This is cutadapt 2.10 with Python 3.6.7 Command line parameters: -j 1 -a AGATCGGAAGAGC -u 10 -u -10 -q 20 -m 30 -O 3 -o fastq/zr1394_9_s456.fastq.gz zr1394_9_s456.fastq.gz Processing reads on 1 core in single-end mode ... Finished in 1489.94 s (19 us/read; 3.15 M reads/minute). === Summary === Total reads processed: 78,146,508 Reads with adapters: 1,168,414 (1.5%) Reads written (passing filters): 65,129,633 (83.3%) Total basepairs processed: 3,934,245,065 bp Quality-trimmed: 20,323,766 bp (0.5%) Total written (filtered): 2,004,986,379 bp (51.0%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 1168414 times No. of allowed errors: 0-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 27.5% C: 1.0% G: 28.3% T: 43.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 802300 1221039.2 0 802300 4 324810 305259.8 0 324810 5 13842 76314.9 0 13842 6 12659 19078.7 0 12659 7 4343 4769.7 0 4343 8 928 1192.4 0 928 9 2997 298.1 0 773 2224 10 4517 74.5 1 89 4428 11 1729 18.6 1 22 1707 12 261 4.7 1 0 261 13 21 1.2 1 0 21 14 3 1.2 1 0 3 16 1 1.2 1 0 1 17 1 1.2 1 0 1 18 2 1.2 1 0 2