This is cutadapt 2.10 with Python 3.6.7 Command line parameters: -j 1 -a AGATCGGAAGAGC -u 10 -q 20 -m 36 -O 3 -o fastq/zr1394_8_s456.fastq.gz zr1394_8_s456.fastq.gz Processing reads on 1 core in single-end mode ... Finished in 992.07 s (25 us/read; 2.37 M reads/minute). === Summary === Total reads processed: 39,136,514 Reads with adapters: 230,609 (0.6%) Reads written (passing filters): 38,164,529 (97.5%) Total basepairs processed: 1,968,545,164 bp Quality-trimmed: 1,949,079 bp (0.1%) Total written (filtered): 1,546,696,823 bp (78.6%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 230609 times No. of allowed errors: 0-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 26.3% C: 1.7% G: 36.7% T: 35.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 184710 611508.0 0 184710 4 36397 152877.0 0 36397 5 4812 38219.3 0 4812 6 469 9554.8 0 469 7 1447 2388.7 0 1447 8 486 597.2 0 486 9 271 149.3 0 21 250 10 614 37.3 1 13 601 11 249 9.3 1 26 223 12 135 2.3 1 0 135 13 60 0.6 1 0 60 14 47 0.6 1 0 47 15 77 0.6 1 0 77 16 168 0.6 1 0 168 17 390 0.6 1 0 390 18 222 0.6 1 0 222 19 31 0.6 1 0 31 20 4 0.6 1 0 4 21 4 0.6 1 0 4 22 3 0.6 1 0 3 23 3 0.6 1 0 3 24 2 0.6 1 0 2 25 1 0.6 1 0 1 27 2 0.6 1 0 2 28 4 0.6 1 0 4 29 1 0.6 1 0 1