FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-ctenidia-RNA-1_S3_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-ctenidia-RNA-1_S3_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3627
Sequences flagged as poor quality0
Sequence length35-151
%GC37

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2476.810035842293908Illumina Single End PCR Primer 1 (100% over 50bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT371.0201268265784396Illumina Single End PCR Primer 1 (100% over 50bp)
TTCAATCCCTGCAAATATAGAAAAAAATTCCAACATGCAAAATTTTATAT130.35842293906810035No Hit
GTCTGTATCACCGTCTTATTTAATGGACAGCCTACATCAAGGGAGACCAC120.3308519437551696No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.3308519437551696No Hit
GACATAGCCAGGTTATATGTAGAAAACAAAATCTTTACACTGACTGTACA120.3308519437551696No Hit
TTTCAGTCTGTTTGAGATTTCGTGTCACGCCTGTAACTTTGTCATGCATA120.3308519437551696No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC110.30328094844223874No Hit
CTGAGTTGTTAGCTTAATTCATTTTACAAAATAACAAATCCTTCGTTCAT100.27570995312930796No Hit
ATTCTTAAGGTTTACAATTCAAAAAGATTTAGAGCTGGTAAAGGTAAAAT100.27570995312930796No Hit
TTCTAGTGCACTTTTTAGAAAGACAAAAAAAAGTAACTCTGGTTGTGTAA100.27570995312930796No Hit
TGGTTTATGATTCGAGGAATCAACCAGTTTATTGAATATGTAGATACAAT100.27570995312930796No Hit
GTTTGTTTACAAGTTGATTTGATGGTGTGTAAATTTCATAGAAGTTTGTT100.27570995312930796No Hit
GCCAAATGTTTTACATGTTATATGCTGAATGAAGATTATTCCAACTGTGA90.24813895781637718No Hit
CTGAATTGCCGCAAAATGTTATGGATATTGATTCTGAAATTATATATACA90.24813895781637718No Hit
TGGCATTGGCCCAGCATGCACAAAACATCACAAACACAAAAACTACAGAC90.24813895781637718No Hit
ATGTTGTAGTTTCACAACTGCATATAAATATAAAATGATGTTTGAAAATT90.24813895781637718No Hit
AAAAGACATTTTAATACGCAATTTAAAAACATCTTATTTTCTAGGATAAT90.24813895781637718No Hit
GCAGAATATGCTGAAACTTATTGTCGGTATGAAGATTCTAGGTTCATTTA80.22056796250344637No Hit
GGACTCATTACAAACATCAGCTATCTAAATATTGAATAAAATAGCAATAG80.22056796250344637No Hit
GTTGTACACAAAATTTGTAAAATCACACACACATTAACAACAAATATTTG80.22056796250344637No Hit
TGCTATGCCTGTGTTGGCGGGGGCTATTACAATATTATTAACAGATCGTA80.22056796250344637No Hit
GGCAAGTAACTGATAACTTTCCCACTTGAATCAGTGGTGAGAGATGAATG80.22056796250344637No Hit
GTGCGTGCGTGCGTGCGTGCGTGCGTGTGTGTTAGCGGGTTCATCTGTGT80.22056796250344637No Hit
GAAGTATATCGCCTGCTAGAGGTCGATAGTCGGTTTGTTTTGCCTAGAGG80.22056796250344637No Hit
