This is cutadapt 2.10 with Python 3.6.7 Command line parameters: -j 1 -a AGATCGGAAGAGC -u 10 -u -10 -q 20 -m 30 -O 3 -o fastq/zr1394_13_s456.fastq.gz zr1394_13_s456.fastq.gz Processing reads on 1 core in single-end mode ... Finished in 1259.63 s (19 us/read; 3.10 M reads/minute). === Summary === Total reads processed: 65,081,682 Reads with adapters: 971,919 (1.5%) Reads written (passing filters): 53,521,280 (82.2%) Total basepairs processed: 3,272,837,182 bp Quality-trimmed: 17,942,807 bp (0.5%) Total written (filtered): 1,646,886,497 bp (50.3%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 971919 times No. of allowed errors: 0-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 26.5% C: 1.1% G: 30.4% T: 42.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 659703 1016901.3 0 659703 4 259444 254225.3 0 259444 5 16440 63556.3 0 16440 6 15696 15889.1 0 15696 7 5812 3972.3 0 5812 8 1123 993.1 0 1123 9 3445 248.3 0 1120 2325 10 6751 62.1 1 119 6632 11 3138 15.5 1 33 3105 12 294 3.9 1 0 294 13 68 1.0 1 0 68 14 1 1.0 1 0 1 16 3 1.0 1 0 3 17 1 1.0 1 0 1