FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-ctenidia-RNA-1_S3_L001_R1_001.fastp-trim.20220908.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-ctenidia-RNA-1_S3_L001_R1_001.fastp-trim.20220908.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3245
Sequences flagged as poor quality0
Sequence length32-151
%GC36

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC1494.591679506933744TruSeq Adapter, Index 12 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130.40061633281972264No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC130.40061633281972264TruSeq Adapter, Index 12 (100% over 50bp)
GATTTTGTTTTCTACATTTAACCTGGCTATGTCATTGTGCAGTCAGTATA120.3697996918335901No Hit
ATCAAAATGTCCACATGACCATGCCAGCCCCCCGGTACACCAATACTTGT120.3697996918335901No Hit
GTGTTTTTGAAATTTATCCTTAGGTGACAAAGTTTCAGACCGGACAGGAA110.3389830508474576No Hit
CCAGGAGCAACCTTCAGCAGATTTAAATGTGCAACATTTATCAGAGTGAT110.3389830508474576No Hit
ATTTTTTATAAACATCACAAAAGGATCATATTAATACAAAATAAATAAAA90.2773497688751926No Hit
CACCATGTCAGTTTAATCTCTTATATAAGAAGTATTTTACATATACATGT90.2773497688751926No Hit
CTGGTTTATAGTTAGATGTACTGGTATATAGTTAGGATATGAATATTGGT90.2773497688751926No Hit
GTGCTTTCATCAAACGAAACGATGCTTTGATCTGAACCATTGGCGGTCCA90.2773497688751926No Hit
TACTGAAGATCCATAGTACCACTGTACATCACTTGAAAATTAAATTAGAA90.2773497688751926No Hit
TGACATCGGGTGGTTTGAACAATCTTGTGTTTTGAAACAGTTTAATTCTT90.2773497688751926No Hit
TTTGACAGATGAGTCACAGGCTCGAACACAAGGAAGATAGGCAAGCTATC90.2773497688751926No Hit
TTTTTGTCCAGAAGATACAACGTAATGTTTAATTGGATATAGTTAACTTG90.2773497688751926No Hit
ATAGAGCGGTTTCTGCGACGATAATCAACAGCAATTCCAATGGTCATAGC80.2465331278890601No Hit
CCTCAATCTGCCCCACCATACCCTGGGATACATTGCGTGCCTCCAAAGCA80.2465331278890601No Hit
AAGGGCTAACAGACGATCAGTTAATCTAACCACTGTTTCCTGGGATCATA80.2465331278890601No Hit
TGAAAACAACAGTAATATGACCCCAATGTAAGAAGAAGCAATAAACTCCC80.2465331278890601No Hit
GGAAGAAGTCATAACAGCGTCAGAATTGCCAGCATGCGCACGGTGTTTTT80.2465331278890601No Hit
TTAAAGTACAAAACAGAATATGAGTTGTAACAATGGTGAAGCTGTACAAG80.2465331278890601No Hit
ATATATTCAATTTTAACATGAATATGTATAATTCATTAAAGCAAATAGTC80.2465331278890601No Hit
CGAGTTTTAGTTAACTTTATCAAAATACATATATTAATCTGATAAAGTTA80.2465331278890601No Hit
CCCGAATGGTTAAATGAAATAAATAAAATATCCTGATGTCCGTCTGCAAA80.2465331278890601No Hit
CATAAGTTCACTTCAATAATCTTTTATGTATAATAACCATTAAATTTAAT80.2465331278890601No Hit
AGGCAATGATGATAATGGTGAGTCCAAGATTCTGCTGACTGGCGATAAAT80.2465331278890601No Hit
CGGAGGTATCGGCCTTGTCGACTCTTCCTTCAACGCCTTTCCCTTTACCG80.2465331278890601No Hit
AAATTATACAAGCAAATTGTGGAAAAGTATGAAAAACATATTTAAATATA70.21571648690292758No Hit
CGTGCAGCTATTCGTGAGTGCAACATGATGCAGATTTAATATTGTATAGG70.21571648690292758No Hit
ATTGGACATTGAAGGAGAAGTCAAATAACGCATGGTTTTCATTTTTGCAT70.21571648690292758No Hit
