This is cutadapt 2.10 with Python 3.6.7 Command line parameters: -j 1 -a AGATCGGAAGAGC -u 10 -u -10 -q 20 -m 30 -O 3 -o fastq/zr1394_5_s456.fastq.gz zr1394_5_s456.fastq.gz Processing reads on 1 core in single-end mode ... Finished in 969.26 s (20 us/read; 2.97 M reads/minute). === Summary === Total reads processed: 48,050,056 Reads with adapters: 778,048 (1.6%) Reads written (passing filters): 39,554,319 (82.3%) Total basepairs processed: 2,414,803,052 bp Quality-trimmed: 12,441,575 bp (0.5%) Total written (filtered): 1,217,401,123 bp (50.4%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 778048 times No. of allowed errors: 0-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 26.7% C: 1.0% G: 30.2% T: 42.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 538539 750782.1 0 538539 4 209406 187695.5 0 209406 5 10152 46923.9 0 10152 6 8700 11731.0 0 8700 7 3441 2932.7 0 3441 8 947 733.2 0 947 9 2011 183.3 0 628 1383 10 3039 45.8 1 102 2937 11 1467 11.5 1 31 1436 12 263 2.9 1 0 263 13 60 0.7 1 0 60 14 8 0.7 1 0 8 15 3 0.7 1 0 3 16 5 0.7 1 0 5 17 2 0.7 1 0 2 18 1 0.7 1 0 1 19 3 0.7 1 0 3 20 1 0.7 1 0 1