This is cutadapt 2.10 with Python 3.6.7 Command line parameters: -j 1 -a AGATCGGAAGAGC -u 10 -u -10 -q 20 -m 30 -O 3 -o fastq/zr1394_6_s456.fastq.gz zr1394_6_s456.fastq.gz Processing reads on 1 core in single-end mode ... Finished in 939.64 s (20 us/read; 3.00 M reads/minute). === Summary === Total reads processed: 46,944,411 Reads with adapters: 717,062 (1.5%) Reads written (passing filters): 39,200,355 (83.5%) Total basepairs processed: 2,363,416,290 bp Quality-trimmed: 11,998,579 bp (0.5%) Total written (filtered): 1,206,804,534 bp (51.1%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 717062 times No. of allowed errors: 0-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 27.3% C: 1.1% G: 28.5% T: 43.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 492535 733506.4 0 492535 4 199329 183376.6 0 199329 5 8411 45844.2 0 8411 6 7555 11461.0 0 7555 7 2752 2865.3 0 2752 8 720 716.3 0 720 9 1651 179.1 0 533 1118 10 2707 44.8 1 99 2608 11 1115 11.2 1 40 1075 12 233 2.8 1 2 231 13 29 0.7 1 0 29 14 6 0.7 1 0 6 15 11 0.7 1 0 11 16 7 0.7 1 0 7 18 1 0.7 1 0 1