FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-ctenidia-RNA-1_S3_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-ctenidia-RNA-1_S3_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3627
Sequences flagged as poor quality0
Sequence length35-151
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC2386.561896884477529TruSeq Adapter, Index 12 (100% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC340.9374138406396471TruSeq Adapter, Index 12 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130.35842293906810035No Hit
GATTTTGTTTTCTACATTTAACCTGGCTATGTCATTGTGCAGTCAGTATA120.3308519437551696No Hit
ATCAAAATGTCCACATGACCATGCCAGCCCCCCGGTACACCAATACTTGT120.3308519437551696No Hit
GTGTTTTTGAAATTTATCCTTAGGTGACAAAGTTTCAGACCGGACAGGAA110.30328094844223874No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110.30328094844223874No Hit
CCAGGAGCAACCTTCAGCAGATTTAAATGTGCAACATTTATCAGAGTGAT110.30328094844223874No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC100.27570995312930796No Hit
ATTTTTTATAAACATCACAAAAGGATCATATTAATACAAAATAAATAAAA90.24813895781637718No Hit
CACCATGTCAGTTTAATCTCTTATATAAGAAGTATTTTACATATACATGT90.24813895781637718No Hit
CTGGTTTATAGTTAGATGTACTGGTATATAGTTAGGATATGAATATTGGT90.24813895781637718No Hit
GTGCTTTCATCAAACGAAACGATGCTTTGATCTGAACCATTGGCGGTCCA90.24813895781637718No Hit
TACTGAAGATCCATAGTACCACTGTACATCACTTGAAAATTAAATTAGAA90.24813895781637718No Hit
TGACATCGGGTGGTTTGAACAATCTTGTGTTTTGAAACAGTTTAATTCTT90.24813895781637718No Hit
TTTGACAGATGAGTCACAGGCTCGAACACAAGGAAGATAGGCAAGCTATC90.24813895781637718No Hit
TTTTTGTCCAGAAGATACAACGTAATGTTTAATTGGATATAGTTAACTTG90.24813895781637718No Hit
ATAGAGCGGTTTCTGCGACGATAATCAACAGCAATTCCAATGGTCATAGC80.22056796250344637No Hit
CCTCAATCTGCCCCACCATACCCTGGGATACATTGCGTGCCTCCAAAGCA80.22056796250344637No Hit
ACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC80.22056796250344637No Hit
AAGGGCTAACAGACGATCAGTTAATCTAACCACTGTTTCCTGGGATCATA80.22056796250344637No Hit
TGAAAACAACAGTAATATGACCCCAATGTAAGAAGAAGCAATAAACTCCC80.22056796250344637No Hit
GGAAGAAGTCATAACAGCGTCAGAATTGCCAGCATGCGCACGGTGTTTTT80.22056796250344637No Hit
TTAAAGTACAAAACAGAATATGAGTTGTAACAATGGTGAAGCTGTACAAG80.22056796250344637No Hit
ATATATTCAATTTTAACATGAATATGTATAATTCATTAAAGCAAATAGTC80.22056796250344637No Hit
CGAGTTTTAGTTAACTTTATCAAAATACATATATTAATCTGATAAAGTTA80.22056796250344637No Hit
CCCGAATGGTTAAATGAAATAAATAAAATATCCTGATGTCCGTCTGCAAA80.22056796250344637No Hit
CATAAGTTCACTTCAATAATCTTTTATGTATAATAACCATTAAATTTAAT80.22056796250344637No Hit
AGGCAATGATGATAATGGTGAGTCCAAGATTCTGCTGACTGGCGATAAAT80.22056796250344637No Hit
CGGAGGTATCGGCCTTGTCGACTCTTCCTTCAACGCCTTTCCCTTTACCG80.22056796250344637No Hit
AAATTATACAAGCAAATTGTGGAAAAGTATGAAAAACATATTTAAATATA70.19299696719051557No Hit
CGTGCAGCTATTCGTGAGTGCAACATGATGCAGATTTAATATTGTATAGG70.19299696719051557No Hit