CTACACTAGACCATTGTCTGTAAGCTTACAACCGAAATACTGTGATTGAC80.22056796250344637No Hit
CAGCATCAGAGATTTTTATATTTAATATTTGATTAAGGTCTTATTCAGGA80.22056796250344637No Hit
AATATGACTGCCAACCCAGGGGTTGTGAGTTTGAATACTCGCCCACCACA80.22056796250344637No Hit
GCCAGACCTTCAAATACAACACCAAGGTCCGATCTGGTAGAGGTTTCACA80.22056796250344637No Hit
GGCCGTGCCAACTGCAGATAAAGTTCAGGAAATCAGGGAGGTGACAAGAA80.22056796250344637No Hit
GTGTTATAGTAAATGGCAGACATTAAGGGACAATTGTGTCGGTTGTCTGG70.19299696719051557No Hit
CTTCTAAAGGAAAGGAAACAATAACCTAAATAACATATTTAACCTTTCAA70.19299696719051557No Hit
GGAATTGACACCCGTGGGTTTCCGACGATGAGCTATATCCGGAATGCTTC70.19299696719051557No Hit
GCCATATATAACCTGAAATGTGTTGAAAACGGCATTAAGCCCCATTCCAA70.19299696719051557No Hit
CTTATGTGAACTTCTATTGTTATCATAGAAAGACATGAAATGGACAAGAG70.19299696719051557No Hit
CCACAGTTAGCAATAAAAATGTGAAGCAAGTGTATTGTACTGAAAATGAA70.19299696719051557No Hit
CTGGGTATAACTGTCCAATTAAGGAGCGGATGCTGTACTCCAGTTGTAAA70.19299696719051557No Hit
ATAAAGACATTGGAGTCTTATATTTTATCTTTGCTATTTGAGCTGGTATA70.19299696719051557No Hit
ATTTTAAACAAGATTTCACCTAAGGCCATGTTAAGATAATAGATCTTTGA70.19299696719051557No Hit
GTGCATTAATGTTACACTACACAAACTATTTATACTATTGTTGTTCACTA70.19299696719051557No Hit
TCAAAATGAGAGAAATCGTTCACATGCAAGCCGGCCAGTGCGGTAACCAA70.19299696719051557No Hit
GTTTTATTTACACATCCGTATCTGACATCTGTGACAAATCTTTGTATTGG70.19299696719051557No Hit
CAACACTTTGGAACTTTACAAATATTGACATAATCACTTGAAATTCCTCT70.19299696719051557No Hit
GTTTTGACCTGTATTATAATAAGTTTACGTTTTTGAAGTGACAATTTGTG60.1654259718775848No Hit
GCAGTCGGAGGGGCAAGGGGGTGATTCAATGTTGTGAATAAAATATTATT60.1654259718775848No Hit
AAGAATCCAAGCAAGGAACCAGTCCATTTTCCAACAAAATATGGAAATCC60.1654259718775848No Hit
GGTGAGGCCCTCCTCAATTATTCTACAAAGTTTCATATGAATTTATTTAT60.1654259718775848No Hit
CTACATAGCCCATGGAGACGACAAAGGCAAACTGTGCGTTGTTGTGGATG60.1654259718775848No Hit
TGCAGGTTGGGGTGTCATGGTATCACACAGGAGTGGTGAGACAGAAGACA60.1654259718775848No Hit
GTCCATCCGTCTGTCCATCCTTCTGTCCATCCTTCCGTCTGGTATTTCGT60.1654259718775848No Hit
CTTGTACATACATTAAATGGTAATGGGTTTGGTATATTTCCATTGTCATA60.1654259718775848No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN60.1654259718775848No Hit
AGGGTGTCACTTTAGGTATAGAGTGCATGGCGAAAGATTGTGAGATGATG60.1654259718775848No Hit
CCATTCTTTACATATTCATTTTTAAACATTGATGCATAATTATGCAAATT60.1654259718775848No Hit
TGAACTTTGGTATCTGTTTTTAACCTGTTCTCCTTTTAAAATTGATGGAA60.1654259718775848No Hit
TGTAGTTGGTGTTTGAGTAGCTATATTTGTAATCAGATATGTCTATGTTA60.1654259718775848No Hit
