FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-ctenidia-RNA-3_S19_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-ctenidia-RNA-3_S19_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1390
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC755.39568345323741No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC614.388489208633094TruSeq Adapter, Index 12 (100% over 50bp)
ACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC130.935251798561151No Hit
CCCCCCGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC80.5755395683453237No Hit
GCCGCCTCCAACCCGAAATGATTATGGGCCTCCGCAAAGTGATTCATGTA70.5035971223021583No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC70.5035971223021583TruSeq Adapter, Index 12 (100% over 50bp)
CTCTCTCAGCCTTCAGTTTGTCCAAAACCCAAGCGTATTTGAAGGATCCT50.3597122302158274No Hit
GGAACTATCGCAGAGAAGATCTGGGTCGCGGCGACTTTGCATGCTGGTTT50.3597122302158274No Hit
ATCTGTCTGTAGGCAACGGCCTGTTTTGACAAATCATCATAGATGATGAG50.3597122302158274No Hit
TTTTAACTATTAAGTTTTGTTTATTTCGTCTTGATACATGGTTTCATTAC50.3597122302158274No Hit
TAAAAACTACACATTTTGATTGAGCATGTCAACATCCAATGAAAACTGTC40.28776978417266186No Hit
CGATATTATACAAATCATGTTTTGCTATTGTGCTGGGGAAGAAAATTAGT40.28776978417266186No Hit
CAAGATGTGTTAGTATGTCACGGTTGATCCAATGGTATTGCCGATTAAAT40.28776978417266186No Hit
CGAGGCTTTGGTTGTATTGTCTGCCTTCTATTGGAACTTTGTCGTGTTGG40.28776978417266186No Hit
GGTTCACCATGGTAAATGTCAGTGAAATTACATTGAAATCAGGTCAGTAG40.28776978417266186No Hit
AGTAAATTAACCTGTATTAAGCAACAACCTGTCTTAAAAGATCACCTTGG40.28776978417266186No Hit
AAAATACACAAGAAAATACAGTTTAAAACAAAGATTTCATACAACTAAAT40.28776978417266186No Hit
CTTCTTTGTCCTGGATCTTGGCTTTCACATTCTCAATAGAGTCAGAGGCT40.28776978417266186No Hit
CTTCTCCACCCGCCGCAAATGACGCCCCTTGGCAAATACCTGGGTTTCAA30.2158273381294964No Hit
GATCGGCAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC30.2158273381294964TruSeq Adapter, Index 12 (98% over 50bp)
CGGTTGTTCACAAATACGCATCATTATATACCAATATAGTTCGTATATAT30.2158273381294964No Hit
GTTACTTGCAAAAATGGGGTGTGCTTGAGTACTGGTTAACATTTCAGGAT30.2158273381294964No Hit
CCCGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC30.2158273381294964No Hit
CGAGCCCACACGGTTGATGTTGGTGATGTGGTCACTGATCTCCTTGATAG30.2158273381294964No Hit
AGCAACAGTTGATATTACAGGCTATCTTCTCAGCAGCGTTCTGTACAACG30.2158273381294964No Hit
GGATAGAACCACCAGGTTCCTCTGTGGCTAATTGGTCTGAGGCGCCATCT30.2158273381294964No Hit
TTTTGGCTTCAGCAGCTTCTAAACGAGTTTCGGCACGTTCAAGATCGACC30.2158273381294964No Hit
TAAATAAACACAGATTTATTACAAACAAGTACTACATTAAACAGTATCCA30.2158273381294964No Hit
TTTTGTTTAGAAACTGTATTTATAGTATTTTAAAAAATACACTTCAAGTA30.2158273381294964No Hit
ATCACCTCCTTGCACTCTACCACTGTCATCATCTCTTGCACTACCCCTTG30.2158273381294964No Hit
GAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAA30.2158273381294964RNA PCR Primer, Index 40 (96% over 30bp)
ACATGGCTGGGGTGTTGAAGGTCTCGAACATAATTTGGGTCATCTTTTCC30.2158273381294964No Hit
CTGTGCTTCCTTCTCGAACTTCTCAATGGTTCTCTTGTCAATGCCACCAC30.2158273381294964No Hit
CGATTTTCGTTTCCGAACCAATATTATACTAGATTCATAAGAAAACGACA30.2158273381294964No Hit
TTTTTCAGTTGTTATTGGTAGCAAAACCATGCACCTTATTTAATAAAATC30.2158273381294964No Hit
ATGGTGATCATTTTGGGTTGTGACGAAATGTTTGTCTGAATAAATGATCG30.2158273381294964No Hit
CTAATTTATCTGAAACTATTTAATTCTGTTACAGCAATAAACAGAAAACA30.2158273381294964No Hit
ATAATATTTACAGCAATTGCATAATACGCTCAGTAGAGACTTCATTCTGA30.2158273381294964No Hit
CCACTCTATGCTAAAACCAGTCCCTGCATGAATAAACAGCTATTCTATAT30.2158273381294964No Hit
ATTTTTTCTTTACATTCATCATATTAATTTACATTAAAAAATCTGTCATT20.14388489208633093No Hit
ATTACGTAGACCAAGGAAGCCTGCAATATGATTGGCTGCCATATACAATT20.14388489208633093No Hit