ATTGGACATTGAAGGAGAAGTCAAATAACGCATGGTTTTCATTTTTGCAT70.19299696719051557No Hit
TTAAGAACAGCAAATATACCTTAGTTTGACATCGGTTGCATTTATGTATG70.19299696719051557No Hit
GGGAGATAATACCAAACCCAGGAAGAATCAGAATGTATACTTCCGGGTGA70.19299696719051557No Hit
ATATTACAAAATACAACAGATGTTGGTGTCAGGTCCGTGTGGAACTAACT70.19299696719051557No Hit
CAACTTCATGGTTGTATATCAATTTTCATAAAATTTTCATGATGCCTTCC70.19299696719051557No Hit
TCAATCTGAGATCTTCATCCAACAGTAAAACTGGATAGGCAATACATAAC70.19299696719051557No Hit
ATTTTTCTTTGGGTGAATCTCATCATACAAGTAATCTTCTGAAAGTTCCT70.19299696719051557No Hit
TTATTATTTACAATTTTACCCGTGAACGGGTTTCCAACATTTCTGTGAAA70.19299696719051557No Hit
GGGGCATGGTACTCTATACCACATTTAAAACAAAAATTAGTTTTACAAGT70.19299696719051557No Hit
CAGTTTGCTAAAGCCTTAATGTTTTTTATAACAATTTTCCTCAATCGCAC70.19299696719051557No Hit
GAGGATGTGCAACTGCAAGACATGCCAACACAAAAAAAATATATTATGCC70.19299696719051557No Hit
TTTCATTATGTTCCATGAATTGAATCAGTATTTCTTGATGTTCCATGAAT70.19299696719051557No Hit
GTTACTGATAGCGTAGTTGTTTCAGCCGGAGTGGTAGTAGCTGATGACGT60.1654259718775848No Hit
TTTCACTAGGCCTTGTTTTCATTGAGCCTCTTTTTCACTAGGCCTTGTTT60.1654259718775848No Hit
GGAACCAGTCTGTAAAATGTCCTAAATTGTGTTAATCTGATAACAGGACT60.1654259718775848No Hit
GTTTCACTGCACTGTATTTTACATTCCATATCACATACAAATGTTACATA60.1654259718775848No Hit
AAAAGTAACAAAACTGCCATAGGTAAAAGCCAAAACCTCATGTTATTTAT60.1654259718775848No Hit
GGCTTCTATTTGAATCTGCAGCTCAGCAAATGTCTTGTGCCAGTTCAGGA60.1654259718775848No Hit
GGGGCACCAGTCTTGATCTGACCAGTGCAGAGTCCAACGACAAGGTCAGC60.1654259718775848No Hit
CTCACAACACGTCGTCATGTTCTGTTGATCATTTGTTGCAAGTATCAACA60.1654259718775848No Hit
TTATAACAAAGGCATGCGCAGTCACAATTACATTATAGAGATGATCATCT60.1654259718775848No Hit
TTCTTCTTTCCAGATGCCTTCATGGCAGCTTTCGTTTTCTTGTCCTCAGC60.1654259718775848No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN60.1654259718775848No Hit
GTCAGGGTGACATCTTGTTGCTTAACTGAAAGGAAGTTTTCCACTCCAAG60.1654259718775848No Hit
GTTTCACCAACATTTTAGTGTATAAAATTTTACATCTATTGAAAGCATCT60.1654259718775848No Hit
AAAAAAATCTAAAAATCAAATGAAAATTGATCTGAGTAGAACAAATGTTA60.1654259718775848No Hit
AAAGAACACCAGCATTCAAAATACTGTTTCAAATTATGTAATACTACAAG60.1654259718775848No Hit
CTGTGATGTTGTCCTTCTCCCAGGACTTTTTCACGTATTTAGCTTTGCAG60.1654259718775848No Hit
TTTTGCTTTAACTGCTGACATTCGAAACGAAATACATGTTTTACCTGTTG60.1654259718775848No Hit
ATTCGTCTCAACAATGGGTTAATCTGTGTGTCTCTGAGTCCACAGTCACC60.1654259718775848No Hit
GTGACTTTAATGTCGTTAATGTGTCTTGCAGCACCATACGCTGTCATGCC60.1654259718775848No Hit
TCTAGAACCAAGTCCTTAGAATAAAACCCTGCTATAAACGGAACCCCTAT60.1654259718775848No Hit
CAACTTTTAAATAATAATGATTTTGAAAAACAAAACAAAATATATTCATG60.1654259718775848No Hit
CTTGAACTCAAGAATGGATTTTGAAATTGATTTTGTATTCAATTAAACTC60.1654259718775848No Hit
ACAACATCACCTCTGTACAACATACAGCAGGCCATGTACTTGCCGTGACG60.1654259718775848No Hit
TGGGAACCTTGGCATATACAAAATATTTCCTTGGCAAAACTAAACTTGTT60.1654259718775848No Hit
