This is cutadapt 2.10 with Python 3.6.7 Command line parameters: -j 1 -a AGATCGGAAGAGC -u 10 -q 20 -m 36 -O 3 -o fastq/zr1394_5_s456.fastq.gz zr1394_5_s456.fastq.gz Processing reads on 1 core in single-end mode ... Finished in 1196.70 s (25 us/read; 2.41 M reads/minute). === Summary === Total reads processed: 48,050,056 Reads with adapters: 319,920 (0.7%) Reads written (passing filters): 46,668,674 (97.1%) Total basepairs processed: 2,414,803,052 bp Quality-trimmed: 2,378,847 bp (0.1%) Total written (filtered): 1,891,513,778 bp (78.3%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 319920 times No. of allowed errors: 0-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 27.0% C: 2.0% G: 35.5% T: 35.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 253596 750782.1 0 253596 4 51677 187695.5 0 51677 5 6399 46923.9 0 6399 6 650 11731.0 0 650 7 1756 2932.7 0 1756 8 1012 733.2 0 1012 9 475 183.3 0 59 416 10 803 45.8 1 16 787 11 402 11.5 1 50 352 12 289 2.9 1 1 288 13 126 0.7 1 0 126 14 130 0.7 1 0 130 15 212 0.7 1 0 212 16 509 0.7 1 0 509 17 1079 0.7 1 0 1079 18 675 0.7 1 0 675 19 71 0.7 1 0 71 20 15 0.7 1 0 15 21 5 0.7 1 0 5 22 8 0.7 1 0 8 23 8 0.7 1 0 8 24 7 0.7 1 0 7 25 5 0.7 1 0 5 26 5 0.7 1 0 5 27 2 0.7 1 0 2 28 1 0.7 1 0 1 29 2 0.7 1 0 2 30 1 0.7 1 0 1