FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-ctenidia-RNA-2_S11_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-ctenidia-RNA-2_S11_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1031
Sequences flagged as poor quality0
Sequence length62-151
%GC40

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC716.886517943743938TruSeq Adapter, Index 12 (100% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC90.8729388942774006TruSeq Adapter, Index 12 (100% over 50bp)
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC80.7759456838021339No Hit
TACATCTAGTATATCGACACAACTTTAATTAAATTCAACTGATTCATTAA50.4849660523763337No Hit
GTAACCCTCGCACGACAAGTGAGGTATGACATCCCAAAAATGTACCTTCC40.38797284190106696No Hit
CCTGCTTAAGACAGGTTTGACTGTATACTAAATTAACATGAAATCAATAA40.38797284190106696No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.38797284190106696No Hit
TTTTAACAAATGATACAGTAAAAACATGAATTCCATTAGACAGTTGGAAA40.38797284190106696No Hit
GCTCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC30.2909796314258002TruSeq Adapter, Index 12 (98% over 50bp)
TTTTGCTATTTGTTTTATATTTTCTTGCATTTTCTTGTCTGGTACTGTTC30.2909796314258002No Hit
TAAAACTGAACGGGCCAACACGTTTTCAGTAGAAATATAATACATTTTAA30.2909796314258002No Hit
ACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC30.2909796314258002No Hit
TTTTTTTTCTCTTTTCTTTAGCTTTATTTTCATTTCAGAACCAGGTGTAT30.2909796314258002No Hit
CTCAAACATCACAACTACCGGTATGGCACTGATACTGCCCTTCCTATTGA30.2909796314258002No Hit
CTCGTGTATTATCCAGTAATACAAAAATTCGTGTAAGAATATATAATTTT30.2909796314258002No Hit
GACACACACGTTTAACAGCGCTGATGACGTAGTTGTTCCTGTACCTACGG30.2909796314258002No Hit
GACACGACAACTTGACATGTTAATATACGAAAAAAAGTTACTAAATATTG20.19398642095053348No Hit
TTATTAAACAGTAATAAAGTACACATAAATAACATTAAAACAGTTTAAAA20.19398642095053348No Hit
GCCAGACGTGACATATCAAACAAACTGAAAGAGAGACCTTGAGGTAAGGT20.19398642095053348No Hit
TTACATAATACTATCGAGTGTATCTTCATCATTATACCATACATTAAATT20.19398642095053348No Hit
TAAACATGGTACATTGTTTTATCAGGTTGCTAATTTTTATCAGGTAATAC20.19398642095053348No Hit
TTTACAACTAATATTGTTTATTCATTAAAGAAGTTTCATCAAAAATATTT20.19398642095053348No Hit
GGTAACCAAGAGATTTCGAGTTTGATTTTGGGTTTGGACTAGGGATAGGA20.19398642095053348No Hit
AACGGCGGCACGAGGACGTTCCGGAAACTCTCTTACATTCATCCACCTTG20.19398642095053348No Hit
GCTGAAACACAGCATGAACACATTCGTCTCAGGGTAACACAGCGGGCGAA20.19398642095053348No Hit
GATTTTAAGGCGATGAAATTCTATAAGATGAGATAAGCTGACCTAGGAAC20.19398642095053348No Hit
GGCTCATTCTGTTGGCAATGCCACCAACACTATCGCCGGGTCCTATCAAC20.19398642095053348No Hit
CACTTTTACAATTTCAATATCTTCGGCAAAATAATAAGAAGCGCTATTAA20.19398642095053348No Hit
CACCGGACATTCGTATATGTTTCTCAAATCCTGCCTGTCTACAGGGATGG20.19398642095053348No Hit
CTTGAACTAGAAACTGACAAATTGTGTTAAACATATTGTTGATCACCAAT20.19398642095053348No Hit
GTTCAACTGGCCAGGGAATCGGAGACAGGTGGTGACTCCAGACATTGTGG20.19398642095053348No Hit
GTAAAATATGAAAACCGTCAGCAGCCAATTTATGTGACATATAACAGGTA20.19398642095053348No Hit
TGGGTTGGTCATCCCCAAACAAACCGTGCAAATTCACTCTGTACATATAA20.19398642095053348No Hit
TTTTTTTTAAATGTATAAAATATTTTATTTGTCAAACACAGCATATTACA20.19398642095053348No Hit
CAGGAAGTAAGATTCCAGGTTCACTCTACATTTACAAAAACAACAAATTA20.19398642095053348No Hit
CAGACAGACCCATGGGGTAAAACATAGTGTCCTTCACCATGAAGACTTTA20.19398642095053348No Hit
CTTTAATGTCACCTGACTATACATAGGTGAAATATCACAGTGATTAGTAA20.19398642095053348No Hit
NATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC20.19398642095053348TruSeq Adapter, Index 12 (98% over 50bp)
GAATGAACCAATCCATGCACAAATGATTCAGTTGACTTTTGTGTGAGATG20.19398642095053348No Hit
CTCTAAAATGCAGATGGTCAGAAATAGATTCCTCTAATACCCAGATGGTC20.19398642095053348No Hit
TGGTTCTTTTGTTGTTGGCCTAGTTTGTGGTTCTTTTGTTGTTGAATTAG20.19398642095053348No Hit
CTTTCCTCAACTCAATTGTTTAATAAATGGTATAATCATTTACATTGATC20.19398642095053348No Hit
GAATGTGTAGTCAAAAACAAGTGTTATCCAGGATCTGAACATGTACAGGT20.19398642095053348No Hit
TTAAGCACTATTAAGTACAAGCCATTCATTAAAGCATGGATATATGCACT20.19398642095053348No Hit
CTCCATAGTACTGTGCATCCAAGTAATTGTGAAGGGGTTCGGGACTGCCT20.19398642095053348No Hit
CCAAAATGTCCATAACATGCAGTTTTCTGATACATAGGGTTCCTCAAGTT20.19398642095053348No Hit
CCCAAGTGGAGTGGGCTATTTTGCCTGTAAACCTACGTTTAAACAAAGAA20.19398642095053348No Hit
AATAAACACTTGATTGTCATTGGATGAATTATTAATTAGACAATAATATG20.19398642095053348No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)