This is cutadapt 2.10 with Python 3.6.7 Command line parameters: -j 1 -a AGATCGGAAGAGC -u 10 -u -10 -q 20 -m 30 -O 3 -o fastq/zr1394_8_s456.fastq.gz zr1394_8_s456.fastq.gz Processing reads on 1 core in single-end mode ... Finished in 777.20 s (20 us/read; 3.02 M reads/minute). === Summary === Total reads processed: 39,136,514 Reads with adapters: 602,200 (1.5%) Reads written (passing filters): 32,285,894 (82.5%) Total basepairs processed: 1,968,545,164 bp Quality-trimmed: 10,767,486 bp (0.5%) Total written (filtered): 993,598,628 bp (50.5%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 602200 times No. of allowed errors: 0-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 26.2% C: 1.1% G: 30.9% T: 41.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 413954 611508.0 0 413954 4 159591 152877.0 0 159591 5 9229 38219.3 0 9229 6 8708 9554.8 0 8708 7 2976 2388.7 0 2976 8 712 597.2 0 712 9 1939 149.3 0 635 1304 10 3493 37.3 1 98 3395 11 1366 9.3 1 12 1354 12 191 2.3 1 1 190 13 31 0.6 1 0 31 14 1 0.6 1 0 1 15 1 0.6 1 0 1 16 1 0.6 1 0 1 17 2 0.6 1 0 2 18 4 0.6 1 0 4 19 1 0.6 1 0 1