This is cutadapt 2.10 with Python 3.6.7 Command line parameters: -j 1 -a AGATCGGAAGAGC -u 10 -q 20 -m 36 -O 3 -o fastq/zr1394_15_s456.fastq.gz zr1394_15_s456.fastq.gz Processing reads on 1 core in single-end mode ... Finished in 1661.58 s (24 us/read; 2.49 M reads/minute). === Summary === Total reads processed: 68,857,992 Reads with adapters: 384,802 (0.6%) Reads written (passing filters): 66,928,289 (97.2%) Total basepairs processed: 3,459,545,810 bp Quality-trimmed: 3,923,654 bp (0.1%) Total written (filtered): 2,710,647,742 bp (78.4%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 384802 times No. of allowed errors: 0-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 27.4% C: 2.0% G: 33.7% T: 36.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 304422 1075906.1 0 304422 4 62656 268976.5 0 62656 5 9677 67244.1 0 9677 6 888 16811.0 0 888 7 2498 4202.8 0 2498 8 1116 1050.7 0 1116 9 539 262.7 0 50 489 10 1303 65.7 1 15 1288 11 390 16.4 1 46 344 12 184 4.1 1 1 183 13 69 1.0 1 0 69 14 71 1.0 1 0 71 15 90 1.0 1 0 90 16 204 1.0 1 0 204 17 383 1.0 1 0 383 18 259 1.0 1 0 259 19 29 1.0 1 0 29 20 9 1.0 1 0 9 21 3 1.0 1 0 3 22 3 1.0 1 0 3 23 3 1.0 1 0 3 24 2 1.0 1 0 2 25 1 1.0 1 0 1 26 1 1.0 1 0 1 27 1 1.0 1 0 1 28 1 1.0 1 0 1