This is cutadapt 2.10 with Python 3.6.7 Command line parameters: -j 1 -a AGATCGGAAGAGC -u 10 -q 20 -m 36 -O 3 -o fastq/zr1394_16_s456.fastq.gz zr1394_16_s456.fastq.gz Processing reads on 1 core in single-end mode ... Finished in 1819.63 s (24 us/read; 2.49 M reads/minute). === Summary === Total reads processed: 75,450,315 Reads with adapters: 402,705 (0.5%) Reads written (passing filters): 73,398,348 (97.3%) Total basepairs processed: 3,791,761,278 bp Quality-trimmed: 4,187,359 bp (0.1%) Total written (filtered): 2,972,900,111 bp (78.4%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 402705 times No. of allowed errors: 0-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 27.8% C: 2.0% G: 33.0% T: 37.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 317730 1178911.2 0 317730 4 66025 294727.8 0 66025 5 10401 73681.9 0 10401 6 961 18420.5 0 961 7 2933 4605.1 0 2933 8 1094 1151.3 0 1094 9 601 287.8 0 66 535 10 1198 72.0 1 9 1189 11 374 18.0 1 39 335 12 183 4.5 1 1 182 13 69 1.1 1 0 69 14 71 1.1 1 0 71 15 93 1.1 1 0 93 16 222 1.1 1 0 222 17 427 1.1 1 0 427 18 273 1.1 1 0 273 19 26 1.1 1 0 26 20 5 1.1 1 0 5 21 4 1.1 1 0 4 22 2 1.1 1 0 2 23 1 1.1 1 0 1 24 4 1.1 1 0 4 25 2 1.1 1 0 2 26 1 1.1 1 0 1 27 3 1.1 1 0 3 28 1 1.1 1 0 1 29 1 1.1 1 0 1