ATTCTCACCCTGGGCTAAGCTCAACTGGTTGAGCAAGCCAGACTGATAAG60.1654259718775848No Hit
TTTTACATACCAGTAACTGTTTTTTAATGTGTTGAAATCTTGCAAGTTAA60.1654259718775848No Hit
GTCACGGTCAATATTTACTATATTATCTTGTCACGGTCAATATTTACCTG60.1654259718775848No Hit
GTCAGAGTCGCCGTGGTAGGTACCAGTGGGGTCGATGCCGTGCTCGTCGG60.1654259718775848No Hit
GCAAATCTCAGAACACTGCCCATACACTACACCAACATGATTTCTCTTTA60.1654259718775848No Hit
TTCACCTCTTTCGGAATGGCACGCCTCTATCACAGCAAATATATCGGTAG50.13785497656465398No Hit
CCCTTGTATGTTGAAGGACCTTGTCAGGACCATCTCATCTCCCACAGATA50.13785497656465398No Hit
CTCACGTTCCCGCTGCAACTGATTTTTCACATCAGTTAGCTCCTGCTGAA50.13785497656465398No Hit
CACTAACCTGTTCTTGACCTTCACACGTGCGCACTTAAATTTAACCACTG50.13785497656465398No Hit
CTTGCAAAGATTTAAATGAAAATGAACAAACAATTGGAAAATGATCAGAT50.13785497656465398No Hit
GTAGATCGCAGATCTATACATTACCATATCATTATTATAATATTGAAGCA50.13785497656465398No Hit
CTTCTCTTCTGCTTTGGTCTCCTCTGCAGCACCTGCGGCTGGGGCTCCAC50.13785497656465398No Hit
CAGCAACATTTTGGAGAGATCGCTGAAACTTCAGTTGGTTGACTCCGTGT50.13785497656465398No Hit
TTTTTTATCTATGTTCCAAACAGAAGAATTTATACTACTTCGTATTCCTT50.13785497656465398No Hit
TAACTTGATCAAAAACGTTTCTATGAGTATAAAGTATAATCTGAGATTAA50.13785497656465398No Hit
TAAGATGGCATCCTAAAATCACATAGCTAGCACATGTTTGTTGTAGAGAC50.13785497656465398No Hit
ATAGCTGGTTTTGCTTTAACAATGCCACCTTCCTTTGAACAGCATTCACT50.13785497656465398No Hit
TTTCCTTTCCTCTTCATCTTGTTCATCCTTCATTTTCTTCTCGGCATCTT50.13785497656465398No Hit
CTCCTTCCTCCATACCCTCTCCGACATACCAGTGGACAAATGCTCTCTTG50.13785497656465398No Hit
TTTTAACAAATTATATCAAAATGTTTGTCTTCAAACTCAAAATTGTTCAT50.13785497656465398No Hit
GGATCCTGGAAGATTAACTAGAACGCAAGTACACCCATCTCCACAAGTAA50.13785497656465398No Hit
AGACAAATAAGGATATCAAATTTTGATTTTCTCTTCGAATATTGCCAGTA50.13785497656465398No Hit
CTCTTCTGACTCATAGTGGCCATAGCACTTTTTGAAATTCAACAATATTT50.13785497656465398No Hit
GTTTTAATTGATCTTTGTAAAAACAACATTTACATTTAACCGCAAAACAC50.13785497656465398No Hit
CCCGCATCAAATTCCCATTAAACACGAAAATAACTAAAAAATACCCGACT50.13785497656465398No Hit
GCCAACCTTCGTCCAAACTCCATATTAGGCAACTGTTGCTTCATCTGCTG50.13785497656465398No Hit
GGTGAACATCACAGAGTTGCTGTCAGATGAGACATAAAATCAGCGAAAAA50.13785497656465398No Hit
GTCAGCATGTGTAATTCCCGGGAGCTTGAAAGAGTCTTTGAATCAGATAA50.13785497656465398No Hit
TAAAAAAGCAGCTATTATTCTCAATAAAAAAATAGTAGTACCAACCCACC50.13785497656465398No Hit
AATATGTATACCATATCAGTGCTCATTTGATCAAACTTAGTAATTTATAA50.13785497656465398No Hit
GCCCAGCTGACTTCAATGATCTATCTAAAGCTGTCTTTGTAATTTAAATT50.13785497656465398No Hit
GTCCATGGTAATGTCTTGCTTTAATCCTTTGTTACCATGGTTGCACCATA50.13785497656465398No Hit
AGGTCGACTCATAATGGTATAACATGTTTGAGAACATATAGGTAATCTCA50.13785497656465398No Hit
CTGCACAGCAGAACAATGTTTCACACATGATGTATGTATTTGATCATGTG40.11028398125172319No Hit
GATCGGCAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC40.11028398125172319TruSeq Adapter, Index 12 (98% over 50bp)
