This is cutadapt 2.10 with Python 3.6.7 Command line parameters: -j 1 -a AGATCGGAAGAGC -u 10 -u -10 -q 20 -m 30 -O 3 -o fastq/zr1394_15_s456.fastq.gz zr1394_15_s456.fastq.gz Processing reads on 1 core in single-end mode ... Finished in 1330.07 s (19 us/read; 3.11 M reads/minute). === Summary === Total reads processed: 68,857,992 Reads with adapters: 1,023,438 (1.5%) Reads written (passing filters): 56,071,211 (81.4%) Total basepairs processed: 3,459,545,810 bp Quality-trimmed: 20,919,625 bp (0.6%) Total written (filtered): 1,725,046,941 bp (49.9%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 1023438 times No. of allowed errors: 0-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 26.4% C: 1.2% G: 30.4% T: 42.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 695385 1075906.1 0 695385 4 269730 268976.5 0 269730 5 17743 67244.1 0 17743 6 18187 16811.0 0 18187 7 5676 4202.8 0 5676 8 1327 1050.7 0 1327 9 3841 262.7 0 1356 2485 10 7578 65.7 1 132 7446 11 3585 16.4 1 37 3548 12 337 4.1 1 1 336 13 42 1.0 1 0 42 14 1 1.0 1 0 1 15 3 1.0 1 0 3 16 1 1.0 1 0 1 17 1 1.0 1 0 1 19 1 1.0 1 0 1