This is cutadapt 2.10 with Python 3.6.7 Command line parameters: -j 1 -a AGATCGGAAGAGC -u 10 -u -10 -q 20 -m 30 -O 3 -o fastq/zr1394_12_s456.fastq.gz zr1394_12_s456.fastq.gz Processing reads on 1 core in single-end mode ... Finished in 1182.59 s (20 us/read; 3.05 M reads/minute). === Summary === Total reads processed: 60,153,246 Reads with adapters: 932,013 (1.5%) Reads written (passing filters): 49,469,701 (82.2%) Total basepairs processed: 3,025,392,455 bp Quality-trimmed: 18,372,170 bp (0.6%) Total written (filtered): 1,522,543,617 bp (50.3%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 932013 times No. of allowed errors: 0-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 27.2% C: 1.0% G: 28.8% T: 42.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 638265 939894.5 0 638265 4 258072 234973.6 0 258072 5 12142 58743.4 0 12142 6 10661 14685.9 0 10661 7 3819 3671.5 0 3819 8 828 917.9 0 828 9 2392 229.5 0 643 1749 10 3871 57.4 1 92 3779 11 1693 14.3 1 18 1675 12 236 3.6 1 0 236 13 28 0.9 1 0 28 14 2 0.9 1 0 2 15 2 0.9 1 0 2 16 1 0.9 1 0 1 20 1 0.9 1 0 1