This is cutadapt 2.10 with Python 3.6.7 Command line parameters: -j 1 -a AGATCGGAAGAGC -u 10 -q 20 -m 36 -O 3 -o fastq/zr1394_4_s456.fastq.gz zr1394_4_s456.fastq.gz Processing reads on 1 core in single-end mode ... Finished in 1140.25 s (25 us/read; 2.39 M reads/minute). === Summary === Total reads processed: 45,411,925 Reads with adapters: 259,693 (0.6%) Reads written (passing filters): 44,296,827 (97.5%) Total basepairs processed: 2,284,287,588 bp Quality-trimmed: 2,300,949 bp (0.1%) Total written (filtered): 1,795,271,497 bp (78.6%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 259693 times No. of allowed errors: 0-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 25.9% C: 1.6% G: 36.4% T: 36.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 205617 709561.3 0 205617 4 42458 177390.3 0 42458 5 5507 44347.6 0 5507 6 613 11086.9 0 613 7 1579 2771.7 0 1579 8 658 692.9 0 658 9 360 173.2 0 34 326 10 695 43.3 1 10 685 11 288 10.8 1 42 246 12 175 2.7 1 3 172 13 84 0.7 1 0 84 14 69 0.7 1 0 69 15 133 0.7 1 0 133 16 280 0.7 1 0 280 17 691 0.7 1 0 691 18 394 0.7 1 0 394 19 54 0.7 1 0 54 20 9 0.7 1 0 9 21 5 0.7 1 0 5 22 7 0.7 1 0 7 23 3 0.7 1 0 3 24 2 0.7 1 0 2 25 4 0.7 1 0 4 26 4 0.7 1 0 4 27 2 0.7 1 0 2 28 2 0.7 1 0 2