TGACATGAGGCAAACCGTGGCCGTGGGTGTCATTAAGGAGGTGACAAAGG60.1654259718775848No Hit
TTTGGAGTGTTGATGTGGGAAGTATTTACAAACGGTTTCCTTCCCTACCA60.1654259718775848No Hit
TGGATACATTTATTGGGAAAACAGCTCACGAAATTGCCAAGAAGAAAACG60.1654259718775848No Hit
TGCTTAATAGAGGTGACCGCTTATAAAGGTTTGACTAGTTTAAAAATTCA60.1654259718775848No Hit
CACGAGCAGCTCTCCGTTGCCGAGATCACCAACGCTTGCTTCGAGCCAGC60.1654259718775848No Hit
CAAGAACGACATCAGCTACTACCACTCCGGCTGAAACAACAACGACATCA60.1654259718775848No Hit
TTCATTTCTCATGTGATATATAACATTTTTATATGATTTAAGTTATTATT60.1654259718775848No Hit
TACAACTCATTTTGTTTCATATGAAGACGTGTTATCTTGGTGAAATGAAA60.1654259718775848No Hit
TGTGGCCATTCAAATTGAACACGCCTAATGGTGGCTGGAAACGAAAGTAT60.1654259718775848No Hit
GATACCTATAATAGTGGGTGGTTTTGGGAATTTTTTAGTTCCTTTGATAC60.1654259718775848No Hit
TTATATTTGTGGTGTATGTCATAACTGTAAATAAATGTGACAATGATAAA60.1654259718775848No Hit
TTCAGGATCCCAGGAAACAATGGTAAGGTCGATGGCATAAAAATGACGCT60.1654259718775848No Hit
GTTTTGAGTATTTGCCTGTTGACAGATACTTATATACAGAAAATTTAGGG50.13785497656465398No Hit
GCCGACAAGGCTTGGAACTATCCTCTAAATGCTGAGCACCAAGCGTGGAA50.13785497656465398No Hit
GGGGGATTCAGGATTCCCGTTTTTTTAGCGGGTTGTGGTTTAAGCTCCCG50.13785497656465398No Hit
GGACCTGGCACCAACATCTGTTGTATTGTGTAATATGCTATAGTTAGTTC50.13785497656465398No Hit
CATGCGTTTATGCTGCCCTCATCCTCGCCGATGACCAAGTAGCCATCACT50.13785497656465398No Hit
GCTACATCGCTGTTCTCGCTCAGTAGATTTGTCTTAACACTCATTCCAAA50.13785497656465398No Hit
GTCAATATGCTTCCACAATTGGTCGACAATAACAACAACAGTGTAAAAGA50.13785497656465398No Hit
TTTAAGTTTAGTTGTTTATTTTATTGCTTCGTCGGACATGTTCCCTTTTA50.13785497656465398No Hit
GGTCAATGATCGATGGTGCAGGCGCATACCACCGAAACTGATATACACTG50.13785497656465398No Hit
ATATCTACAGACAAGAAGGTTCGGATATTTAAAGTAATGACTGGAAAGAT50.13785497656465398No Hit
AAAGATGAATGGTATGGGAATTTAAAGTATTTCCTTTTCATAGCAATTAA50.13785497656465398No Hit
AAATCTGGTACTATCTTCGATAACTTTTTAATAACCGACGATGTTGCTGA50.13785497656465398No Hit
TGTAATTCAAGAGAAAAAAGTTCCATTCCATGTCAATATATTATAATCAT50.13785497656465398No Hit
TATATATATAACATTGTAGAAGCTAGTTATGATGTCCAAAGAGATACATG50.13785497656465398No Hit
TGGTATTGCTCTTGCATTCATACAGATAATCGGAATCATCTTCGCCTGCT50.13785497656465398No Hit
GCAGTTAATGTTTCAGGATAGGGCTTCTTTGACTATAAAGAATTTAGTTC50.13785497656465398No Hit
CTCGAGCACCCTCAATCTCAGCAAGTAACTGATAACTTTTCCACTTCAAA50.13785497656465398No Hit
GAATGGAAGATATTGGTCAAACTGTCTCAGAACATGTGGACACTGTAAGA50.13785497656465398No Hit