TTAAGAACAGCAAATATACCTTAGTTTGACATCGGTTGCATTTATGTATG70.21571648690292758No Hit
GGGAGATAATACCAAACCCAGGAAGAATCAGAATGTATACTTCCGGGTGA70.21571648690292758No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.21571648690292758No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC70.21571648690292758No Hit
CAACTTCATGGTTGTATATCAATTTTCATAAAATTTTCATGATGCCTTCC70.21571648690292758No Hit
TCAATCTGAGATCTTCATCCAACAGTAAAACTGGATAGGCAATACATAAC70.21571648690292758No Hit
ATTTTTCTTTGGGTGAATCTCATCATACAAGTAATCTTCTGAAAGTTCCT70.21571648690292758No Hit
TTATTATTTACAATTTTACCCGTGAACGGGTTTCCAACATTTCTGTGAAA70.21571648690292758No Hit
GGGGCATGGTACTCTATACCACATTTAAAACAAAAATTAGTTTTACAAGT70.21571648690292758No Hit
CAGTTTGCTAAAGCCTTAATGTTTTTTATAACAATTTTCCTCAATCGCAC70.21571648690292758No Hit
GAGGATGTGCAACTGCAAGACATGCCAACACAAAAAAAATATATTATGCC70.21571648690292758No Hit
TTTCATTATGTTCCATGAATTGAATCAGTATTTCTTGATGTTCCATGAAT70.21571648690292758No Hit
GTTACTGATAGCGTAGTTGTTTCAGCCGGAGTGGTAGTAGCTGATGACGT60.18489984591679506No Hit
TTTCACTAGGCCTTGTTTTCATTGAGCCTCTTTTTCACTAGGCCTTGTTT60.18489984591679506No Hit
GGAACCAGTCTGTAAAATGTCCTAAATTGTGTTAATCTGATAACAGGACT60.18489984591679506No Hit
GTTTCACTGCACTGTATTTTACATTCCATATCACATACAAATGTTACATA60.18489984591679506No Hit
AAAAGTAACAAAACTGCCATAGGTAAAAGCCAAAACCTCATGTTATTTAT60.18489984591679506No Hit
GGCTTCTATTTGAATCTGCAGCTCAGCAAATGTCTTGTGCCAGTTCAGGA60.18489984591679506No Hit
GGGGCACCAGTCTTGATCTGACCAGTGCAGAGTCCAACGACAAGGTCAGC60.18489984591679506No Hit
CTCACAACACGTCGTCATGTTCTGTTGATCATTTGTTGCAAGTATCAACA60.18489984591679506No Hit
TTATAACAAAGGCATGCGCAGTCACAATTACATTATAGAGATGATCATCT60.18489984591679506No Hit
TTCTTCTTTCCAGATGCCTTCATGGCAGCTTTCGTTTTCTTGTCCTCAGC60.18489984591679506No Hit
GTCAGGGTGACATCTTGTTGCTTAACTGAAAGGAAGTTTTCCACTCCAAG60.18489984591679506No Hit
GTTTCACCAACATTTTAGTGTATAAAATTTTACATCTATTGAAAGCATCT60.18489984591679506No Hit
AAAGAACACCAGCATTCAAAATACTGTTTCAAATTATGTAATACTACAAG60.18489984591679506No Hit
CTGTGATGTTGTCCTTCTCCCAGGACTTTTTCACGTATTTAGCTTTGCAG60.18489984591679506No Hit
TTTTGCTTTAACTGCTGACATTCGAAACGAAATACATGTTTTACCTGTTG60.18489984591679506No Hit
ATTCGTCTCAACAATGGGTTAATCTGTGTGTCTCTGAGTCCACAGTCACC60.18489984591679506No Hit
GTGACTTTAATGTCGTTAATGTGTCTTGCAGCACCATACGCTGTCATGCC60.18489984591679506No Hit
TCTAGAACCAAGTCCTTAGAATAAAACCCTGCTATAAACGGAACCCCTAT60.18489984591679506No Hit
CAACTTTTAAATAATAATGATTTTGAAAAACAAAACAAAATATATTCATG60.18489984591679506No Hit
CTTGAACTCAAGAATGGATTTTGAAATTGATTTTGTATTCAATTAAACTC60.18489984591679506No Hit
ACAACATCACCTCTGTACAACATACAGCAGGCCATGTACTTGCCGTGACG60.18489984591679506No Hit
TGGGAACCTTGGCATATACAAAATATTTCCTTGGCAAAACTAAACTTGTT60.18489984591679506No Hit
ATTCTCACCCTGGGCTAAGCTCAACTGGTTGAGCAAGCCAGACTGATAAG60.18489984591679506No Hit
