This is cutadapt 2.10 with Python 3.6.7 Command line parameters: -j 1 -a AGATCGGAAGAGC -u 10 -q 20 -m 36 -O 3 -o fastq/zr1394_18_s456.fastq.gz zr1394_18_s456.fastq.gz Processing reads on 1 core in single-end mode ... Finished in 1382.13 s (25 us/read; 2.41 M reads/minute). === Summary === Total reads processed: 55,559,681 Reads with adapters: 310,449 (0.6%) Reads written (passing filters): 54,358,757 (97.8%) Total basepairs processed: 2,797,597,463 bp Quality-trimmed: 2,612,426 bp (0.1%) Total written (filtered): 2,203,688,495 bp (78.8%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 310449 times No. of allowed errors: 0-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 27.3% C: 1.5% G: 34.4% T: 36.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 244906 868120.0 0 244906 4 53796 217030.0 0 53796 5 6115 54257.5 0 6115 6 628 13564.4 0 628 7 1871 3391.1 0 1871 8 655 847.8 0 655 9 371 211.9 0 27 344 10 864 53.0 1 10 854 11 285 13.2 1 31 254 12 142 3.3 1 1 141 13 68 0.8 1 0 68 14 63 0.8 1 0 63 15 72 0.8 1 0 72 16 165 0.8 1 0 165 17 245 0.8 1 0 245 18 166 0.8 1 0 166 19 17 0.8 1 0 17 20 4 0.8 1 0 4 21 3 0.8 1 0 3 22 1 0.8 1 0 1 23 2 0.8 1 0 2 24 3 0.8 1 0 3 26 7 0.8 1 0 7