This is cutadapt 2.10 with Python 3.6.7 Command line parameters: -j 1 -a AGATCGGAAGAGC -u 10 -u -10 -q 20 -m 30 -O 3 -o fastq/zr1394_16_s456.fastq.gz zr1394_16_s456.fastq.gz Processing reads on 1 core in single-end mode ... Finished in 1428.04 s (19 us/read; 3.17 M reads/minute). === Summary === Total reads processed: 75,450,315 Reads with adapters: 1,102,741 (1.5%) Reads written (passing filters): 61,542,150 (81.6%) Total basepairs processed: 3,791,761,278 bp Quality-trimmed: 22,003,997 bp (0.6%) Total written (filtered): 1,893,380,610 bp (49.9%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 1102741 times No. of allowed errors: 0-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 27.0% C: 1.2% G: 29.6% T: 42.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 749240 1178911.2 0 749240 4 292862 294727.8 0 292862 5 19171 73681.9 0 19171 6 18827 18420.5 0 18827 7 7913 4605.1 0 7913 8 1300 1151.3 0 1300 9 3554 287.8 0 1126 2428 10 6491 72.0 1 131 6360 11 2960 18.0 1 32 2928 12 333 4.5 1 0 333 13 79 1.1 1 0 79 14 4 1.1 1 0 4 15 2 1.1 1 0 2 16 1 1.1 1 0 1 17 2 1.1 1 0 2 18 1 1.1 1 0 1 19 1 1.1 1 0 1