388489208633093No Hit
AACGAAACATCAATAGTGTAATAGGCCATACAAGTCTGAACGAAACAACA20.14388489208633093No Hit
GCGACTGGTGGTCGCATGAACCAAATGCGAAAGAAGAGGAAGTTTGAATT20.14388489208633093No Hit
CGCATTTGTCACGTGCTAACTTCGCTCTGACCAAACGAATGGTTGACGAA20.14388489208633093No Hit
AACATGTAGTGTTTGGAAATGTTGTTGACGGCATGGATGTTGTGAAGAAA20.14388489208633093No Hit
TTAATGTACAATATATGGAATAAAGAAATGTGATCATCTGTTTATCATGG20.14388489208633093No Hit
GATCGGAAGAGCGTCGTGTAGGAAAGAGTGTAGATCTCGGTGGTCGCCGT20.14388489208633093Illumina Single End PCR Primer 1 (100% over 30bp)
GGAATATTTACTACACATGTCATGCTTTAGACATTGCTGGAACTTGGTAT20.14388489208633093No Hit
GTTTGGTTTTAACAGTACTTGTTTCAAATTATCAGCATAACGACATGTCA20.14388489208633093No Hit
AGGAAGTTCAGCAGGATCTGCCCCAGCTGGTGGAGCCCCAGCCGCAGGTA20.14388489208633093No Hit
TTTTCATACATTTATTCTGATCAGAGACAAATAGAGATGAATAACTAGGT20.14388489208633093No Hit
GCGAGTGTGGTCAAAACCATTTACTAGTATGCCATACAGTGTATAAGCAA20.14388489208633093No Hit
TGAACTTGGATTTCAAAACAGTTTTATATCGAAGGAAAAATAACTGGAGA20.14388489208633093No Hit
CTGATCTTTGCAGAGGGGTAGGAATCCTCCGAATGGATGTCATGTTAATT20.14388489208633093No Hit
CGTTACTTTAACCTTTCAGTAAATATATAGATGTGTGCACACATTTTTGA20.14388489208633093No Hit
GTGAAATTATTCTTAAGGATTAACAAGAGCTCCCTCCCGTTTAGTTTTAT20.14388489208633093No Hit
GCCTCATGTCGCTGTTCGCAGCTCTTCTGGTCCTCGTCGTGTAAAGCGTC20.14388489208633093No Hit
TTACAAACTAACTTTGTTAAGTATATTAATTTACATCAATAGTTGATACA20.14388489208633093No Hit
GCAGATGGTTGTAATGGACAAAGCAGCAAGGCCAACAGATTTGCCAAAGT20.14388489208633093No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCACC20.14388489208633093No Hit
CCAGGTCAACTGAACGCTGATCTCCGAAAGATCGCCGTCAACATGGTTCC20.14388489208633093No Hit
AATAAGTTTCTCATGATAAAATAAAATTACTTTTTATCGGTTTGTGCGTG20.14388489208633093No Hit
CCTGCACAAGATTGCTGATACCCATGGTGATGCACAGATGTGTGATTTCA20.14388489208633093No Hit
TCAAGATAATTTTTTTTGCATACTAAACACATATTTATTTTAATTTAAAT20.14388489208633093No Hit
AGTGCCTTCCACGGGTGGGTTGTATGTGTAAATGGAATAGCCCTTAGTAA20.14388489208633093No Hit
TATCTAGTGCCAACATAATATTCCTTATCTAGTGCCACTATACTGTTATT20.14388489208633093No Hit
TGGAATTATGAACAAGCAGATGTTGTTGCCGTTGGTATTGGTCACAGCGC20.14388489208633093No Hit
GTCCAATTCAGATATGGTCACACAAACAATGTATATAACTACTGTATTAT20.14388489208633093No Hit
TTTAAAGGCATAAGTGGCCAGTAGTTCATTTTATTTGACCAGTTTTGATT20.14388489208633093No Hit
GCTTTTCTCTCCAAACCTCACCACAAATGGACTTGTTTGTTTGTGGAGGG20.14388489208633093No Hit
ACATTACATGGCGATCATAACCATTAAATATTGACTGCCTTAAAGTGCAT20.14388489208633093No Hit
TAACCAAGTAAAAAATATATGATTCTAGAAGTATTATATAAAAAAAATTA20.14388489208633093No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)