TTTTACATACCAGTAACTGTTTTTTAATGTGTTGAAATCTTGCAAGTTAA60.18489984591679506No Hit
GTCACGGTCAATATTTACTATATTATCTTGTCACGGTCAATATTTACCTG60.18489984591679506No Hit
GTCAGAGTCGCCGTGGTAGGTACCAGTGGGGTCGATGCCGTGCTCGTCGG60.18489984591679506No Hit
GCAAATCTCAGAACACTGCCCATACACTACACCAACATGATTTCTCTTTA60.18489984591679506No Hit
TTCACCTCTTTCGGAATGGCACGCCTCTATCACAGCAAATATATCGGTAG50.15408320493066258No Hit
CCCTTGTATGTTGAAGGACCTTGTCAGGACCATCTCATCTCCCACAGATA50.15408320493066258No Hit
CTCACGTTCCCGCTGCAACTGATTTTTCACATCAGTTAGCTCCTGCTGAA50.15408320493066258No Hit
CACTAACCTGTTCTTGACCTTCACACGTGCGCACTTAAATTTAACCACTG50.15408320493066258No Hit
CTTGCAAAGATTTAAATGAAAATGAACAAACAATTGGAAAATGATCAGAT50.15408320493066258No Hit
ACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC50.15408320493066258No Hit
GTAGATCGCAGATCTATACATTACCATATCATTATTATAATATTGAAGCA50.15408320493066258No Hit
CTTCTCTTCTGCTTTGGTCTCCTCTGCAGCACCTGCGGCTGGGGCTCCAC50.15408320493066258No Hit
ATATTACAAAATACAACAGATGTTGGTGTCAGGTCCGTGTGGAACTAACT50.15408320493066258No Hit
CAGCAACATTTTGGAGAGATCGCTGAAACTTCAGTTGGTTGACTCCGTGT50.15408320493066258No Hit
TTTTTTATCTATGTTCCAAACAGAAGAATTTATACTACTTCGTATTCCTT50.15408320493066258No Hit
TAACTTGATCAAAAACGTTTCTATGAGTATAAAGTATAATCTGAGATTAA50.15408320493066258No Hit
TAAGATGGCATCCTAAAATCACATAGCTAGCACATGTTTGTTGTAGAGAC50.15408320493066258No Hit
ATAGCTGGTTTTGCTTTAACAATGCCACCTTCCTTTGAACAGCATTCACT50.15408320493066258No Hit
TTTCCTTTCCTCTTCATCTTGTTCATCCTTCATTTTCTTCTCGGCATCTT50.15408320493066258No Hit
CTCCTTCCTCCATACCCTCTCCGACATACCAGTGGACAAATGCTCTCTTG50.15408320493066258No Hit
TTTTAACAAATTATATCAAAATGTTTGTCTTCAAACTCAAAATTGTTCAT50.15408320493066258No Hit
GGATCCTGGAAGATTAACTAGAACGCAAGTACACCCATCTCCACAAGTAA50.15408320493066258No Hit
AGACAAATAAGGATATCAAATTTTGATTTTCTCTTCGAATATTGCCAGTA50.15408320493066258No Hit
CTCTTCTGACTCATAGTGGCCATAGCACTTTTTGAAATTCAACAATATTT50.15408320493066258No Hit
GTTTTAATTGATCTTTGTAAAAACAACATTTACATTTAACCGCAAAACAC50.15408320493066258No Hit
CCCGCATCAAATTCCCATTAAACACGAAAATAACTAAAAAATACCCGACT50.15408320493066258No Hit
GCCAACCTTCGTCCAAACTCCATATTAGGCAACTGTTGCTTCATCTGCTG50.15408320493066258No Hit
GGTGAACATCACAGAGTTGCTGTCAGATGAGACATAAAATCAGCGAAAAA50.15408320493066258No Hit
GTCAGCATGTGTAATTCCCGGGAGCTTGAAAGAGTCTTTGAATCAGATAA50.15408320493066258No Hit
TAAAAAAGCAGCTATTATTCTCAATAAAAAAATAGTAGTACCAACCCACC50.15408320493066258No Hit
AATATGTATACCATATCAGTGCTCATTTGATCAAACTTAGTAATTTATAA50.15408320493066258No Hit
GCCCAGCTGACTTCAATGATCTATCTAAAGCTGTCTTTGTAATTTAAATT50.15408320493066258No Hit
GTCCATGGTAATGTCTTGCTTTAATCCTTTGTTACCATGGTTGCACCATA50.15408320493066258No Hit
AGGTCGACTCATAATGGTATAACATGTTTGAGAACATATAGGTAATCTCA50.15408320493066258No Hit
CTGCACAGCAGAACAATGTTTCACACATGATGTATGTATTTGATCATGTG40.12326656394453005No Hit
