This is cutadapt 2.10 with Python 3.6.7 Command line parameters: -j 1 -a AGATCGGAAGAGC -u 10 -q 20 -m 36 -O 3 -o fastq/zr1394_9_s456.fastq.gz zr1394_9_s456.fastq.gz Processing reads on 1 core in single-end mode ... Finished in 1845.95 s (24 us/read; 2.54 M reads/minute). === Summary === Total reads processed: 78,146,508 Reads with adapters: 413,098 (0.5%) Reads written (passing filters): 76,352,943 (97.7%) Total basepairs processed: 3,934,245,065 bp Quality-trimmed: 3,577,475 bp (0.1%) Total written (filtered): 3,096,306,876 bp (78.7%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 413098 times No. of allowed errors: 0-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 26.8% C: 1.4% G: 33.9% T: 37.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 325611 1221039.2 0 325611 4 73104 305259.8 0 73104 5 8029 76314.9 0 8029 6 742 19078.7 0 742 7 2218 4769.7 0 2218 8 799 1192.4 0 799 9 472 298.1 0 40 432 10 853 74.5 1 8 845 11 285 18.6 1 24 261 12 141 4.7 1 0 141 13 49 1.2 1 0 49 14 49 1.2 1 0 49 15 68 1.2 1 0 68 16 149 1.2 1 0 149 17 328 1.2 1 0 328 18 179 1.2 1 0 179 19 11 1.2 1 0 11 20 1 1.2 1 0 1 21 2 1.2 1 0 2 23 1 1.2 1 0 1 24 3 1.2 1 0 3 26 1 1.2 1 0 1 27 1 1.2 1 0 1 28 2 1.2 1 0 2