TTTTTTTTGTTTAAATATTTTTATTGGCAAGTATATACACATTTACATTT20.14388489208633093No Hit
TTTTGCAACCATATTTAGGCAGTTTATTTATTTTATGTTTACAGACAATT20.14388489208633093No Hit
GTCGCATTGACTTTGGTATAACCATTTTTTGTCCTTTAAAAACAATGTCG20.14388489208633093No Hit
TTATATTTCAAAACCTGTGTTATGTTTAGTACAAGAGTGGCATCTTCCAT20.14388489208633093No Hit
TGTGAATGAAATTGTTTTAAAAAGTTCTTGAACTTGAAACAAATAATATC20.14388489208633093No Hit
CGACATTGTGTCTCATTATCATTCGTCTCTCACCACTGATTCAAGTGGGA20.14388489208633093No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCACCCCC20.14388489208633093No Hit
GTTATCTCCTTAGTGTTATATTTAGCCAGCTGTTGCCGTCCGTCTTTTTG20.14388489208633093No Hit
CAGCGTTTCAGCAAATTCCATCATTTTACTCAAATTGTCGCTTCTTACGA20.14388489208633093No Hit
TATTACTATTTTGTTTTTATTTTTAATTTTTTTGTTTGTTTTTTTTTAAT20.14388489208633093No Hit
TGATACTACATGGTAAGTTTGACCACGGTCACTTGAGAAGACAATGGAGC20.14388489208633093No Hit
TTCCTGGGCGGCCAATACAAAGCATATGCGGAAAAGTGTCTTGCTCAAGG20.14388489208633093No Hit
CCGAAATTTAGTAGATAATACATCTATAAAAAGACAGAATTATTTATAAA20.14388489208633093No Hit
GTCGCCTTGACTTTGGTATAACCATTTTTTGTCCTTTAAAAACAATGTCG20.14388489208633093No Hit
CGGAGGATCAAAACTGTATCTATAAATGATTCGTAATATAGTTGTTTTAC20.14388489208633093No Hit
CCAAAACCATTTGCAGATTTTCTGTGGTGAATTTTTCCAAGAAAGCACAG20.14388489208633093No Hit
CGAAAACACATGACAATATTATAAAACATTACACGAGTATTTATTAAACT20.14388489208633093No Hit
GGGAATGCTCTATCACTAGACTAACATTGCGATTTATATTATGTTGATGC20.14388489208633093No Hit
GCGATTTCAACATTGCCATGTGTCCCTTTAAAACAACAACATTCCGTAGC20.14388489208633093No Hit
TATTACACTATTGATGTTTCTTTCGGACTTGTGAAGTCCATTACCCTATT20.14388489208633093No Hit
CTTTAGAATGGGAATTTCCAGATGAGGATACCTCCGTCTTTACCAACACT20.14388489208633093No Hit
ATTTGTGTTAAATGAAATAATACGTTATGAATTTACCACTGATACGGTCA20.14388489208633093No Hit
CGACGATGGAGGGAAAGACGGCTCTGGGAGCGTCATCACCGGCGAAACCG20.14388489208633093No Hit
AAACAATCCAGGAATGACGTATGAGAAATTAAGCAGAGCTATGAGGTATT20.14388489208633093No Hit
GGAAGTTTTAATCAAAATGACATAATCAGAGCAGTGACTTAATTACAGAC20.14388489208633093No Hit
ATTTAAGCTCTATCATGAATCACTGATAAGCCCAGAGCTTATTATAGATA20.14388489208633093No Hit
CCCCGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC20.14388489208633093No Hit
AGATGGTCAAAGGGAGATAACTGTTATACGACACCTTGTTATGCCTGGTC20.14388489208633093No Hit
CTTAGGCTCTCTGTCAAGAACACAAAATAAACTGAGCAGATAAAGGACAA20.14388489208633093No Hit
GATAGATTTACTTAAGAGTAGATTTCTAACAAAATATGCCTATCAAGACT20.14388489208633093No Hit
TGGCTGCCACGGGAAGTCAGGGGGGCGAACCCAGGCATGAAGAAGTGCAA20.14388489208633093No Hit
GCAAGATCGTGTAAGCAAATAAGAAATACCACTCTGGTTGAATGTGGAGA20.14388489208633093No Hit
CAGTTTTATGCCATTTAGGGCTAATAGACAACTATTTAACCTAACCGCTC20.14388489208633093No Hit
ATCGGCAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC20.14388489208633093TruSeq Adapter, Index 12 (98% over 50bp)
CAAACAATACCCAGATGGACATACGTATCTGTATCTTTAACATGCTTAGA20.14388489208633093No Hit
GCCAACAGTTATAAACATTTCCAGTTCCCTCTTCATATACATGTATATAA20.14388489208633093No Hit
TAAGATAATGTTTTGTATTTATGCCACAATGCCATATTGTGTCAAGCTGT20.14388489208633093No Hit
TACGTGCAACATGTCATATCATGCTGATGAATTTGGCCTTATGTTTTTGA20.14388489208633093No Hit
CAATCCTTTAGTTTCTGGTACGTCACTATGCCAAGATGTGAAATGGAACT20.14388489208633093No Hit
ACCCCCGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC20.14388489208633093No Hit
GGCCGACCAAGTAGACAGTTAGCGAGATCACATGTTCTTACAAATCTGCA20.14388489208633093No Hit
AGCATTTTGAACCGAATTGGCAATGAAATTACGCTTTGAAATTACCAATT20.14388489208633093No Hit
CAAAGTCGTTAATATTTTTATTAGACAGTTTGACTCCCTTGGGAGAGATC20.14388489208633093No Hit
TGTGACCTCTGTCTGTTGCATGGGTGGTGCGGCAGCATCTGGCGCCCACT20.14388489208633093No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)