GTTCTGCACGTTCAACATCTGTTCGTCGACCTCCTTCATGGACATACGTC40.12326656394453005No Hit
CAATATTGTACTTCAATGGACTTTAAAAAAATCATTTTCAATTTTTTTTT40.12326656394453005No Hit
ACTACAGCTCCATTTTTCTACGGGAAGAGTATGTCAATGTTATATACATG40.12326656394453005No Hit
AGATGATATCCATGTAGTGGTGATCAGCGTGTTACAATTTAATTAACATC40.12326656394453005No Hit
TTTGATACTAATTTGATAAAACACAAGATATGGTACCCTTTCTCTGGCAG40.12326656394453005No Hit
AGAAGAAAATATTAAACTTGTACTTGGTTAAAAAAAAGATACAATAACTG40.12326656394453005No Hit
CCCCTCACCACCCAGGGCAATAATTCTCTTTAACTCCTTGTCCACTTGAT40.12326656394453005No Hit
TGATTCTGCACACAGTCAAAGGTATTTATTGCTGTATTTCTTTTCATGCC40.12326656394453005No Hit
GTTATGTCTCAACACAAGGGAAATGTTTGGGGTTTTTTTGAATGGGGTTT40.12326656394453005No Hit
GTATTCTTGGTACTCTCATCAAGCTGGTCCAAGTAGTTGAACATGAAATC40.12326656394453005No Hit
ACAAAATAAAAATCTTGATATATTATTAGTTTTAAAACAATGTCCAATGT40.12326656394453005No Hit
GTTAAGTCAATAACTATCTTTTACAAGTGTTTAAAACTCATCATTGAGCA40.12326656394453005No Hit
CTTGCCTTGAGTGAGGGTTTCTTCGTCAAAGTGCCTAGGATTAAATTGGC40.12326656394453005No Hit
TTTCAACCATGTTTATCAGGCAACTTTAACCTAACACAAATACATTTAAG40.12326656394453005No Hit
TACATATATGCATATGAGACTTCAGAAATTAAACATTTCTTTACAAAGAA40.12326656394453005No Hit
TAGGTTATGTCACTTACACTTCATACCATTTCCTGCATGATTACATTGTA40.12326656394453005No Hit
CCGCCGTCCCGTCTGCGTCCGTAGCTGTTACAGTGCCGACAGTGGTTGCT40.12326656394453005No Hit
GGCTACACAAAACTTTCCCTCAATTCAATTTTAAAAACATTAGTGCCCAA40.12326656394453005No Hit
CACGGGCCTCGGAGAACTCACCCTCCTCCATACCCTCTCCGACGTACCAA40.12326656394453005No Hit
TATTTGGTTGTAAAATCAATTCATAATACACTTTAACGTTAATAAAGATT40.12326656394453005No Hit
AAGATCTTGGGGACGGACGGATGAAGCGGCGACTATATGCTTCCCCCCTT40.12326656394453005No Hit
TAAAATAAGCTCGCCTATCAATATTTATGCCAACCGTAAATATGTGATGA40.12326656394453005No Hit
GTTCGGATTTCTCGACTCATCCGTGGTCAATATTCCATACAGAAGGGCAG40.12326656394453005No Hit
TACATGTTGTGTTAAGCTGTATATATTTTATGTTTGACAGTTATGCAATG40.12326656394453005No Hit
CCCTAGTAAATCCTTGTCCACATCTTCATCATCGTCAATACCTCGGAGAT40.12326656394453005No Hit
CTAGACCTAAAATTATCCTTTAAATACTGAGTGCCAAGCGAGGAAGCTTC40.12326656394453005No Hit
CGAGAATGTGTCGTAGGCATCAGGATCAGATGCGTAAATTCCACAGTGGC40.12326656394453005No Hit
CTTTTGTCTTGACCACTCACAACCTCCGGCATGCCTCTTAATTTTTCATC40.12326656394453005No Hit
CGTAATTGTCCATGATTTGCAATAGATACATCCCTCCTGAGCAGGTCAAT40.12326656394453005No Hit
GTCCAGTTTAAGGGTCATATCCTCCAGAATTTTGCGTTCTGGTCCAAGGA40.12326656394453005No Hit
TTCCAACACGTTATTTGACAACCAAAATTACTAGCGGAGGAATCGTAAAC40.12326656394453005No Hit
AAGACTGTTATTTTACCAATTTGTGTTACTGTTCCTTTTTACAAATTGAC40.12326656394453005No Hit
GATATGTCCACTTTAAGTCTTTATTGATTAAATTGTTTAGCTGTATGTCA40.12326656394453005No Hit
TTGTCCTGTCTGCTGGAAGGCTTTGAAGTGTAACTCAGTTCTGCGACACA40.12326656394453005No Hit

[OK]Adapter Content

Adapter graph