This is cutadapt 2.10 with Python 3.6.7 Command line parameters: -j 1 -a AGATCGGAAGAGC -u 10 -u -10 -q 20 -m 30 -O 3 -o fastq/zr1394_14_s456.fastq.gz zr1394_14_s456.fastq.gz Processing reads on 1 core in single-end mode ... Finished in 1647.06 s (19 us/read; 3.23 M reads/minute). === Summary === Total reads processed: 88,771,906 Reads with adapters: 1,246,050 (1.4%) Reads written (passing filters): 73,216,298 (82.5%) Total basepairs processed: 4,466,488,793 bp Quality-trimmed: 26,657,368 bp (0.6%) Total written (filtered): 2,253,269,802 bp (50.4%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 1246050 times No. of allowed errors: 0-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 27.4% C: 1.2% G: 28.2% T: 43.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 846829 1387061.0 0 846829 4 337284 346765.3 0 337284 5 19858 86691.3 0 19858 6 18993 21672.8 0 18993 7 7095 5418.2 0 7095 8 1353 1354.6 0 1353 9 4141 338.6 0 1289 2852 10 6982 84.7 1 124 6858 11 3055 21.2 1 24 3031 12 377 5.3 1 0 377 13 80 1.3 1 0 80 15 1 1.3 1 0 1 17 2 1.3 1 0 2