This is cutadapt 2.10 with Python 3.6.7 Command line parameters: -j 1 -a AGATCGGAAGAGC -u 10 -u -10 -q 20 -m 30 -O 3 -o fastq/zr1394_17_s456.fastq.gz zr1394_17_s456.fastq.gz Processing reads on 1 core in single-end mode ... Finished in 858.98 s (20 us/read; 2.98 M reads/minute). === Summary === Total reads processed: 42,730,485 Reads with adapters: 677,848 (1.6%) Reads written (passing filters): 34,419,823 (80.6%) Total basepairs processed: 2,143,526,203 bp Quality-trimmed: 12,003,101 bp (0.6%) Total written (filtered): 1,058,757,927 bp (49.4%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 677848 times No. of allowed errors: 0-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 26.0% C: 1.2% G: 31.4% T: 41.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 458457 667663.8 0 458457 4 178931 166916.0 0 178931 5 12041 41729.0 0 12041 6 11728 10432.2 0 11728 7 4518 2608.1 0 4518 8 1115 652.0 0 1115 9 2777 163.0 0 1056 1721 10 5393 40.8 1 200 5193 11 2582 10.2 1 26 2556 12 259 2.5 1 1 258 13 36 0.6 1 0 36 14 2 0.6 1 0 2 15 3 0.6 1 0 3 16 3 0.6 1 0 3 17 3 0.6 1 0 3