This is cutadapt 2.10 with Python 3.6.7 Command line parameters: -j 1 -a AGATCGGAAGAGC -u 10 -u -10 -q 20 -m 30 -O 3 -o fastq/zr1394_3_s456.fastq.gz zr1394_3_s456.fastq.gz Processing reads on 1 core in single-end mode ... Finished in 975.33 s (20 us/read; 3.01 M reads/minute). === Summary === Total reads processed: 48,926,620 Reads with adapters: 732,427 (1.5%) Reads written (passing filters): 40,468,296 (82.7%) Total basepairs processed: 2,461,374,827 bp Quality-trimmed: 13,361,018 bp (0.5%) Total written (filtered): 1,245,550,837 bp (50.6%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 732427 times No. of allowed errors: 0-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 26.7% C: 1.1% G: 29.5% T: 42.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 502768 764478.4 0 502768 4 197087 191119.6 0 197087 5 10387 47779.9 0 10387 6 9640 11945.0 0 9640 7 3619 2986.2 0 3619 8 797 746.6 0 797 9 2130 186.6 0 674 1456 10 4235 46.7 1 107 4128 11 1447 11.7 1 25 1422 12 238 2.9 1 0 238 13 65 0.7 1 0 65 14 1 0.7 1 0 1 15 4 0.7 1 0 4 16 6 0.7 1 0 6 17 1 0.7 1 0 1 18 1 0.7 1 0 1 20 1 0.7 1 0 1