This is cutadapt 2.10 with Python 3.6.7 Command line parameters: -j 1 -a AGATCGGAAGAGC -u 10 -u -10 -q 20 -m 30 -O 3 -o fastq/zr1394_4_s456.fastq.gz zr1394_4_s456.fastq.gz Processing reads on 1 core in single-end mode ... Finished in 907.89 s (20 us/read; 3.00 M reads/minute). === Summary === Total reads processed: 45,411,925 Reads with adapters: 688,413 (1.5%) Reads written (passing filters): 37,491,087 (82.6%) Total basepairs processed: 2,284,287,588 bp Quality-trimmed: 12,380,561 bp (0.5%) Total written (filtered): 1,153,781,294 bp (50.5%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 688413 times No. of allowed errors: 0-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 26.3% C: 1.1% G: 30.2% T: 42.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 471878 709561.3 0 471878 4 183898 177390.3 0 183898 5 10405 44347.6 0 10405 6 9784 11086.9 0 9784 7 3756 2771.7 0 3756 8 851 692.9 0 851 9 2229 173.2 0 781 1448 10 3702 43.3 1 117 3585 11 1633 10.8 1 24 1609 12 222 2.7 1 0 222 13 41 0.7 1 0 41 14 3 0.7 1 0 3 15 4 0.7 1 0 4 16 2 0.7 1 0 2 17 2 0.7 1 0 2 18 3 0.7 1 0 3