This is cutadapt 2.10 with Python 3.6.7 Command line parameters: -j 1 -a AGATCGGAAGAGC -u 10 -u -10 -q 20 -m 30 -O 3 -o fastq/zr1394_1_s456.fastq.gz zr1394_1_s456.fastq.gz Processing reads on 1 core in single-end mode ... Finished in 957.47 s (20 us/read; 2.97 M reads/minute). === Summary === Total reads processed: 47,399,413 Reads with adapters: 729,179 (1.5%) Reads written (passing filters): 39,088,361 (82.5%) Total basepairs processed: 2,382,626,805 bp Quality-trimmed: 12,158,681 bp (0.5%) Total written (filtered): 1,203,018,264 bp (50.5%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 729179 times No. of allowed errors: 0-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 26.6% C: 1.1% G: 29.9% T: 42.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 500862 740615.8 0 500862 4 194831 185154.0 0 194831 5 10787 46288.5 0 10787 6 10261 11572.1 0 10261 7 3417 2893.0 0 3417 8 1005 723.3 0 1005 9 2272 180.8 0 802 1470 10 3717 45.2 1 112 3605 11 1736 11.3 1 35 1701 12 223 2.8 1 1 222 13 46 0.7 1 0 46 14 6 0.7 1 0 6 15 3 0.7 1 0 3 16 5 0.7 1 0 5 17 5 0.7 1 0 5 18 3 0.7 1 0 3