This is cutadapt 2.10 with Python 3.6.7 Command line parameters: -j 1 -a AGATCGGAAGAGC -u 10 -u -10 -q 20 -m 30 -O 3 -o fastq/zr1394_2_s456.fastq.gz zr1394_2_s456.fastq.gz Processing reads on 1 core in single-end mode ... Finished in 932.22 s (20 us/read; 3.00 M reads/minute). === Summary === Total reads processed: 46,666,438 Reads with adapters: 751,050 (1.6%) Reads written (passing filters): 38,400,341 (82.3%) Total basepairs processed: 2,346,424,714 bp Quality-trimmed: 12,720,314 bp (0.5%) Total written (filtered): 1,181,771,081 bp (50.4%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 751050 times No. of allowed errors: 0-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 26.3% C: 1.0% G: 30.6% T: 42.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 517285 729163.1 0 517285 4 199697 182290.8 0 199697 5 10985 45572.7 0 10985 6 9675 11393.2 0 9675 7 3640 2848.3 0 3640 8 888 712.1 0 888 9 2284 178.0 0 793 1491 10 4571 44.5 1 102 4469 11 1712 11.1 1 20 1692 12 256 2.8 1 1 255 13 34 0.7 1 0 34 14 3 0.7 1 0 3 15 5 0.7 1 0 5 16 6 0.7 1 0 6 17 1 0.7 1 0 1 18 3 0.7 1 0 3 19 5 0.7 1 0 5