GGCAAATCTGATATTTGGAGACATGTTGGCCTCAAGCAAAGTCGCGGTAT50.13785497656465398No Hit
GGCAACTCCTACTAAACCTGTGGGCATGCCGACTGAAAAAAGGAAACCAA50.13785497656465398No Hit
TGCATACCAAGTGAGAGACTTTTGTGATCTTTCTAATGGACCATATGCAG50.13785497656465398No Hit
GTGATCCTCGTCACGGCAGATACCTTACCGTCGCCGCCATGTTCCGTGGA50.13785497656465398No Hit
TGGGGCTTCGTTGTTTTTTAGTTGTATATACATTCATATTGGTCGTGGAA50.13785497656465398No Hit
AAACATTATGAGTCGACCTGTACCATGATATTACCTATATGTCCTCAAAC50.13785497656465398No Hit
TGAGACTGGCCGCTGGAACATGAAAGAAGTACCAGGTTCAGAGAAAGTGT50.13785497656465398No Hit
GCTGTCTGTTATTCAGCAAAGGCCTATCATGAGCGGATCTGCTGGGATGA50.13785497656465398No Hit
AACCAAGTTAAAAAATACAATACATACACGGACATACTGTTGCTTCATTG50.13785497656465398No Hit
CGGTTGACAAGGTAGCCCAAGATGGAGCTGGCCTGGCGTTTGTTGTTTAT50.13785497656465398No Hit
GAGATAACAGAAATCTCTGTTTAAATTATTTACACAATGTTAATGAAGTA50.13785497656465398No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.13785497656465398No Hit
GTTGTCTACCGTGTGCGTGTTCGCCGTGGAGGACGTAAAAGGCCAGTACC50.13785497656465398No Hit
CAGTGATATAAGAGTAGTTCCAGCCCTGCGAGGTGGGCGTCAGTTTTGAA50.13785497656465398No Hit
TTTATGAATATATGTATTGCTGTATTTTAGAATTAAATGTTGAATATTAA50.13785497656465398No Hit
GATTTATTCCAAACAGCCAATCTAACGGACAGAGGGAACGGATTCCCGCA50.13785497656465398No Hit
TTAACATATACTTGATATGCTGACTGTGGGGAAATAAGACTTTTTTATGT50.13785497656465398No Hit
GTTTGATCCACTGCCACGGCAATGTATTGTGTCCTTGAGCTGAAAGACAC50.13785497656465398No Hit
GTCCAGTCTTCCTTTTGTCAGCTTTACCACCCTAAATTCCACGCGATTGT50.13785497656465398No Hit
TGCATACTTTGTATACCAGTAACATATCAAATGAGTTTCATGACTTCAGT50.13785497656465398No Hit
AGAACACACCAATATGGATAGTGATGACCTTGTCCCCAGTCTACAGGCTG50.13785497656465398No Hit
CGAAAGGGGCATTCAATAGAGTTACATTTTTTTCCAATTCCACAACTCTC50.13785497656465398No Hit
ACTTGATTTTACTGAAATATAGGAATATAGCGATTAGGTAAATGAAACGT40.11028398125172319No Hit
TTGGTTAAGTTATAATATAGACAAAATTAGCTGTGATATTTTAACAACAT40.11028398125172319No Hit
AAAACTTCAAGAACCCTGAAACCATTCTCCTGTTAGCGTTTGCCATCATC40.11028398125172319No Hit
AAGTTGGACACCTCAATATTATTTTGATTTAAAGTCGGACATGCCATAAT40.11028398125172319No Hit
GTAAAACTTGTGATCTCGAAAAAAAATGGCAGTACATTGAATATGTATGT40.11028398125172319No Hit
CACGATTGTGGAGCCTCATCAGTTGACGCAACAAAAGGTTGAGGAGAGGC40.11028398125172319No Hit
GTTTAGCGGTAGTTCATCTTATTTTTCTTCATCAGACAGGGTCAAACAAT40.11028398125172319No Hit
TAGAAATGCAGGCTAGTTTTAAGTTTTATGAACATAAAGTGCAAAGTTTG40.11028398125172319No Hit
