This is cutadapt 2.10 with Python 3.6.7 Command line parameters: -j 1 -a AGATCGGAAGAGC -u 10 -q 20 -m 36 -O 3 -o fastq/zr1394_7_s456.fastq.gz zr1394_7_s456.fastq.gz Processing reads on 1 core in single-end mode ... Finished in 1263.60 s (25 us/read; 2.39 M reads/minute). === Summary === Total reads processed: 50,399,353 Reads with adapters: 296,848 (0.6%) Reads written (passing filters): 48,970,555 (97.2%) Total basepairs processed: 2,532,578,583 bp Quality-trimmed: 2,586,471 bp (0.1%) Total written (filtered): 1,984,371,775 bp (78.4%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 296848 times No. of allowed errors: 0-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 27.9% C: 2.3% G: 32.9% T: 36.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 233197 787489.9 0 233197 4 47943 196872.5 0 47943 5 6969 49218.1 0 6969 6 678 12304.5 0 678 7 1960 3076.1 0 1960 8 1003 769.0 0 1003 9 472 192.3 0 59 413 10 855 48.1 1 18 837 11 462 12.0 1 57 405 12 300 3.0 1 4 296 13 128 0.8 1 0 128 14 117 0.8 1 0 117 15 211 0.8 1 0 211 16 494 0.8 1 0 494 17 1163 0.8 1 0 1163 18 750 0.8 1 0 750 19 96 0.8 1 0 96 20 9 0.8 1 0 9 21 10 0.8 1 0 10 22 4 0.8 1 0 4 23 6 0.8 1 0 6 24 7 0.8 1 0 7 26 3 0.8 1 0 3 27 6 0.8 1 0 6 28 2 0.8 1 0 2 29 1 0.8 1 0 1 30 2 0.8 1 0 2