This is cutadapt 2.10 with Python 3.6.7 Command line parameters: -j 1 -a AGATCGGAAGAGC -u 10 -q 20 -m 36 -O 3 -o fastq/zr1394_13_s456.fastq.gz zr1394_13_s456.fastq.gz Processing reads on 1 core in single-end mode ... Finished in 1578.71 s (24 us/read; 2.47 M reads/minute). === Summary === Total reads processed: 65,081,682 Reads with adapters: 354,479 (0.5%) Reads written (passing filters): 63,432,696 (97.5%) Total basepairs processed: 3,272,837,182 bp Quality-trimmed: 3,348,104 bp (0.1%) Total written (filtered): 2,570,106,074 bp (78.5%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 354479 times No. of allowed errors: 0-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 27.5% C: 1.7% G: 34.3% T: 36.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 280271 1016901.3 0 280271 4 58249 254225.3 0 58249 5 8364 63556.3 0 8364 6 798 15889.1 0 798 7 2561 3972.3 0 2561 8 1035 993.1 0 1035 9 508 248.3 0 36 472 10 1126 62.1 1 2 1124 11 348 15.5 1 36 312 12 174 3.9 1 1 173 13 61 1.0 1 0 61 14 55 1.0 1 0 55 15 72 1.0 1 0 72 16 185 1.0 1 0 185 17 362 1.0 1 0 362 18 275 1.0 1 0 275 19 22 1.0 1 0 22 20 5 1.0 1 0 5 21 1 1.0 1 0 1 22 1 1.0 1 0 1 23 1 1.0 1 0 1 24 1 1.0 1 0 1 26 3 1.0 1 0 3 27 1 1.0 1 0 1