CGAAAGTTGTGGAGCATGTGAAGTGTACAGGGACAGATCAAGTGGACAAG40.11028398125172319No Hit
AAACAGTAAAATGTTTCACATCAGAAGTATTCTAATGATTCCAGGGACAC40.11028398125172319No Hit
CCGCCATTGCCACCATCAAGACCAAGAGAACCATCCAGTTTGTCGACTGG40.11028398125172319No Hit
CTTCTCTCCCAGAGAAGGTGTTGCATAGTTTGTATTTCTCCAGTTTAGAT40.11028398125172319No Hit
ATTTCCTGAAAATGTAAATATTTATTAAAAATTGATTAATACATCAATCT40.11028398125172319No Hit
GTTGCATTGGTTATTTAAGTCAGACAAATAGGCCATGTTGTCCGTATGCA40.11028398125172319No Hit
GTTCCATTCATTATTGAAATTGCTAATTTGAGGTTAAGATTTTAAAATTT40.11028398125172319No Hit
CCCAAAGGAAACAGTTAGATTTATTTTGACATTCGCTTGTAACGGAATAA40.11028398125172319No Hit
CGGGAAGTTGCTCTCCGTCGTACATAATTTTGGACTTAAAGTCGGCTTCG40.11028398125172319No Hit
ACCAAGTCCATGGATCTGTGGACCGGTCACTGTCAGAAAAAACTAAAGGC40.11028398125172319No Hit
GCTGTCTGCGTTGGAACAGTTACTGATGACTTGAGGCTTCTACAGGTCCC40.11028398125172319No Hit
AAAACAATAATTCAACCGTTGACAAAGGAAGGTCTCGCTAACAACATCTA40.11028398125172319No Hit
TGAAAAATGAGTGAATAATTTAAAATGGCATTAATTGGCTAAATATATTA40.11028398125172319No Hit
CCGAGATCACCAACGCTTGCTTTGAGCCAGCCAACCAGATGGTGAAATGT40.11028398125172319No Hit
CTCAAGTTGAGATTTTCCTCAATTCCATTCTTTGAAACTGCCACAACTTC40.11028398125172319No Hit
TTCAGACCCTTGCTAGTTACAGCTACAGAAAATGGTCCAATTGTAAAAGT40.11028398125172319No Hit
TGAGATTCGATGGTGCCCTCAACGTCGACTTGACTGAGTTCCAGACCAAC40.11028398125172319No Hit
CTTATCTCTAAATAAATAAATAAAATAAAATAAATAAATTTTTTGTCCAT40.11028398125172319No Hit
GTGGAACAGATTGAGAGCAAGTGATAGTGGATCGTTAATGAAAAAACATC40.11028398125172319No Hit
CTTAACGCAACATATATACCCATTGCATAAATCTCAAACATAAAATATAT40.11028398125172319No Hit
CGGCAACATAACGTCTTTGGTCATTTATAAAGTATAATTACAAGAAGAAT40.11028398125172319No Hit
ATTGATGTGTTGGTACTTTGTAAAATCCTAATTGTTTCCTGACATGCTCA40.11028398125172319No Hit
GGAAGCAAAACAAAGTTGACAGTATACTAACTTTCCATATTACGTGATAA40.11028398125172319No Hit
GCGATGTTGTCTCTTCGGCAGATGCTCCGTGCTTCAGAAGATTTCATGTT40.11028398125172319No Hit
ACTAAATCTATAAATCATGCTTGCAGCTGGACGCCGATTGTTTTCGGCTG40.11028398125172319No Hit
CAGATGACAAGTCCCAAAATCAACGCCATCGTAAAACACACGGGACATAA40.11028398125172319No Hit
GTACACTTGTCACCAAGGCTGGGCATTGACTTTGACGTGCTTTTCGTCAG40.11028398125172319No Hit
TTTTTTTATGTAAACACTATAATCTTTATTGGGACAATGAATTAACTGTC40.11028398125172319No Hit
GTGGTGTCCTTGAGCAAAGACATTTCATCCGCATATGCTTAATATTGGTT40.11028398125172319No Hit
CTACAATTGATGGTGCATATAATACTTGTGGTGTACACTGAAGGTGTGTA40.11028398125172319No Hit

[OK]Adapter Content

Adapter graph