GTTCTGCACGTTCAACATCTGTTCGTCGACCTCCTTCATGGACATACGTC40.11028398125172319No Hit
CAATATTGTACTTCAATGGACTTTAAAAAAATCATTTTCAATTTTTTTTT40.11028398125172319No Hit
ACTACAGCTCCATTTTTCTACGGGAAGAGTATGTCAATGTTATATACATG40.11028398125172319No Hit
AGATGATATCCATGTAGTGGTGATCAGCGTGTTACAATTTAATTAACATC40.11028398125172319No Hit
TTTGATACTAATTTGATAAAACACAAGATATGGTACCCTTTCTCTGGCAG40.11028398125172319No Hit
AGAAGAAAATATTAAACTTGTACTTGGTTAAAAAAAAGATACAATAACTG40.11028398125172319No Hit
CCCCTCACCACCCAGGGCAATAATTCTCTTTAACTCCTTGTCCACTTGAT40.11028398125172319No Hit
TGATTCTGCACACAGTCAAAGGTATTTATTGCTGTATTTCTTTTCATGCC40.11028398125172319No Hit
GTTATGTCTCAACACAAGGGAAATGTTTGGGGTTTTTTTGAATGGGGTTT40.11028398125172319No Hit
GTATTCTTGGTACTCTCATCAAGCTGGTCCAAGTAGTTGAACATGAAATC40.11028398125172319No Hit
ACAAAATAAAAATCTTGATATATTATTAGTTTTAAAACAATGTCCAATGT40.11028398125172319No Hit
GTTAAGTCAATAACTATCTTTTACAAGTGTTTAAAACTCATCATTGAGCA40.11028398125172319No Hit
CTTGCCTTGAGTGAGGGTTTCTTCGTCAAAGTGCCTAGGATTAAATTGGC40.11028398125172319No Hit
TTTCAACCATGTTTATCAGGCAACTTTAACCTAACACAAATACATTTAAG40.11028398125172319No Hit
TACATATATGCATATGAGACTTCAGAAATTAAACATTTCTTTACAAAGAA40.11028398125172319No Hit
NATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC40.11028398125172319TruSeq Adapter, Index 12 (98% over 50bp)
TAGGTTATGTCACTTACACTTCATACCATTTCCTGCATGATTACATTGTA40.11028398125172319No Hit
CCGCCGTCCCGTCTGCGTCCGTAGCTGTTACAGTGCCGACAGTGGTTGCT40.11028398125172319No Hit
GGCTACACAAAACTTTCCCTCAATTCAATTTTAAAAACATTAGTGCCCAA40.11028398125172319No Hit
CACGGGCCTCGGAGAACTCACCCTCCTCCATACCCTCTCCGACGTACCAA40.11028398125172319No Hit
TATTTGGTTGTAAAATCAATTCATAATACACTTTAACGTTAATAAAGATT40.11028398125172319No Hit
AAGATCTTGGGGACGGACGGATGAAGCGGCGACTATATGCTTCCCCCCTT40.11028398125172319No Hit
TAAAATAAGCTCGCCTATCAATATTTATGCCAACCGTAAATATGTGATGA40.11028398125172319No Hit
GTTCGGATTTCTCGACTCATCCGTGGTCAATATTCCATACAGAAGGGCAG40.11028398125172319No Hit
TACATGTTGTGTTAAGCTGTATATATTTTATGTTTGACAGTTATGCAATG40.11028398125172319No Hit
CCCTAGTAAATCCTTGTCCACATCTTCATCATCGTCAATACCTCGGAGAT40.11028398125172319No Hit
CTAGACCTAAAATTATCCTTTAAATACTGAGTGCCAAGCGAGGAAGCTTC40.11028398125172319No Hit
CGAGAATGTGTCGTAGGCATCAGGATCAGATGCGTAAATTCCACAGTGGC40.11028398125172319No Hit
CTTTTGTCTTGACCACTCACAACCTCCGGCATGCCTCTTAATTTTTCATC40.11028398125172319No Hit
CGTAATTGTCCATGATTTGCAATAGATACATCCCTCCTGAGCAGGTCAAT40.11028398125172319No Hit
GTCCAGTTTAAGGGTCATATCCTCCAGAATTTTGCGTTCTGGTCCAAGGA40.11028398125172319No Hit
TTCCAACACGTTATTTGACAACCAAAATTACTAGCGGAGGAATCGTAAAC40.11028398125172319No Hit
AAGACTGTTATTTTACCAATTTGTGTTACTGTTCCTTTTTACAAATTGAC40.11028398125172319No Hit
GATATGTCCACTTTAAGTCTTTATTGATTAAATTGTTTAGCTGTATGTCA40.11028398125172319No Hit
TTGTCCTGTCTGCTGGAAGGCTTTGAAGTGTAACTCAGTTCTGCGACACA40.11028398125172319No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)